Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Selective enrichment and separation method for dyestuff lignin in kudzu root crude extract

A technology of genistein and separation method, which is applied in the field of selective enrichment and separation of genistein in the crude extract of kudzu root, can solve the problems of solid phase extraction that have not been reported, and achieve the effect of broad application prospects

Inactive Publication Date: 2010-05-12
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, some scholars have used molecular imprinting technology for the enrichment and separation of active ingredients of traditional Chinese medicine and obtained satisfactory results (J. Chromatogr.A, 1098(2005) 66) There is no report about the molecularly imprinted solid phase extraction of genistein

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Selective enrichment and separation method for dyestuff lignin in kudzu root crude extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Pack 200 mg of genistein molecularly imprinted polymer particles with a particle size of 38-65 μm in a solid phase extraction column with an inner diameter of 1 cm and a length of 10 cm, and plug the bottom and upper parts of the column with glass frits. The extraction experiment was carried out on a self-made solid phase extraction device. Before loading, the extraction column was activated with 5ml of methanol and 5ml of acetonitrile in turn; then 1ml of 0.378mg / ml kudzu root total extract in acetonitrile solution was loaded, and then rinsed with 2ml of 50% water in acetonitrile solution and 1ml of 5% water in acetonitrile solution , and finally eluted with 2m of 10.2% TEA in methanol solution, and the eluent was blown dry with nitrogen gas, and then 1ml of 60% water in acetonitrile solution was used to make the mobile phase constant, and then the genistein was subjected to chromatographic analysis.

[0020] Comparison of chromatograms before and after molecularly imp...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a concentration and separation method of microcomponent in a complex system, in particular to the selective concentration and separation method of genistein in radix puerariaecrude extract. The steps adopted are as follows: a) genistein molecularly imprinted polymer microspheres are employed as filler to prepare solid phase extraction column; b) the solid phase extractioncolumn prepared in the step a is activated and sampled and then the solid phase extraction column is leached by using acetonitrile solution with water accounting for 49-51 percent of volume ratio andacetonitrile solution with water accounting for 4.9-5.1 percent of volume ratio in sequence, and then the leached solid phase extraction column is eluted by methanol solution with water accounting for 0.18-0.22 percent of volume ratio, thus obtaining finished products through drying the eluent. The invention has the advantages that: after the radix puerariae ethanol crude extract is extracted through molecularly imprinted solid phase, a clean chromatogram is obtained after removing most disturbance factor and the recovery ratio of the genistein reaches above 70 percent. Meanwhile, glycitein is reserved obviously. The invention has wide application prospect on the pretreatment of radix puerariae crude extract samples.

Description

technical field [0001] The invention is an enrichment and separation method for trace components in a complex system, specifically a molecular imprinted solid phase extraction technology with high selectivity and high affinity for genistein. Background technique [0002] Isoflavones are a class of natural compounds present in legumes. Many studies have shown that isoflavones have anti-cancer, anti-oxidation, scavenging free radicals, lowering cholesterol and estrogen activity (J.Agric.Food Chem., 41( 1993) 1961; Chem.Pharm.Bull., 36(1988) 2090; Lipids, 14(1979) 535; J.Nutr., 84(1964) 201; Proc.Natl.Acad.Sci.U.S.A., 90(1993 )2690). As phytoestrogens, isoflavones can not only alleviate menopausal symptoms caused by estrogen deficiency, but also have no toxic side effects (Food Res.Int., 26(1993) 131; Arch.Intem.Med., 16(2001) 1161 ), so it has been paid more and more attention. [0003] Genistein is an isoflavone compound with high estrogenic activity, which is found in low...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/36B01J20/285A61K36/48A61K125/00
Inventor 梁鑫淼王金成徐青薛兴亚章飞芳张岩
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com