Separation and purification method of sulfate-reducing bacteria in oilfield production water

A separation and purification, sulfate technology, applied in the field of petroleum microorganisms, can solve the problems of small scope of application, complicated operation, limitations of microorganism separation and purification technology, etc., and achieve the effect of convenient operation, simple process and easy operation.

Inactive Publication Date: 2011-11-30
E TECH ENERGY TECH DEV CORP
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to solve the problems of limitations, small scope of application and complicated operation of the existing microbial separation and purification technology, and provide a method with simple process, convenient operation, easy-to-obtain materials, economical and practical, efficient and quick, and good separation and purification effects. Separation and purification method of sulfate-reducing bacteria in oilfield production water with characteristics such as

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  • Separation and purification method of sulfate-reducing bacteria in oilfield production water
  • Separation and purification method of sulfate-reducing bacteria in oilfield production water
  • Separation and purification method of sulfate-reducing bacteria in oilfield production water

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Embodiment 1

[0025] The separation and purification method of sulfate-reducing bacteria in oilfield production water is to collect oilfield production water at the oilfield site, seal it, and send it back to the laboratory for low-temperature storage in time. Take 1mL of oilfield production water and add it to the specific liquid medium of SRB, 30~37 ℃ constant temperature anaerobic enrichment culture until the liquid enrichment medium turns black and emits the smell of rotten eggs of hydrogen sulfide. Take 1mL of SRB-enriched culture solution for gradient dilution to an appropriate dilution gradient, and then apply the diluted bacterial enrichment solution on a solid interlayer plate, that is, pour the specific medium of solid SRB into the lid of the petri dish, cool and solidify to form For the lower medium, draw 0.2 mL of the appropriate diluted gradient enrichment solution and spread it evenly on the lower medium, then pour the upper layer of solid SRB-specific medium, and quickly cover...

Embodiment 2

[0027] Embodiment 2 The concrete process of separating and purifying sulfate-reducing bacteria in the production water of the primary production separator in the oil field production treatment process is as follows:

[0028] After about 3 minutes after the water phase outlet of the on-site primary separator discharged the residual liquid in the pipeline, about 500ml of water samples were collected with a sealed bottle, and sent back to the laboratory in time for low-temperature storage.

[0029] In the aseptic operation table (or aseptic room), take 1ml of water sample and add it to 9ml of SRB-specific liquid medium, and carry out constant temperature anaerobic enrichment culture in an anaerobic bottle at 35°C. After 3 days After the enrichment culture, the SRB liquid enrichment medium turns black and emits the smell of rotten eggs of hydrogen sulfide.

[0030] Take 1 mL of the obtained SRB enriched culture solution and carry out 10 -1 ~10 -3 For gradient dilution, pour the ...

Embodiment 3

[0034] The specific process of isolating and purifying sulfate-reducing bacteria in oilfield single well production fluid is as follows:

[0035] After draining the residual liquid in the pipeline for about 2 minutes at the sampling port of the oil well production fluid on site, collect about 1.5 L of oil and water samples with a liquid separation bucket, then separate the oil and water, and collect about 500 ml of water samples with a sealed bottle, and send them back to the experiment in time room for cryopreservation.

[0036] In the aseptic operation table (or aseptic room), take 1ml of water sample and add it to 9ml of SRB-specific liquid medium, and carry out constant temperature anaerobic enrichment culture in an anaerobic bottle at 37°C. After 3 days After the enrichment culture, the SRB liquid enrichment medium turns black and emits the smell of rotten eggs of hydrogen sulfide.

[0037] Take 1 mL of the obtained SRB enrichment culture solution and carry out 10 -1 ~1...

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Abstract

The invention relates to a method for separating and purifying sulfate-reducing bacteria in oilfield production water: adding oilfield production water into a liquid culture medium for sulfate-reducing bacteria, and carrying out anaerobic enrichment culture at a constant temperature of 30-37°C; enriching culture solution for sulfate-reducing bacteria Carry out gradient dilution and solid interlayer plate coating, pour the culture medium of solid sulfate-reducing bacteria into the lid of the culture dish, cool and solidify to form the lower layer of culture medium, absorb the diluted enriched bacteria solution and spread it evenly on the lower layer for cultivation Then pour the upper layer of solid sulfate-reducing bacteria culture medium, and cover the bottom of the petri dish on the upper layer of culture medium for constant temperature cultivation; when a single colony appears to turn the medium black, pick the single colony into sulfate-reducing bacteria The liquid enrichment medium is used for enrichment culture, and the strain whose culture medium becomes the darkest is taken and the steps of sandwich plate to liquid enrichment culture are repeated three times to obtain pure strains of sulfate-reducing bacteria. The invention has the advantages of convenient operation, readily available materials, good separation and purification effects and the like.

Description

technical field [0001] The invention belongs to the technical field of petroleum microbes, in particular to a method for separating and purifying sulfate-reducing bacteria in oil field production water. Background technique [0002] Sulfate-reducing bacteria (SRB) are microorganisms that use sulfate or other oxidized sulfides in oilfield production water as electron acceptors to dissimilate organic substances. 4 2- reduced to H 2 S, which in turn changes the pH of the oilfield water quality and the corrosion state of the metal, is an important factor for the significant increase in the metal corrosion rate, and brings many harmful effects such as corrosion and blockage to the oilfield pipeline. [0003] Separation and purification of SRB to obtain a pure SRB single strain can provide an important theoretical basis for the analysis of the bacterial community structure of the oil field environment and the evaluation of the corrosion status and water quality. [0004] The ex...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02
Inventor 胡欣
Owner E TECH ENERGY TECH DEV CORP
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