The invention provides a biological alcoholization method for tobacco leaves. Strains which facilitate acceleration of alcoholization are cultured and fermented,
fermentation liquor is subjected to plate-frame
filtration and vacuum concentration and then prepared into spray-
coating liquid by adding a protection agent, and the spray-
coating liquid is uniformly sprayed to the surfaces of the tobaccoleaves. According to the biological alcoholization method for the tobacco leaves, the mixed strains are used for seed culture, afterwards,
fermentation is conducted,
lignin,
starch and proteins in tobacco stem extracts are used as inducing substrates of enzymes, generated
laccase is highly targeted to degradation of the
lignin in tobacco stems, the degradation efficiency is high, and the contentof the
lignin in the tobacco stems is reduced; the
starch, the proteins, pectins,
hemicellulose and other impurities which affect the smoking taste in the tobacco stems generate the corresponding enzymes through the inducing substrates in the tobacco stem extracts, after the spray-
coating liquid is sprayed to the tobacco leaves, the corresponding substrates are degraded, the time for the natural alcoholization process is shortened, and the taste is better and more mellow.