Campylobacter jejuni detection method with liquid chip

Abstract

The invention which relates to a Campylobacter jejuni detection method with a liquid chip belongs to the technical fields of immunization and food health detection. The invention which aims to provide a liquid chip utilization technology provides a Campylobacter jejuni detection method to realize the rapid Campylobacter jejuni detection. The liquid chip which is prepared in the invention is applied to detect Campylobacter jejuni. Compared with several other detection methods, the Campylobacter jejuni detection method with the liquid chip of the invention has the advantages of bidirectional high throughout of the detection sample number and the detection project (a large number of samples can simultaneously undergo detection projects with the number of high to ninety six), good flexibility, high sensitivity, good signal / noise ratio, simple operation, wide standard curve range, wide application range and the like. The liquid chip technology has a wide application prospect in fields of clinical diagnosis, infectious disease diagnosis by veterinarians, food microbe detection and the like when the technology is used as an advanced operation platform for the bioinformatics. The method which allows the rapid Campylobacter jejuni detection to be realized makes a contribution to the food safety.

Description

technical field [0001] The invention belongs to the technical field of immune technology and food sanitation detection. Background technique [0002] Campylobacter jejuni is a pathogenic bacterium that has received widespread attention at home and abroad since the 1980s. It can cause a variety of diseases in humans and animals. It is considered to be the main pathogenic bacterium that causes bacterial diarrhea in humans all over the world, and its infection rate for humans is no less than that of Salmonella and Shigella are important foodborne pathogens. The source of infection is mainly animals and their contaminated food. Campylobacter is widely distributed in various animals, among which poultry, wild fowl and livestock carry the most bacteria, followed by campylobacter isolated from rodents. The current methods for the detection of Campylobacter jejuni in food (whether classical methods, molecular biology methods, immunological methods, etc.) are characterized by cumber...

AI Technical Summary

Problems solved by technology

At present, the methods for the detection of Campylobacter jejuni in food (whether classical methods, molecular biology methods, immunological methods, etc.) have the characteristics of cumbersome operation and long time, and only one kind of bacteria can be detected at a time.

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