172 results about "Quaternized chitosan" patented technology

The invention relates to a method for preparing an antibacterial film of a quaternarized chitosan iodine complex. The method comprises the following steps: inserting quaternary ammonium salt on the hydroxyl group or amino group of a chitosan structure unit to prepare a quaternarized chitosan derivative; soaking a blend film of the quaternarized chitosan derivative and gelatin or poval into a simple substance iodine solution for 4-6 hours to obtain the antibacterial film of the quaternarized chitosan / gelatin or poval iodine complex, or directly preparing a quaternary ammonium salt chitosan iodine complex from the quaternarized chitosan derivative and simple substance iodine, and then mixing the quaternary ammonium salt chitosan iodine complex with gelatin or poval to prepare the blend antibacterial film containing iodine. By using the method, the water solubility and the biological activity of chitosan can be improved; simple substance iodine is stabilized and complexed by utilizing the affinity of a quaternary ammonium salt group and an iodine molecule; according to the different positions of a human body, the prepared antibacterial film can be compounded with other materials; a chitosan base has hemostasis, antiphlogosis and tissue repair functions on human body wound, can reduce the stimulation of iodine on the wound and controls the release of iodine, and the double effects of the chitosan base and iodine provide an ideal curing environment for a wound surface.

The invention discloses an antibacterial adhesion injectable hydrogel dressing, a preparation method and applications thereof, and belongs to the technical field of biodegradable biomedical materials.According to the method, 2,3-epoxypropyltrimethylammonium chloride (GTMAC) is grafted onto chitosan to obtain a quaternized chitosan polymer (QCS) as the main raw material of a hydrogel, p-carboxybenzaldehyde is grafted onto a triblock copolymer Pluronic< >F127 to obtain an aldehyde group-terminated Pluronic< >F127 polymer (PF127-CHO) as the cross-linking agent of the gel, and the aldehyde group-terminated Pluronic< >F127 polymer (PF127-CHO) and the quaternized chitosan polymer (QCS) are subjected to a cross-linking reaction under a physiological environment to obtain the antibacterial adhesion injectable hydrogel dressing (QCS / PF) with advantages of rapid self-healing, easy extending and easy compression. According to the present invention, the method has advantages of simple process, wide raw material source and low preparation cost; and the hydrogel dressing prepared by using the method has advantages of good adhesion, good pH responsiveness, good mechanical property, good self-healing property, good bleeding stopping property and good antibacterial property so as to be used in the field of skin injury treatment drugs.

The invention provides a quaternized chitosan modified bentonite adsorption material. Chitosan for sodium based bentonite is modified at first, chitosan macromolecules are compounded with the bentonite, on the one hand, characteristics of high expansion, high suspension and the like of the bentonite in the waste water can be reduced, on the other hand, the problem that the chitosan is small in density, floats easily and is difficultly in contact with pollutants in a solution is solved; the chitosan sodium based bentonite is subjected to intercalation modification by using (2,3-glycidyl) trimethylammonium chloride as a modifying agent to obtain the quaternized chitosan modified bentonite adsorption material with a layered hole structure, the bentonite adsorption material has large specific surface area and has good adsorption property on the lead ions in the waste water, the maximum adsorption amount can reach 91.81 mg / g, and therefore, the quaternized chitosan modified bentonite adsorption material conforms to a Langmuir isothermal adsorption model and a quasi-second-order kinetic model, belongs to monomolecular-layer adsorption and is chemical adsorption.

The invention relates to a method for producing a quaternary ammonium chitosan / polyving akohol membrane, comprising the following steps: (1) synthesizing the quaternary ammonium chitosan; (2) washing and drying the quaternary ammonium chitosan; (3) synthesizing the quaternary ammonium chitosan / polyving akohol membrane; and (4) scraping the quaternary ammonium chitosan / polyving akohol membrane on a flat plate and forming the quaternary ammonium chitosan / polyving akohol membrane. Compared with the prior art, the method for producing the quaternary ammonium chitosan / polyving akohol membrane adopts the raw materials of rich source and low cost, is easy and is convenient to operate.

The invention discloses a method for preparing a polymer membrane, in particular to a method for preparing a quaternized chitosan modified polysulfone ultrafiltration membrane. The method includes jointly putting polysulfone and anhydrous zinc chloride in an N-methyl pyrrolidone, stirring and dissolving to obtain a uniform solution A; putting quaternized chitosan in dimethyl sulfoxide, stirring and dissolving to obtain a uniform solution B; adopting a solution mixing method to mix the uniform solution A with the uniform solution B to obtain a uniform solution, namely a needed membrane casting solution; subjecting the mixed solution to vacuum defoaming for 2 hours, standing for 2 hours, uniformly coating the mixed solution on polyester non-woven cloth, and using a scraper to scrape a liquid membrane to be a membrane uniform in thickness; soaking the membrane in pure water for a phase inversion reaction to obtain the polysulfone ultrafiltration membrane. The method can be widely applied to enterprises of special separation.

The invention belongs to the technical field of medical materials, and discloses a moisture-absorbing antibacterial wound dressing. The dressing comprises quaternized chitosan fiber fabric. The substitution degree of quaternized chitosan fibers is 0.1-1.0. The quaternized chitosan fibers are prepared through the following steps that 1, a quaternization reagent is dissolved in anhydrous alcohol or propyl alcohol to obtain a quaternization solution; 2, chitosan fibers are soaked in the quaternization solution and reacted for 1-10 h at 50-100 DEG C; 3, after the reaction is finished, washing and drying are performed to obtain the quaternized chitosan fibers. According to the moisture-absorbing antibacterial wound dressing, while antibacterial performance is improved, moisture absorption is improved, and high wet strength is maintained.

The invention discloses a multifunctional temperature-sensitive gel composite dressing for wound healing and preparation and usage thereof. The dressing comprises a quaternized chitosan derivative and an aldehyde-terminated temperature-sensitive polymer, a mass ratio of the two components is 1:15-1:25, wherein a molecular weight of chitosan for preparing quaternized chitosan is between 100 thousands and 1000 thousands, the degree of deacetylation is above 90%, and the degree of quaternized substitution is 10%-45%. The dressing is a thick liquid easy to apply to a wound at room temperature, and after it is applied to the wound, a gel film coating may form within 1 min. The temperature-sensitive gel composite dressing prepared based on the invention has autonomous antibacterial and anti-inflammatory effects and good film-forming stability, may also provide a wet environment and a sustained drug release function and is useful for treating and healing wounds such as diabetic foot ulcers.

A quaternary ammonium chitosan derivative able to be used as solubilizer of the medicines difficult to dissolve, its preparing process and its composite medicine are disclosed.

The invention discloses a quaternized chitosan / polyacrylic acid composite nanofiltration membrane and a preparation method thereof. The preparation method comprises the following steps: by a layer-by-layer deposition method, taking positively-charged 2-hydroxypropyl trimethylammonium chloride chitosan as a cationic polymer and polyacrylic acid as an anionic polymer, then introducing a NaCl solution as a supporting salt, alternately depositing, self-assembling layer by layer to form a composite membrane, and finally crosslinking in epoxy chloropropane to obtain the composite nanofiltration membrane. The preparation method provided by the invention is simple in process, natural and environment-friendly; the prepared positively-charged composite nanofiltration membrane has good hydrophilicity, excellent mechanical properties and an excellent swelling property, has the purified water permeation coefficient of 19.29L.h<-1>.m<-2>.Mpa and the cutoff molecular weight of 989, and can be used for separating and removing micro-trace pollutants, especially the positively-charged substances, in a water environment.

The invention relates to a synthesis method of similar quaternized chitosan sunscreen and an application of the quaternized chitosan sunscreen as hair sunscreen, belonging to the technical field of functional cosmetic. The product in the invention is obtained through the following two reaction steps: first step, 2-hydroxypropyltrimethyl ammonium chloride chitosan is synthesized from 3-chloro-2-hydroypropyltrimethyl amino and chitosan under alkali condition; second step, 2-hydroxypropyltrimethyl ammonium chloride chitosan is dissolved in dimethyl sulfoxide, and acyl chloride (sulfonyl chloride) and bind acid agent triethylamine are added in the solution to synthesize the final product quaternized chitosan sunscreen. Wherein, the acyl chloride (sulfonyl chloride) group grafted in the second step has quite strong ultraviolet absorption performance; besides, grafting quaternary ammonium group on the chitosan not only improves the water solubility of the chitosan and enlarges the application scope, but also enables the product to adhere to hairs with negative electricity. Measurement of tryptophan content in hairs proves that the product has sun-screening effect for hairs to some extent.

The invention discloses a method for preparing an antibacterial agent by immobilizing Chinese herbal medicines with attapulgite. The method comprises the following steps: spraying Chinese herbal medicine active components on the surface of attapulgite powder while stirring, mechanically grinding, sequentially spraying an aqueous solution of liquid fatty glyceride and quaternized chitosan oligosaccharide, stirring for uniform mixing, and rolling, drying, crushing and packaging the obtained mixture. The Chinese herbal medicine active components with antibacterial activity reside in an attapulgite pore channel structure through mechanochemical action, the fatty glyceride is used for forming primary encapsulation on the surface of attapulgite, secondary encapsulation is achieved through quaternized chitosan oligosaccharide, the obtained attapulgite immobilized Chinese herbal medicine antibacterial agent has good thermal stability, and the defect that Chinese herbal medicine antibacterial active components are poor in thermal stability is overcome.

The invention discloses a method for preparing a natural polyelectrolyte nanofiltration membrane and belongs to the technical field of preparation of membrane materials. According to the method disclosed by the invention, a quaternary ammonium cation cellulose ether and a quaternized chitosan blend form an ultra-thin separation layer material, polyvinyl alcohol serves as a casting solution additive, and polysulfone serves as a supporting layer material; two kinds of natural polyelectrolyte serve as a membrane material, and a cross-linking agent is added, so that the two polymers are cross-linked to form a spatial semi-interpenetrating network structure, the polymer networks are mutually tangled, and therefore, the membrane structure stability is enhanced. Meanwhile, the natural polyelectrolyte charged nanofiltration membrane has an antibacterial group capable of inhibiting and killing bacterium components in water, while the membrane structure is provided with many hydrophilic groups,and the hydrophilic property on the membrane surface is excellent. Meanwhile, the raw materials are readily available, the cellulose belongs to renewable resources, the chitosan belongs to natural polysaccharides, any risk of environmental pollution is avoided, and the preparation method is convenient and practical.

The invention discloses a quaternized chitosan containing wet wipe compound liquid with the functions of anti-bacteria, moisture retention and skin protection and a preparation method thereof. The quaternized chitosan containing wet wipe compound liquid comprises the following components in percentage by weight: 0.01%-1% of cation water-solubility chitosan derivatives, 0.3%-0.6% of preservative, 0.05%-5% of bactericide, 0.5-3% of wetting agent, 5-20% of skin-protecting agent and the rest of deionized water. The quaternized chitosan containing wet wipe compound liquid has the functions of anti-bacteria, moisture retention and skin protection, is good in water-solubility, convenient to use and environment-friendly; and the skin feel of the common wet wipe compound liquid can be obviously increased.

The present invention belongs to the field of polymer compound material. The invention discloses an O-quaternary-N-thiourea chitosan, a preparation method and application thereof, and the chitosan is a chemical modified functional derivative of chitosan. The preparation method of the O-quaternary-N-thiourea chitosan comprises the following steps: adopting chitosan as a raw material and carrying out reaction with benzaldehyde to obtain chitosan Schiff base, then carrying out reaction with 2,3-glycidyl trimethyl ammonium chloride to obtain O-quaternary-N-chitosan Schiff base; carrying out deprotection of an amino group positioned on the second carbon atom (C2) of the O-quaternary-N-chitosan Schiff base through a methanol-hydrochloric acid solution to obtain O-quaternary chitosan, adding the O-quaternary chitosan to the mixture of ammonium thiocyanate and chloroacetyl chloride to carry out reaction, and then obtaining the O-quaternary-N-thiourea chitosan. The O-quaternary-N-thiourea chitosan provided in the invention achieves a substantial increase in antibacterial property compared with chitosan. The invention can be used in a plurality of application fields of antibacterial material, daily chemical products, industrial wastewater treatment and the like.

The invention relates to a polyvinyl alcohol / collagen / quaternized chitosan electrospun composite fiber film and a preparing method thereof, and belongs to the field of medical devices. The method includes firstly evaluating morphology of spinning fibers through adjusting a mass ratio between polyvinyl alcohol and collagen and concentrations so as to determine that the optimum ratio between the polyvinyl alcohol and the collagen is 80:20, and that the optimum concentration is 8%; and then when the concentration of added quaternized chitosan is 5%, preparing the composite fiber film by an electrospinning technique. The composite fiber film is simple and convenient to prepare, has good flexibility and mechanical properties, can rapidly stop bleeding, and can form a protective fiber felt layeron wound surfaces to promote wound healing and prevent bacterial infection, thus healing skin wounds. The composite fiber film also has a bleeding arresting function, a wound healing function, antibacterial and anti-inflammation functions, and the like, is suitable for use as a bleeding arresting material, a wound healing material, and a medical device material, and has a good application prospect.

The invention relates to a chitosan derivative antibacterial superabsorbent gel and a preparation method therefor. The gel is prepared by using quaternized chitosan, acrylic acid, an initiator and a crosslinking agent, and mass percentages of raw materials are: 10-25% of quaternized chitosan, 75-90% of acrylic acid, 0.5-3% of initiator and 0.2-0.6% of crosslinking agent. The degree of swelling of the superabsorbent gel in water can reach 500 (g / g), and the gel has good antibacterial properties for gram-negative and gram-positive bacteria. The preparation method is simple in process and low in cost.

The invention discloses a method for preparing a carvacrol microcapsule antibacterial agent from attapulgite stabilized oil-in-water emulsion. The method comprises the following steps: firstly, preparing the attapulgite stabilized oil-in-water emulsion from antibacterial agent carvacrol molecules under the action of an emulsifying agent on the basis of water solubility and cationic property of quaternized chitosan oligosaccharide and in combination with biological activity and nanometer effect of natural nano-material attapulgite; then, encapsulating the natural antibacterial agent carvacrol with volatile and irritating odor with a microcapsule preparation technology to prevent volatilization of carvacrol; further forming a protective shell layer by cross-linking with calcium chloride; finally, obtaining the carvacrol microcapsule antibacterial agent by separation and drying. The carvacrol microcapsule antibacterial agent has broad-spectrum antibacterial activity and high antibacterialefficiency, is free of irritating smell, also has high thermal stability and antibacterial durability, can be applied to feed additives, and solves the current situation of long-term dependence on antibiotics in the animal breeding process in China.

The invention discloses quaternized chitosan / siRNA (small interfering Ribonucleic Acid) composite particles and a preparation method thereof. Electrostatic interaction between a quaternary ammonium group with positive charges and a siRNA molecule with negative charges on quaternized chitosan is used as driving power; molecule self-assembly is initiated in a water phase through vortex oscillation; and the high-bioactivity quaternized chitosan / siRNA composite particles with controllable size and surface charge are prepared by adjusting and controlling parameters such as molecular charge ratio, incubation temperature and incubation time. According to the quaternized chitosan / siRNA composite particles and the preparation method thereof, the quaternized chitosan is used as a siRNA transfer carrier, and the material has low cytotoxicity, wide source and low cost; a preparation process of the composite particles has mild conditions and is simple and practical; and the bioactivity of the siRNA can be effectively kept. The siRNA used for preparing the composite particles can be effectively intaken by cells and a mum effect of the target gene can be induced aiming at specific design of a target gene; and the composite particles have good application prospect in the fields of cancer treatment, tissue regeneration and repair and the like.

The invention discloses a quaternary ammonium chitosan-polycaprolactone grafted copolymer and a preparation method thereof as well as a reticular membrane prepared by the copolymer and a method thereof, wherein, the polycaprolactone branched chain of the uaternary ammonium chitosan-polycaprolactone grafted copolymer is grafted on the chitosan C-6 position, the 2-hydroxy-3-methyl amine propyl chloride side chain is connected to the chitosan C-2-position, and the obtained grafted copolymer material is loaded with selected drugs by a method of solution mixing and assembled into a gradient-structured reticular membrane by using the electro-wet spinning technology. The membrane has the advantages of good mechanical properties, adjustable pore structure, bio-degradability, moisture retention and air permeability, anti-bacterial and bactericidal capacity and drug controlled release capacity. The process of material synthesizing and processing and preparation for the membrane is simple, the grafted copolymer material structure and the membrane structure are adjustable, the drug release rate is controllable, and the adopted raw materials are mainly natural biological material and have rich sources. The membrane can be used in the treatment of different degrees of skin injury and in the tissue engineering scaffolds for follow-up skin tissue repair.

The invention discloses a preparation method of a positively charged graphene oxide / quaternized chitosan composite nanofiltration membrane prepared through layer-by-layer self-assembly. The method comprises the step that the positively charged graphene oxide / quaternized chitosan composite nanofiltration membrane is prepared through a layer-by-layer self-assembly method by taking positively charged 2-hydroxypropyltrimethyl ammonium chloride chitosan as a polycation electrolyte, taking graphene oxide as a polyanion electrolyte, taking a polyethersulfone ultrafiltration membrane which is good in hydrophilic performance as a supporting layer and taking epichlorohydrin as a cross-linking agent. The preparation method is simple, natural, environmentally friendly and good in stability, the prepared positively charged composite nanofiltration membrane has the good hydrophilic performance and the excellent separating effect, the pure water permeability coefficient is 24.02 L.h<-1>.m<-2>.Mpa, the molecular weight cut-off is 1,313, and the molecular weight cut-off of the nanofiltration membrane ranges from 150 to 2,000, and the prepared positively charged composite nanofiltration membrane belongs to the nanofiltration membrane category. The composite membrane has the high retention rate on high-valence cation compounds and can be used for treatment of micro-balanced pollutants in a water environment and particularly separating and removing of positively charged materials.

The invention relates to the technical field of fuel cells, in particular to a preparation method of a quaternized chitosan anion hybrid membrane doped with positive polystyrene latex. The preparation method comprises the following steps of: (1) preparing the positive polystyrene latex; (2) preparing quaternized chitosan; (3) dissolving the quaternized chitosan powder into acetic acid solution with the concentration being 2wt%, then adding the prepared positive polystyrene latex, forming membrane casting liquid, stirring the membrane casting liquid for 4 hours under the room temperature, and carrying out ultrasonic treatment for 20-40 minutes; then adding glutaraldehyde at the speed of 0.5mLmin<-1> with the adding amount accounting for 0.1-0.3% of the total volume of the membrane casting liquid, stirring for 1 hour, then carrying out ultrasonic treatment for 30 minutes, finally pouring the solution subjected to ultrasonic treatment on a horizontal glass plate, and evaporating a solvent at the temperature of 40 DEG C to form the hybrid membrane; and (4) carrying out after treatment on the hybrid membrane. According to the preparation method, the ion exchange capacity and the electric conductivity of the hybrid membrane are high, and the methanol penetration rate is low.

A method for preparing a nanofiltration membrane used for removing divalent cation and PPCPs with positive charges is disclosed. The PEI / TMC / HTCC composite nanofiltration membrane is prepared by adopting polyethersulfone nanofiltration membrane as a base membrane, and adopting twice interfacial polymerization, with polyethyleneimine and quaternized chitosan being adopted as water-phase monomers offirst and second times of interfacial polymerization respectively and 1,3,5-trimesoyl chloride being adopted as an organic-phase monomer. The inorganic salt trapping priority of the composite membrane is in this order: MgCl2, CaCl2, MgSO4, Na2SO4, NaCl and KCl. The trapping rates for MgCl2 and Na2SO4 are respectively 90.48% and 78.13%, and the composite membrane shows a high removing rate for a high-valence cation salt solution. The trapping priority of the composite membrane for carbamazepine, ibuprofen, atenolol and amlodipine which are four different PPCPs is in this order: AML, ATE, CBZ and IBU, the trapping rate for AML with positive charges is 90.12%, and the trapping rate for PPCPs with positive charges is higher than trapping rates for PPCPs with positive charges and electric-neutral PPCPs. The method is simple, and low in cost. The prepared composite nanofiltration membrane has a pure water permeability coefficient of 25.37 L.m<-2>.h<-1>MPa<-1>, the molecular weight cut-off is 481 Da, and the composite nanofiltration membrane has good hydrophilicity and fouling resistance.

The invention discloses a preparation method of an organic-inorganic composite attapulgite mycotoxin adsorbent. The preparation method comprises: modifying acid activated attapulgite by using quaternized chitosan oligosaccharide, carrying out a reaction on the obtained material and acid activated active carbon, carrying out centrifugation, washing, drying, and crushing to obtain the organic-inorganic composite attapulgite mould toxin adsorbent. According to the present invention, the obtained product can effectively adsorb and remove all kinds of mycotoxins in the feed, has antibacterial function, and can reduce the antibiotic consumption in the feed.

The present invention relates to a topical anti-microbial composition and method of use thereof. In particular, the present invention relates to a topical anti-microbial composition comprising a quaternized chitosan derivative having a polymerizable organic moiety and an amphoteric surfactant.

The invention relates to a method for improving fabric ink-jet printing performance by using quaternary ammonium chitosan, belonging to the technical field of textile chemistry. The method is characterized by comprising the following steps of: respectively using dipping or padding manners to pre-process the fabric by using the water-soluble quaternary ammonium chitosan; then drying and jetting ink and printing. The improved pre-processing technique of the quaternary ammonium chitosan can obviously increase the ink-jet printing performance of the fabric, improve the fineness and color depth of the printing fabric, and increase printing color firming degree. Furthermore, the fabric has good hand feeling and antibacterial performance.

The invention discloses a preparation method of a quaternary ammonium chitosan HTCC-silver / PES antibacterial film. First, the positively charged HTCC is used as the surface active functional layer, the PES ultrafiltration membrane with good hydrophilic performance is used as the support layer, and epichlorohydrin is used as the crosslinking agent, and the positively charged quaternary ammonium is prepared by coating crosslinking reaction method. Chitosan HTCC / PES composite nanofiltration membrane. Then soak the nanofiltration membrane in a certain concentration of silver nitrate solution, and dry the membrane with a nitrogen blower. Finally, photochemical reduction technology is used to reduce the silver ions on the membrane surface into silver particles. The preparation method has a simple process, can be carried out at normal temperature, has good reproducibility, does not need to add a reducing agent, is easy to control the particle size, and is natural and environmentally friendly. The antibacterial film prepared according to this method has excellent hydrophilic properties, mechanical properties and swelling properties, and is resistant to Gram-negative bacteria E. coli Rosetta and E. coli DH5α and Gram-positive bacteria Bacillus subtilis Both have good antibacterial ability, and this kind of membrane has a high rejection rate for most PPCPs, and can be used for the separation and removal of trace-trace pollutants in the water environment, especially positively charged substances.

The invention relates to a nano-antibacterial coating preparation method based on quaternized chitosan half-bred micelles. The preparation method includes the steps that firstly, quaternized chitosan is dissolved in 1% glacial acetic acid water solution to obtain a quaternized chitosan solution, then L-cysteine is dissolved in water to prepare an L-cysteine water solution, the two prepared solutions are mixed, a certain amount of solid nanozinc oxide is added, continuous stirring is performed, a pH value of the solution is regulated to promote system self-assembly after even mixing, and a half-bred micelle solution is obtained; finally, the half-bred micelle solution is used as electrolytic solution, the half-bred micelles are fixed to the medical metal surface by combining with an electrophoretic deposition technology to form a nano antibacterial coating. The nano-antibacterial coating preparation method is simple and convenient to operate, low in cost and wide in application range.

The invention discloses enteric nano-microparticles loaded with insulin as well as a preparation method and applications of the enteric nano-microparticles. The nano-microparticles are composed of nanoparticles obtained by compounding quaternization chitosan, insulin and sodium tripolyphosphate through the electrostatic interaction, and eudragit coating the surfaces of the nanoparticles. The compound nanoparticles loaded with insulin and having positive charges on the surface are prepared first by utilizing the FNC technology, and then eudragit coats the surfaces of the compound nanoparticles;the preparation method has the advantages that the continuous preparation is realized, the controllability is high, the batch repeatability is high, etc., and the obtained medicine-carrying particleshave high medicine loading capacity and high encapsulation efficiency. The enteric material coats the surfaces of the particles, after oral medication of the medicine-carrying particles, the loaded protein medicine can be effectively protected against the degradation of gastric acid and gastrointestinal tract protease, and thus the oral medication absorption efficiency of insulin at the small intestine part is improved. Compared with the subcutaneous injection, the enteric nano-microparticles loaded with insulin have the advantages that after oral medication, the good and stable effect of reducing blood glucose is shown, the high oral medication bioavailability is also obtained, and thus the application prospect is great.

The invention relates to a method for preparing quaternized chitosan nano iodine disinfectant, and hydroxy or amino of chitosan or chitosan with low molecular weight are respectively connected with quaternary ammonium salt monomers, and a quaternized chitosan derivative or a quaternized chitosan derivative with low molecular weight is prepared, and the original biological activity of chitosan is still maintained in the derivative, and simultaneously solubilities of the product in water and other solvents are increased; the quaternized chitosan derivative or quaternized chitosan derivative with low molecular weight are reacted with iodine for generating chitosan group nano iodine compound or low molecular weight chitosan group nano iodine compound, and the chitosan group nano iodine disinfectant or low molecular weight chitosan group nano iodine disinfectant is prepared by dissolving in solvent. Compared with the prior art, the iodine has good dispersibility and stability in the invention, and because of surface effect and subsize effect of chitosan group nano iodine, bacteriostasis, antiviral activity and continuity of the product are improved, and effective utilization rate of iodine is increased.

The invention discloses a preparation method of a trinary composite stent of plasmid DNA / fibrin gel / polymer. The preparation method comprises the following steps of: geometrically and uniformly mixing an N-trimethyl chitosan solution and a plasmid DNA solution to prepare plasmid DNA composite particles; dissolving fibrinogen in physiological saline to prepare a fibrinogen solution; dissolving thrombin in a calcium chloride solution to prepare a thrombin calcium chloride solution; sterilizing a polymer porous stent with ethanol; simultaneously filling the plasmid DNA composite particles, the fibrinogen solution and the thrombin calcium chloride solution with the same volumes in the polymer porous stent by utilizing negative pressure; and arranging the stent filled with the plasmid DNA composite particles, the fibrinogen solution and the thrombin calcium chloride solution in a constant-temperature oven to fully gelatinize the fiber protein. The preparation method is simple and convenient, uses materials with wide resources and can simulate the composition and the structure of the cartilage of a human body. The composite stent of the invention has favorable mechanical property and biological activity, can accelerate the restoration of the cartilage and the osseous tissue and have strong application values of cartilage and bone restoration.