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Strain capable of degrading residual quinclorac in soil

A technology of quinclorac and bacterial strains, which is applied in the field of microbial treatment of environmental pollution, can solve the problems of tobacco production and output value reduction, and achieve the effects of reducing economic losses and protecting the environment

Inactive Publication Date: 2017-09-29
FUJIAN AGRI & FORESTRY UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problem that the above-mentioned quinclorac remains in the soil and lead to the reduction of tobacco yield and output value, the present invention provides a bacterial strain capable of degrading the residual quinclorac in the soil

Method used

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  • Strain capable of degrading residual quinclorac in soil
  • Strain capable of degrading residual quinclorac in soil
  • Strain capable of degrading residual quinclorac in soil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Screening of bacterial strain J7

[0027] In this experiment, the soil was collected from the tobacco field, and the enrichment method was used to screen the microbial strains that could degrade quinclorac. The process was as follows: figure 1 shown. In this test, a strain that can degrade quinclorac in soil was screened and named J7; the screening process is as follows:

[0028] (1) Enrichment of quinclorac-degrading bacteria: Mix 0.1 g of soil with 100 ml MM medium, add quinclorac to make the final concentration 100 µg / ml, and culture at 30°C, 150 r / min 1 week; take 1 ml of the culture solution and add it to 100 ml of fresh medium containing the same concentration of herbicide, and at the same time streak the solid medium to confirm the growth of bacteria, and cultivate under the same conditions for 1 week; after 10 consecutive subcultures, the Streak the plates to obtain single colony strains. The bacterium is a gram-negative bacterium, the colony is li...

Embodiment 2

[0034] Example 2 Molecular identification

[0035]Using the bacterial 16S rDNA universal primer fD2 / rP1, the 16s rDNA of the strain was amplified by colony PCR and sequenced for comparative analysis to identify the strain:

[0036] fD2: 5'-AGAGTTTGATCATGGCTCAG-3',

[0037] rP1: 5'-ACGGTTACCTTGTTACGACTT-3';

[0038] PCR reaction system: The PCR reaction system is: 1×PCR MIX reaction solution (Quanshijin Biotechnology Co., Ltd., Beijing), 0.4 μM primers, and use a toothpick to pick a small number of colonies as templates;

[0039] PCR reaction program: 32 cycles of pre-denaturation at 95°C for 4 min, each cycle including denaturation at 95°C for 30 s, annealing at 58°C for 30 s, and extension at 72°C for 1 min; finally, extension at 72°C for 10 min.

[0040] The PCR product J7-16S rDNA was verified by agarose gel electrophoresis, and DNA was recovered and sequenced, and the sequencing results were compared with the Genbank database. The results show that the sequence of the s...

Embodiment 3

[0041] Example 3 Tobacco Seedling Experiment: Control Effect of Bacterial Strain J7

[0042] (1) Determination of the concentration of phytotoxicity caused by the medicinal solution in the soil to the tobacco seedlings: respectively prepare nutrient soil containing 0, 0.001, 0.01 and 0.1 µg / g quinclorac medicinal solution, stir evenly and put the same amount into the flowerpot , transplanted into tobacco seedlings with similar growth conditions, observed the growth of tobacco after 40 days, and measured parameters such as plant height, leaf area and leaf number of tobacco seedlings. Each treatment was repeated 5 times. The results showed that when quinclorac was 0.001µg / g soil, the phytotoxicity was not obvious; when quinclorac was 0.01µg / g and 0.1µg / g soil, the phytotoxicity was obvious ( Figure 6 ). Phytotoxicity is mainly manifested in the reduction of leaf area, and at the same time has a certain impact on plant height, but has no obvious inhibitory effect on leaf numbe...

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Abstract

The invention discloses quinclorac degradation bacterium and application thereof. The quinclorac degradation bacterium J7 provided by the invention belongs to pantoea sp. and is preserved in China Center for Type Culture Collection (CCTCC); the preservation date is March 16, 2017 and the preservation number is CCTCC M2017131; the quinclorac degradation bacterium is identified to be pantoea sp. by 16S rDNA. The bacterium is a Gram-negative bacterium and has a light yellow bacterial colony, a smooth and round surface and an ordered edge. The bacterium has a function of degrading quinclorac and can be used for preventing and controlling phytotoxicity, caused by the quinclorac, on tobaccos.

Description

technical field [0001] The invention belongs to the technical field of microbial treatment of environmental pollution, and in particular relates to a bacterial strain capable of degrading soil residual quinclorac. Background technique [0002] tobacco( Nicotiana tabacum ) is an annual or limited perennial herb of the family Solanaceae, which originated in South America and is currently planted all over the world. Tobacco is a special economic crop. Since my country implemented the tobacco monopoly system in 1982, the tobacco industry has developed rapidly and has become an important pillar industry of the national economy. Tobacco is grown in all provinces in the north and south of my country, and its main purpose is to process tobacco leaves to produce cigarettes. Therefore, high-quality tobacco leaves are an important goal of tobacco production, and how to ensure high-quality tobacco leaves is also an important issue that tobacco farmers care about. In Fujian, tobacco c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/01
CPCB09C1/10C12N1/205C12R2001/01
Inventor 陈凤平胡杨冯建军周挺顾钢徐进詹家绥
Owner FUJIAN AGRI & FORESTRY UNIV
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