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Pantoea sp, M3, and method for degrading malachite green

A malachite green and pantotheca technology, applied in chemical instruments and methods, applications, bacteria, etc., can solve the problems of low accuracy and lack of simultaneous detection of LMG, and achieve convenient use, stable degradation performance, and easy handling. effect of effect

Inactive Publication Date: 2009-06-03
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing microbial treatment methods mainly focus on the decolorization of MG, and the detection method also uses ultraviolet spectrophotometry, the accuracy is not high, and there is no simultaneous detection of its main metabolite LMG
There is no report on the detection of metabolite cryptomalachite green after simultaneous degradation

Method used

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  • Pantoea sp, M3, and method for degrading malachite green
  • Pantoea sp, M3, and method for degrading malachite green
  • Pantoea sp, M3, and method for degrading malachite green

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Take an appropriate amount of fish pond bottom sludge and inoculate it into the enrichment medium containing 0.04mg / L MG, and cultivate in the dark at 30°C and 150rpm. After the culture medium is turbid and the color turns lighter, transfer the suspension to the next batch of fresh culture medium, and gradually increase the concentration of MG, and repeat this until the concentration of MG reaches 5 mg / L. The mixed culture obtained after acclimatization was isolated and purified by plate coating of enriched medium, and the MG-degrading strain M3 was finally screened.

Embodiment 2

[0034] The strain was inoculated in 5mg / L malachite green enrichment medium, activated to the logarithmic phase, and the medium composition was calculated as mg / L: glucose 1000; NaCl 10; MgSO 4 10; NH 4 NO 3 100;K 2 HPO 4 100; KH 2 PO 4 100; yeast paste 60; pH7.0. Put the obtained bacterial solution into the malachite green medium containing 0.5-5 mg / L malachite green at a concentration of 2%-4% by weight, and measure the degradation rate after culturing for 5 days, which can reach 97.54%, 97.1%, and 100% respectively and 77.8%, it can be seen that the degradation effect of the bacteria on malachite green, especially the lower concentration of malachite green is better.

Embodiment 3

[0036] Different glucose concentrations were set in the degradation system to compare the degradation effect of MG ( figure 1 with figure 2 ). Depend on figure 1 with figure 2It can be seen that when the glucose concentration is 0 mg / L, M3 grows slowly, and the MG concentration does not decrease significantly after 10 days of culture, indicating that M3 cannot grow and metabolize with MG as the only carbon source, and its slow growth and degradation may be due to the initial exposure to Occurs due to the influence of the incoming bacterial suspension. At higher glucose concentrations of 500mg / L and 1000mg / L, the degradation rate of MG was basically the same as the growth of the strain. The strain reached the stable growth phase within 1d, and could degrade MG almost completely within 2d. Therefore, glucose is used as the carbon source of the growth substrate of the strain, and its concentration has a certain influence on the growth of microorganisms. The higher the conce...

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Abstract

This invention discloses Pantoea sp. M3 (CCTCC No.M207022) with the ability to degrade malachite green. Logarithmic phase Pantoea sp. M3 2-4 wt. % is added into sterile degradation medium containing 0.5-5 mg / L malachite green, and cultured at 20-30 deg.C for 5-10 days to degrade malachite green. The degradation medium with a pH value of 6.0-8.0 comprises: glucose 0.5-1 g / L, NaCl 0.01-0.1 g / L, MgSO4 0.01-0.1 g / L, NH4NO3 0.02-0.1 g / L, K2HPO4 0.01-0.1 g / L, KH2PO4 0.01-0.1 g / L, and yeast extractum 0.06-0.3 g / L. Pantoea sp. M3 can effectively remove residual malachite green and leucomalachite green in water body for fishery. The degradation rate of Pantoea sp. M3 for 0.5-2 mg / L malachite green is higher than 90%.

Description

technical field [0001] The present invention relates to a new bacterial strain of the genus Pantoea, and also relates to the application of the bacterial strain in degrading malachite green. Background technique [0002] Malachite green (MG) has been widely used in aquaculture as a fungicide. After MG enters the organism, it transforms into leucomalachite green (LMG). MG and LMG accumulate in organism tissues, are not easy to metabolize, and have carcinogenic and mutagenic effects. Canada, the European Union and other countries have stipulated that the detection of MG and its LMG in edible aquatic products is prohibited. In May 2002, my country included MG in the "List of Veterinary Drugs and Their Compounds Prohibited for Food Animals". Due to the slow metabolic cycle of MG, it is difficult to eliminate it in general fish ponds for many years. Therefore, great attention should also be paid to the residue of MG in fishery waters. , [0003] At present, the main methods...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C02F1/58A01K63/04
Inventor 杨柳燕任倩张彤晴潘建林蒋丽娟吴光红宋炜肖琳林海周刚
Owner NANJING UNIV
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