The invention discloses a method for monitoring
salmonella. The method for monitoring
salmonella comprises the following steps that an appropriate amount of a sample to be detected is mixed with a BWPsolution, the mixture is ground / stirred to obtain a suspension /
mixed solution, insoluble matter is removed by
centrifugation, then the
bacteria is washed with normal
saline, lytic cells are boiled, asupernatant is taken through
centrifugation, the supernatant is placed in a sterile
test tube, enrichment culturing is performed in an
incubator for 8-10 hours at 36 DEG C, a BPW
liquid culture is inoculated into selective
enrichment broth SC of the
salmonella, an enrichment culturing solution is inoculated on a SS medium in a one-
ring mode, and medium-sized, circular colonies with black centerson the SS medium are selected for
pure culture, and the purely cultured colonies are simultaneously subjected to dual PCR and biochemical identification. The detection time of the salmonella is shortened, the detection process is accelerated, the operation complexity is reduced, the labor intensity is reduced, the detection cost is reduced, and fast, simple, and sensitive detection of a target object is achieved, the dual PCR and biochemical identification are simultaneously carried out, so that the accuracy is improved.