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Organic solid waste high-temperature aerobic composting strain and application thereof

An organic solid waste and aerobic composting technology, applied in the field of environmental microorganisms, can solve the problems of incomplete fermentation, short high temperature period, nutrient loss, etc., and achieve the effects of complete decomposition, decomposing, harmless, and high nutrient content.

Active Publication Date: 2021-05-07
SHANGHAI ADVANCED RES INST CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional aerobic composting technology has problems such as short high temperature period, long composting cycle, incomplete fermentation, serious nutrient loss, and low material maturity

Method used

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  • Organic solid waste high-temperature aerobic composting strain and application thereof
  • Organic solid waste high-temperature aerobic composting strain and application thereof
  • Organic solid waste high-temperature aerobic composting strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0036] The third aspect of the present invention provides the preparation method of the liquid bacterial agent, the preparation method includes the following steps: Pantoea Pantoea sp . The pure strain of BK-PP311 is inoculated in the liquid medium for cultivation, and the liquid bacterial agent is obtained after the cultivation is completed.

[0037] In one embodiment, Pantoea Pantoea sp . After the pure strain of BK-PP311 is inoculated into a liquid medium for cultivation, the obtained culture medium is then inoculated into another liquid medium for expanded cultivation, and a liquid bacterial agent is obtained after the cultivation is completed.

[0038] In one embodiment, the temperature of cultivation is 40-60°C.

[0039] In one embodiment, the stirring speed of the cultivation is 150-230 r / min.

[0040] In one embodiment, Pantoea Pantoea sp . The pure strain of BK-PP311 was inoculated into liquid medium and cultured for 12-16 h, and then inoculated into another ...

Embodiment 1

[0065] Example 1: Isolation, screening and performance determination of bacterial strain BK-PP311

[0066]The separation and purification process of the bacterial strain BK-PP311 of the present invention: the test sample was collected from a high-temperature composting equipment of a cattle farm, mixed with sterile water, and then diluted in a gradient, and 200 μL of the diluted solution of different gradients was applied to the separation After culturing in a constant temperature incubator at 60 °C for 24-48 hours on the purified medium plate, single colonies with different shapes and sizes were picked, streaked and purified, and numbered and preserved. A total of 26 strains were obtained through preliminary screening.

[0067] The first re-screening: the 26 strains of pure bacteria obtained from the primary screening were inoculated onto the protease screening medium and the amylase screening medium by a spot-splicing method, and the re-screening of the enzyme-producing abili...

Embodiment 2

[0073] Embodiment 2: the bacterial agent preparation of bacterial strain BK-PP311

[0074] Preparation of liquid bacterial agent: BK-PP311 pure strain was inoculated in 10 mL seed medium, cultured in a constant temperature shaker at 50 °C and 180 r / min for 16 h, and then inoculated with 1% inoculum The liquid inoculum of the strain can be obtained by expanding the culture in the same liquid medium and culturing at 50°C and 180 r / min for 16 h.

[0075] Preparation of solid bacterial agent: it is prepared by mixing starch and liquid bacterial agent of the strain according to the mass ratio of 1:5, and spray drying.

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Abstract

The invention belongs to the technical field of environmental microorganisms, and particularly relates to an organic solid waste aerobic composting strain. The organic solid waste aerobic composting strain is identified as Pantoea sp., and has the preservation number of CCTCC NO: M 2020973. The pantoea Pantoeasp.BK-PP311 is resistant to high temperature, has the capacity of efficiently degrading organic matters and quickly decomposing, and can be applied to the high-temperature aerobic composting treatment process of organic solid wastes such as food wastes, kitchen wastes, fruit and vegetable wastes, livestock and poultry breeding feces, municipal sludge and the like, so that quick composting and resource utilization of the organic solid wastes are realized.

Description

technical field [0001] The invention relates to the technical field of environmental microorganisms, in particular to a high-temperature aerobic composting strain of organic solid waste and its application. Background technique [0002] In recent years, with the growth of population and the development of industry and agriculture, the output of organic solid waste has increased year by year. Sources of organic solid waste mainly include catering waste, kitchen waste, fruit and vegetable waste, livestock and poultry manure, municipal sludge, etc. At present, a large amount of organic solid waste has not been fully utilized, resulting in a serious waste of resources. Therefore, how to improve the resource utilization rate of organic solid waste and realize the reduction, harmless treatment and resource utilization of organic solid waste is imminent. [0003] At present, the resource utilization of organic solid waste includes energy, fertilizer, and feed. Among them, the co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F17/20C05F15/00C05F3/00C05F9/04C05F5/00C05F7/00C12R1/01
CPCC05F3/00C05F5/00C05F5/002C05F7/00C05F9/04C12N1/20C05F17/20C05F9/00Y02W30/40Y02A40/20
Inventor 刘莉吉雨霁史吉平王娜张斯颖陈晓淼肖梦瑶
Owner SHANGHAI ADVANCED RES INST CHINESE ACADEMY OF SCI
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