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Preparation method of glycitein purified product

A technology of soybean yellowin, pure product, applied in the field of preparation of pure soybean yellowin, which can solve the problems of difficult extraction of soybean yellowin products, and achieve the effect of simple process and cost reduction

Active Publication Date: 2019-09-06
SHANDONG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is difficult to prepare pure soy isoflavones by existing methods, and because the physicochemical properties of the six substances in soy isoflavones are similar, it is even more difficult to directly extract pure soy bezein products from it

Method used

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  • Preparation method of glycitein purified product
  • Preparation method of glycitein purified product
  • Preparation method of glycitein purified product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Take 2L of soymilk water discharged during the production of soybean protein by the process of alkali extraction and acid precipitation, and let it settle naturally for 4 hours to obtain 500mL of yellow liquid in the lower layer. Add 1g NaOH to the liquid, stir it magnetically for 0.5h, let it stand for two layers, and the upper layer is 450mL , lower layer 50mL (precipitation), separate the two layers, add ethyl acetate 250mL and 50mL to the upper and lower layers in turn, respectively, magnetically stir for 0.5h to extract, separate into two layers after static, and take the upper ethyl acetate layer respectively to obtain 300mL and 50mL. The two were rotary evaporated to dryness respectively to obtain a dry residue. Add 50 mL and 10 mL of ethyl acetate to the dry residue, respectively. Vortex to dissolve as completely as possible. Centrifuge at 6000r / min for 10min, take the supernatant and spin evaporate to dryness respectively. It is detected that the upper layer ...

Embodiment 2

[0043] Take 2L of soymilk water discharged during the production of soybean protein by alkali extraction and acid precipitation process, and let it sit naturally for 5 hours to obtain 500mL of yellow liquid in the lower layer. Add 1.5g NaOH to the liquid, stir it magnetically for 45min, and divide it into two layers at rest. The upper layer is 470mL. Lower layer 30mL (with precipitation). Add 270mL and 30mL of ethyl acetate to the upper and lower layers in turn, respectively, magnetically stir each for 0.5h, stand still and separate into two layers, take the upper ethyl acetate layer respectively, and obtain 310mL and 30mL of liquid, respectively, and rotary evaporate the two to obtain dry residue , add ethyl acetate 50mL and 10mL respectively to the dry residue, vortex to dissolve as completely as possible. After centrifugation at 6000r / min for 12min, the supernatants were taken and evaporated to dryness respectively. It was detected that each of them was a single component g...

Embodiment 3

[0045] Take 2L of soymilk water discharged during the production of soybean protein by alkali extraction and acid precipitation process, and let it sit naturally for 6 hours to obtain 500mL of yellow liquid in the lower layer. 20mL (with precipitation). Add 280mL and 20mL of ethyl acetate to the upper and lower layers in turn, respectively, magnetically stir for 1h, stand still and divide into two layers, then take the upper ethyl acetate layer, respectively, to obtain 320mL and 20mL of liquid, and rotary evaporate the two to obtain dry residue, Add 50 mL and 10 mL of ethyl acetate to the dry residue, and vortex to dissolve it as completely as possible. After centrifugation at 6000r / min for 15min, the supernatants were taken and evaporated to dryness respectively. It was tested that each of them was a single component glycitein, 15.44mg and 0.28mg respectively, and the purity of glycitein was 98.2%.

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Abstract

The invention discloses a preparation method of a glycitein purified product. The method comprises the steps of soybean milk is subjected to standing settling, alkali adding treatment and ethyl acetate selectivity extraction; during alkali adding treatment, the added alkali is NaOH; the soybean milk is whey discharged in the soybean protein producing process through the alkaline extraction and acid settling technology. Degreased bean pulp is subjected to alkaline extraction so that soybean fiber and other protein can be separated, the protein and soybean isoflavones can be separated, the protein can be subjected to acid settling to be separated from isoflavones in the solution, alkali extraction and acid settling discharge solutions are mixed to obtain soybean milk with the pH close to 5-6.5, and the soybean milk contains a large amount of glycitein, and is the high-quality material for preparing glycitein.

Description

technical field [0001] The invention specifically relates to a method for preparing a pure soybean yellowin. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Glyzein, its alias: 4',7-dihydroxy-6-methoxy isoflavone, chemical formula is C 16 h 12 o 5 , together with daidzin, daidzin, genistein, genistin and daidzein are called soy isoflavones. [0004] The pharmacological effects of glycitein are: 1) It has estrogen and anti-estrogen properties; 2) It has antioxidant effect; 3) It can inhibit the activity of protein tyrosine kinase (PTK); 4) It can inhibit the topoisomerase Ⅱ activity; 5) It has the effects of inducing programmed cell death, improving the efficacy of ant...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/36C07D311/40
CPCC07D311/36C07D311/40
Inventor 刘代成尹成文
Owner SHANDONG NORMAL UNIV
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