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Method for extracting bioflocculant

A biological flocculant and flocculant technology, applied in the direction of biochemical equipment and methods, microorganisms, microorganisms, etc., can solve the problems of high cost and large dosage, and achieve the effects of low cost, easy operation and convenient material collection

Inactive Publication Date: 2012-04-04
SHANGHAI HAIDI GARDENING
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The biggest obstacle to the application of microbial flocculants is the large amount of use and high cost. Therefore, the screening of high-efficiency microbial flocculant-producing bacteria, the improvement of flocculation activity, and the reduction of the amount and cost of flocculants are the problems that biological flocculants need to solve at present.

Method used

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  • Method for extracting bioflocculant
  • Method for extracting bioflocculant
  • Method for extracting bioflocculant

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Embodiment Construction

[0019] The technical solution will be explained in detail below in conjunction with specific embodiments of the present invention:

[0020] The preparation embodiment of flocculant:

[0021] The strains are derived from the activated sludge of the aeration tank of the sewage plant in the east area of ​​Yangpu District, Shanghai. The basal medium: beef extract peptone medium; the screening medium (also known as glucose medium): glucose 20g, KH 2 PO 4 0.2g, K 2 HPO 4 0.5g, (NH 4 ) 2 SO 4 0.2g, NaCl 0.1g, urea 0.5g, yeast extract 0.5g, MgSO4 7H 2 O 0.2 g (2% agar) was added to 1000 mL of water, and several portions of medium with a pH of 6.0 to 9.0 were prepared respectively, and sterilized at 112° C. for 30 min.

[0022] 1. Bacterial strain screening: after the activated sludge is diluted, put it on a constant temperature shaker at 30°C, enrich and cultivate it at 160r / min for 3 days, obtain a single colony by the dilution plate coating method, and number it after puri...

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Abstract

The invention relates to a method for extracting a bioflocculant. The method is characterized by extracting the flocculant from activated sludge, has the advantages of wide source and low cost, comprising the following steps: screening the activated sludge and extracting bacterial strains with best activity, culturing in a glucose medium, and then carrying out extraction on the supernatant liquor in the medium by acetone process, ethanol process or CTAB process. The obtained flocculant has high activity, high yield, no secondary pollution to the environment, and very good potential of large scale production.

Description

technical field [0001] The invention relates to an extraction method from biological flocculants. Background technique [0002] Microbial flocculants are extracellular metabolites with flocculation activity produced by microorganisms under specific culture conditions when they grow and metabolize to a certain stage. The main components are glycoproteins, polysaccharides, proteins, DNA, etc. In the late 1980s, Ryuichiro Kurane of Japan screened out the strain of Rhodococcus erythropolis S-1 (R Erythropolis S-1), and named the microbial flocculant produced by the strain NOC-1. The best microbial flocculant has good flocculation and turbidity removal effects on various wastewaters. Different from traditional chemical flocculants, microbial flocculants not only have good flocculation and sedimentation performance, but also are safe, non-toxic, biodegradable, and have no adverse effects on the ecological environment; at the same time, there are many types of microorganisms that ...

Claims

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Application Information

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IPC IPC(8): C12P1/00C12N1/00C02F1/56
Inventor 丁国才
Owner SHANGHAI HAIDI GARDENING
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