The invention belongs to the analysis field of trace
pollutant in environment, in particular relates to a method for measuring antibiotic in animal
excretion. In the method, EDTA-McIlvaine buffering solution and
acetonitrile acidulated by
formic acid are taken as extracting solution which is extracted by a
microwave assisted extractor, and then the steps such as
solid phase extraction
cartridge, purification and concentration are carried out, finally, liquid chromatogram-
mass spectra /
mass spectra (LC-MS / MS) is utilized for analytic determination, thereby accurately and rapidly analyzing the antibiotic in animal
excretion. The concrete analytic determination method is as follows: taking a given amount of frozen and dried
excretion, putting the excretion into a
polytetrafluoroethylene extracting tank, adding the EDTA-McIlvaine buffering solution and
acetonitrile acidulated by
formic acid into the extracting tank, utilizing the debugged
microwave assisted extractor for extraction, and then carrying out
solid phase extraction
cartridge, concentration and purification after optimization,
drying by
nitrogen, fixing volume, and at last, utilizing regulated HPLC-MS / MS for accurate measurement. The measuring method of the invention is simple and rapid, and has the characteristics of high
recovery rate, low
detection limit, high sensitivity, matrix
interference resistance and the like.