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Pantoea for conversion of resveratrol to ε-glutin and uses thereof

A technology of resveratrol and viniferin, which is applied in the directions of microorganism-based methods, bacteria, biochemical equipment and methods, etc., can solve the problems of low content of epsilon-staphyllin, difficulty in extraction and separation, and many by-products, etc. Low cost, high yield, high yield and stable effect

Active Publication Date: 2022-01-04
天津市尖峰天然产物研究开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In recent years, there have been many researches on the functional activity of ε-glutinin abroad, and related grape vine extract products have been listed. The content of stilben compound is 30-50%, of which the content of ε-glucose is only 10-20%
At the same time, there are few domestic studies on ε-glutin, and the research methods focus on grapevine extraction, purification, and refining to obtain the main product as grapevine and / or trimer stilbene; but because grapevine and trimer stilbene Compounds have various structural formulas, so their products should be complexes of various glucosinolates and complexes of various trimer stilbenes
However, the current biosynthesis and chemical synthesis methods of ε-glutin have defects to varying degrees: low yield, many by-products, certain pollution, etc.
[0006] However, there is no relevant report in the prior art that directly uses endophytes to convert resveratrol into ε-viniferin

Method used

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  • Pantoea for conversion of resveratrol to ε-glutin and uses thereof
  • Pantoea for conversion of resveratrol to ε-glutin and uses thereof
  • Pantoea for conversion of resveratrol to ε-glutin and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] Embodiment 1 uses Pantoea B2 bacterial strain of the present invention to prepare ε-glucosin

[0071] Using the Pantoea B2 strain isolated from the laboratory as the starting strain, the following method was used to prepare ε-glutin:

[0072] 1) Preparation of beef extract peptone medium:

[0073] Weigh 0.3g, 1g, and 0.5g of beef extract, peptone, and NaCl respectively, and fully dissolve them in 100ml of deionized water to obtain beef extract-peptone liquid medium. The dissolved culture medium was packed in a 250ml Erlenmeyer flask, and the packed culture medium was sealed at 1.06kg / cm 2 Sterilize under pressure at 121°C for 30 minutes. After sterilization, let it cool down and use it to cultivate and make fermentation broth and convert resveratrol. Add 3g of agar to every 100ml of beef extract-peptone liquid medium, and heat to fully dissolve to obtain beef-extract-peptone solid medium. The dissolved culture medium was packed in a 250ml Erlenmeyer flask, and the...

Embodiment 2

[0097] Embodiment 2 uses Pantoea B2 bacterial strain of the present invention to prepare ε-glucosin

[0098] Using the strain isolated from the laboratory as the starting strain, the following method was used to prepare ε-glucosin:

[0099] 1) Preparation of beef extract peptone medium:

[0100] Weigh 0.3g, 1g, and 0.5g of beef extract, peptone, and NaCl respectively, and fully dissolve them in 100ml of deionized water to obtain beef extract-peptone liquid medium. The dissolved culture medium was packed in a 250ml Erlenmeyer flask, and the packed culture medium was sealed at 1.06kg / cm 2Sterilize under pressure at 121°C for 30 minutes. After sterilization, let it cool down and use it to cultivate and make fermentation broth and convert resveratrol. Add 3g of agar to every 100ml of beef extract-peptone liquid medium, and heat to fully dissolve to obtain beef-extract-peptone solid medium. The dissolved culture medium was packed in a 250ml Erlenmeyer flask, and the packed cu...

Embodiment 3

[0111] Example 3 Preparation of ε-glucosin using Pantoea B2 strain of the present invention

[0112] Using the strain isolated from the laboratory as the starting strain, the following method was used to prepare ε-glucosin:

[0113] 1) Preparation of beef extract peptone medium:

[0114] Weigh 0.3g, 1g, and 0.5g of beef extract, peptone, and NaCl respectively, and fully dissolve them in 100ml of deionized water to obtain beef extract-peptone liquid medium. The dissolved culture medium was packed in a 250ml Erlenmeyer flask, and the packed culture medium was sealed at 1.06kg / cm 2 Sterilize under pressure at 121°C for 30 minutes. After sterilization, let it cool down and use it to cultivate and make fermentation broth and convert resveratrol. Add 3g of agar to every 100ml of beef extract-peptone liquid medium, and heat to fully dissolve to obtain beef-extract-peptone solid medium. The dissolved culture medium was packed in a 250ml Erlenmeyer flask, and the packed culture m...

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Abstract

The invention provides a pantoea for converting resveratrol into epsilon-staphylin and its application, and the preservation number of the pantoea is CGMCC No.13605. The present invention also provides a method for preparing epsilon-staphylin based on the pantoea. Compared with the traditional transformation method, the pantoea provided by the present invention has high yield, no by-products, and no pollution to the environment; the strain Pantoea sp B2 of the present invention, CGMCC No.13605, has high yield and stable character The characteristics of this strain have remained stable after 20 generations of passages.

Description

technical field [0001] The invention belongs to the technical field of microorganism transformation, and relates to a pantoea for converting resveratrol into resveratrol dimer ε-viniferin (ε-viniferin) and its application. Background technique [0002] Drinking wine is known to be good for your health, and research suggests it is because of the antioxidant resveratrol in wine. However, recent studies have shown that some wines do not contain resveratrol, but contain resveratrol dimers. Resveratrol dimer is a substance contained in the antitoxin secreted by grapes when they are infected by Botrytis cinerea during their growth. Resveratrol dimer, also known as grapevine, has a variety of structural formula types, such as ε-viniferin (structural formula shown in formula I) and δ-viniferin, etc., mainly exists in grapes, resveratrol dimer It is formed by the oxidation of resveratrol by 4-hydroxystilbene peroxidase. Among them, studies have reported that ε-viniferin has strong...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P17/04C12R1/01
CPCC12P17/04C12R2001/01C12N1/205
Inventor 刘岱琳张晨曦贾秀娟赵艳敏王文仲
Owner 天津市尖峰天然产物研究开发有限公司
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