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Method for quickly measuring six types of soy isoflavones in feed additive

A technology of soybean isoflavones and feed additives, applied in the field of analytical chemistry, can solve the problems of few related reports and slow progress in the detection and research of soybean isoflavone content, and achieve the effects of high accuracy, rapid determination and good separation effect

Inactive Publication Date: 2017-08-29
上海优久生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Research on the detection of soybean isoflavone content in feed is slow, and there are few related reports

Method used

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  • Method for quickly measuring six types of soy isoflavones in feed additive
  • Method for quickly measuring six types of soy isoflavones in feed additive
  • Method for quickly measuring six types of soy isoflavones in feed additive

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) The standard products purchased from Shanghai Tongtian Biotechnology Co., Ltd. were selected: six standard products of glycitein, daidzein, daidzein, daidzin, genistein, and genistin.

[0023] (2) Standard product preparation: Weigh daidzin, glycitin, genistin, daidzein, glycitein and genistein respectively 5mg, 4mg, 5mg, 5mg, 4mg, and 5mg of each of the six standard substances are accurate to 0.01mg, and they are dissolved in 80% ethanol to make up to 50mL, 100mL, 50mL, 50mL, 100mL and 50mL respectively. Concentrations of 100mg / L, 40mg / L, 100mg / L, 100mg / L, 40mg / L and 100mg / L were respectively prepared as standard mother solutions.

[0024] Take daidzein, daidzein, genistin, daidzein, daidzein and genistein 1mL, 2mL, 1mL, 1mL, 2mL, 1mL respectively to prepare mixed standards, the concentrations are 12.5mg / L, 10mg / L, 12.5mg / L, 12.5mg / L, 10mg / L and 12.5mg / L, as standard solution, for machine use.

[0025] (3) Sample pretreatment: Accurately weigh 0.1g of soybean iso...

Embodiment 2

[0032] Embodiment 2: Determination of the precision of 6 kinds of soybean isoflavones

[0033] Precision test: Accurately measure the prepared standard solution sample, inject samples continuously for 6 times according to the chromatographic conditions in Example 1 (4), and record the peak area of ​​the chromatogram. The precision of six kinds of soybean isoflavones is shown in Table 2.

[0034] type

[0035] Table 2 Precision of 6 soybean isoflavones

Embodiment 3

[0036] Embodiment 3: linear regression equation, correlation coefficient, linear range of 6 kinds of soybean isoflavones

[0037] Inspection of the linear range: Precisely measure the prepared standard solution sample, dilute the standard concentration to different degrees, and record the chromatogram. Taking the peak area (Y) in the serial concentration standard solution as the ordinate, and the concentration X (μg / mL) as the abscissa, linear regression was carried out, and the results are shown in Table 3.

[0038]

[0039] Table 3 Linear regression equation, correlation coefficient and linear range of six soybean isoflavones

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Abstract

The invention discloses a method for quickly measuring six types of soy isoflavones in a feed additive. The six types of soy isoflavones are respectively daidzin, glycitin, genistin, daidzein, glycitein and genistein. The method comprises the following steps of firstly, using 80% ethanol to extract the soy isoflavones in the feed additive product, centrifuging, taking a supernatant, filtering, and performing HPLC (high performance liquid chromatography) detection. The method has the advantages that the contents of six types of soy isoflavones in the feed additive product can be quickly measured; the pretreatment of a sample is simple, the analyzing speed is high, the repeatability and the accuracy are high, and an efficient and accurate analyzing method is provided for evaluating and controlling the quality of the feed additive product.

Description

technical field [0001] The invention relates to the technical field of analytical chemistry, and more specifically relates to a method for rapidly determining six soybean isoflavones in feed additives. Background technique [0002] At present, soybean isoflavones, as a new type of high-efficiency green feed additive, have been widely used in the livestock and poultry industry. They have the advantages of small dosage, quick effect, and non-toxicity. As a phytoestrogen, it is similar in structure to mammalian estrogen and has estrogen-like effects. Adding an appropriate amount of soybean isoflavones to livestock and poultry feed can enhance animal immunity, improve animal reproduction and lactation capabilities, improve poultry egg production performance, and promote growth and other physiological effects, reducing feed costs. However, the amount of isoflavone compounds added to livestock and poultry feed, the period and method of use will affect the physiological functions ...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 罗莹莹于怀龙
Owner 上海优久生物科技有限公司
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