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Kit for separating and identifying campylobacter rectus as well as preparation and application for kit

A campylobacter and kit technology, which is applied to the kits, preparation and application fields for the isolation and identification of Campylobacter, can solve the problems of high missed detection rate, high cost, complicated identification steps, etc., to improve work efficiency, easy to master and Accept and reduce the effect of random errors

Inactive Publication Date: 2012-12-12
SHANGHAI MUNICIPAL CENT FOR DISEASE CONTROL & PREVENTION
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AI Technical Summary

Problems solved by technology

It mainly solves the technical problems of high missed detection rate, complicated identification steps and high cost, and unintuitive screening efficiency in the isolation methods of Campylobacter jejuni and non-Campylobacter jejuni.

Method used

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Effect test

Embodiment Construction

[0014] First, the kit is prepared according to the content of the technical plan. The user chooses to use the enrichment separation step or the selective plate separation step directly according to different samples, and selects 3-5 suspected colonies to use the combined identification test to complete the screening test.

[0015] 1. Explanation, collection requirements and preprocessing methods of different test materials.

[0016] 1) Feces: including human (patients and carriers), poultry (livestock) and other animal feces samples, collect about 1g (thumb size) as fresh as possible, and about 1ml of liquid feces, and inoculate and culture within 1h-2h. If it cannot be detected in a short time, it should be stored in a commercial transport medium and sent to the laboratory within 24 hours. The direct separation method is generally used for fecal samples, that is, the specimen is picked up with a cotton swab (swab) and directly inoculated on a KARMALI plate and then separated ...

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Abstract

The invention relates to a kit for separating and identifying campylobacter rectus as well as a preparation and an application for the kit, and mainly solves technical problems of high omission factor, complex identifying steps, higher cost and insufficiently visualized screened results in the existing method for separating the campylobacter rectus, such as campylobacter jejuni and non campylobacter jejuni. The kit for separating and identifying the campylobacter rectus has the technical scheme that: the kit comprises a selective enrichment broth I, a selective enrichment broth II, an improved Karmali agar plate, a Columbia blood plate, a microaerophilic gas generation bag, a gram staining solution, an oxidase reagent, a hippuric acid paper sheet and a hippuric acid enzyme reaction reagent. The campylobacter jejuni or non campylobacter jejuni is identified through a reaction combination test of a substrate and a typical colonial morphologic, simple biochemical and specific enzyme of the campylobacter rectus correspondingly grown in a culture medium for selective separation.

Description

technical field [0001] The invention relates to a kit, preparation and application for the isolation and identification of Campylobacter, a method for synchronous isolation and identification of Campylobacter including Campylobacter jejuni or non-Campylobacter jejuni in Campylobacter genus, using a selective isolation medium to grow Campylobacter correspondingly Typical colony morphology, simple biochemical and specific enzyme-substrate reaction combined test kits and methods for identifying Campylobacter jejuni or other Campylobacter jejuni. Background technique [0002] There are currently more than 30 species of Campylobacter, which are widely parasitic in the intestines (colon or jejunum) of birds in nature and farmed poultry and livestock animals. They colonize for a long time and pollute the environment and food with excreta. The jejunum Campylobacter, Campylobacter coli, and Campylobacter seagull are known pathogenic bacteria that are clearly quarantined for poultry a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/26C12Q1/04
CPCY02A50/30
Inventor 许学斌袁政安金汇明
Owner SHANGHAI MUNICIPAL CENT FOR DISEASE CONTROL & PREVENTION
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