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Rice endophyte (Pantoea sp. Sd-1) for efficiently degrading lignin

A technology of endophytic bacteria and alkali lignin, which can be applied in the fields of bacteria, microorganisms, biological water/sewage treatment, etc., and can solve problems such as basic research gaps.

Inactive Publication Date: 2014-02-05
HUNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among microbial resources, plant endophytes, as a type of microorganisms that are mutually beneficial and symbiotic with plants, are widely distributed and have a unique growth environment. As a new resource for plant lignin biodegradation, they are developed with strong purpose and selectivity. The research on plant endophytic bacteria that degrades lignin is basically still blank

Method used

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  • Rice endophyte (Pantoea sp. Sd-1) for efficiently degrading lignin
  • Rice endophyte (Pantoea sp. Sd-1) for efficiently degrading lignin
  • Rice endophyte (Pantoea sp. Sd-1) for efficiently degrading lignin

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1: the biological identification of bacterial strain

[0016] 1. Strain Morphological Characteristics

[0017] (1) Colony morphology characteristics: Streak inoculation of monoclonal colonies into PDA solid medium, place the plate upside down in a constant temperature incubator, incubate at 30°C for 1 to 3 days, observe the colony morphology, the colonies are light yellow, smooth, rounded edges, see attached figure 1 .

[0018] (2) Morphological characteristics of the bacteria: Gram-staining the strain and observing it with an optical microscope showed that the strain was a short rod-shaped, Gram-negative bacterium;

[0019] 2. Strain 16S rDNA Analysis

[0020] Bacterial Genomic DNA Extraction Kit was used to extract the genomic DNA of the bacterial strain, and the bacterial 16S rDNA general primer (27F, 1492R) was used to amplify the 16S rDNA fragment of the bacterial strain using the bacterial strain genomic DNA as a template, and the obtained ba...

Embodiment 2

[0025] Embodiment 2: the growth characteristic of bacterial strain in lignin liquid medium

[0026] The strains grown to the logarithmic phase were divided into 1.4 × 10 9 The inoculum amount of CFU was inoculated into liquid medium containing 3g / L alkali lignin, supplemented with 1% glucose and 0.5% peptone as the exogenous initial carbon source and nitrogen source, and cultured with shaking at 30°C and 170r / min , take a sample once a day and measure the growth OD value of the bacterial strain with a UV spectrometer, the results are shown in the attached figure 2 .

Embodiment 3

[0027] Embodiment 3: Bacterial strain is to lignin fading and degradation characteristics

[0028] The strains grown to the logarithmic phase were divided into 1.4 × 10 9 The inoculum amount of CFU was inoculated into the liquid medium containing 3g / L alkali lignin, supplemented with 1% glucose and 0.5% peptone as the exogenous initial carbon source and nitrogen source, and shaken at 30°C and 170r / min Cultured for 4 days, sampled once a day, the culture solution was centrifuged at 8000r / min for 15min, and the precipitate was discarded.

[0029]Adjust the pH of the supernatant to 7.6 with 1M NaOH, and measure the absorbance at 465nm with a UV spectrophotometer to calculate the fading rate. For the results, see image 3 .

[0030] Adjust the pH of the supernatant to 1-2 with 12M HCl, centrifuge at 12000r / min for 15min, take the precipitate, dry it at 50-60°C for 48h, weigh it to a constant weight, and calculate its degradation rate. For the results, see image 3 .

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Abstract

The invention discloses a rice endophyte (Pantoea sp. Sd-1) for efficiently degrading lignin and the preservation number of the Pantoea sp. Sd-1 is CGMCC No. 6698. The Pantoea sp. Sd-1 is a bacterial strain which can grow on a culture medium with alkali lignin as a unique carbon source. After the Pantoea sp. Sd-1 is cultured for four days in a liquid culture medium containing 3g / L alkali lignin and supplemented with 1% of glucose and 0.5% of peptone, the fading rate and the degradation of lignin respectively reach 52.38% and 69.06%. And in a liquid culture medium containing 10g / L rice straws and supplemented with 1% of glucose and 0.5% of peptone, the degradation of straws can reach 54.51 at most.

Description

technical field [0001] The invention relates to a rice endophytic bacterium isolated from rice and capable of degrading lignin with high efficiency. Background technique [0002] Resource utilization and environmental issues are two important themes of global concern. Lignocellulosic materials, such as crop straw, a by-product of the production of food crops and commercial crops, are a valuable resource for development and comprehensive utilization. Due to its complex structure, large molecular weight, and no easily hydrolyzed units, lignin is recognized as one of the most difficult chemically and biologically degradable natural polymers. In lignocellulosic materials, lignin and cellulose are closely bonded in the form of covalent bonds, and cellulose molecules are embedded in it, forming a strong natural barrier to hinder the decomposition of cellulose, and the degradation of cellulose materials into monosaccharides is the key to further fermentation. The basis for the pr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01
Inventor 朱咏华廖红东熊现秋刘选明杨晓璐马江山施小伟林建中
Owner HUNAN UNIV
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