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Low-oxygen culture method for akkermansia muciniphila

A culturing method and a mucin technology, which are applied in the field of culturing Akkermansia muciniphila, can solve the problems of fluctuating gas atmosphere, unreachable technology, affecting the growth of Akk bacteria, etc. The effect of shortening time

Inactive Publication Date: 2019-12-10
君维安(武汉)生命科技有限公司
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Problems solved by technology

Yet, only studied the oxygen concentration (0.1%) when Akk bacterium is in the stationary phase (thestationary phase) in this article, and the hypoxic culture atmosphere of the most vigorous logarithmic phase of Akk bacterium growth is not done research; Adopt this paper The oxygen level in the medium, Akk bacteria need to be cultivated for a long time to enter the stabilizer, and for the research of Akk bacteria, it is necessary to make it grow as soon as possible, and quickly reach the stable period, and more Akk bacteria have been obtained. Obviously, the technology of this paper The above effects cannot be achieved, and the cultivation efficiency of Akk bacteria cannot be improved; in addition, the 0.1% oxygen level requirement is still relatively strict for the cultivation of Akk bacteria, and the gas atmosphere is prone to fluctuations during cultivation, which affects the growth of Akk bacteria

Method used

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  • Low-oxygen culture method for akkermansia muciniphila

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Experimental program
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Embodiment 4

[0023] Example 4 uses medium I, selected from the reference (Derrien M.Akkermansia muciniphilagen.nov.sp.nov.a human intestinal mucin-degrading bacterium[J].Int.J.Syst.Evol.Microbiol.2004,54. ) the same mucin medium.

Embodiment 1～3 and comparative example 1～3

[0025] The cultivation method of the Akk bacteria adopted in this embodiment is as follows: the Akk bacteria were activated for 48 hours, inoculated into the BHI culture medium at an inoculum size of 1%, and cultivated at a constant temperature of 37°C; the volume ratio of the mixed gas is shown in Table 1 below.

[0026] Table 1 embodiment 1~3 and comparative example 1~3 adopt the mixed gas composition and the volume ratio of A culture medium

[0027] N 2

O 2

CO 2

Example 1 88％ 2％ 10％ Example 2 70％ 5％ 25％ Example 3 94％ 1％ 5％ Comparative example 1 90％ 0 10％ Comparative example 2 90％ 0.1％ 9.9％ Comparative example 3 68％ 7％ 25％

[0028] Example 4

[0029] The cultivation method of the Akk bacteria adopted in the present embodiment is as follows: the Akk bacteria were activated for 48 hours, inoculated into medium I at an inoculum size of 1%, and cultivated at a constant temperature of 37°C; the volume rat...

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Abstract

The invention discloses a low-oxygen culture method for akkermansia muciniphila. In the low-oxygen culture method, a low-oxygen gas environment is mainly created; a volume ratio of various componentsof mixed gas is as follows: 70-94 percent of N2, 1-5 percent of O2 and 5-25 percent of CO2; and the culture time is 24-36h. The invention provides a brand-new low-oxygen mixed gas atmosphere which isrelatively high in oxygen concentration and more suitable for growth of Akk bacteria. Compared with a traditional absolute oxygen-free environment and an existing micro-oxygen environment, by adoptingthe method disclosed by the invention, the time of Akk bacterium culture, entering a stabilization period, is greatly shortened, and the low-oxygen environment can also show a similar promotion effect for different culture media.

Description

technical field [0001] The invention belongs to the technical field of cultivation of Akkermansia muciniphila, in particular to a low-oxygen cultivation method for Akkermansia muciniphila. Background technique [0002] Akkermansia muciniphila (Akkermansia muciniphila) is the first intestinal anaerobic bacterium isolated in Verrucomicrobial phylum, which was successfully isolated in 2004. The latest research found that Akkermansia muciniphila (hereinafter referred to as Akk bacteria) of the genus Akkermansia may be one of the important marker microorganisms to measure the balance of intestinal microecology. Akkbacterium is a functional bacterium with the characteristics of regulating blood sugar homeostasis, which widely exists in the intestinal tract of healthy people. It has the property of degrading mucin and is an important microorganism living on the mucus surface of the host intestinal mucosa. Studies in recent years have shown that Akk bacteria are related to many me...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/01
CPCC12N1/20
Inventor 王敏石长萍
Owner 君维安(武汉)生命科技有限公司
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