33 results about "Desulfovibrio" patented technology

The invention provides for methods of treating autism associated with Desulfovibrio overgrowth in the gastrointestinal tract of a patient, said method comprising administering to the patient suffering from said autism a treatment course of aztreonam in an amount effective to treat autism in the patient, thereby treating autism.

The invention provides for methods of treating autism associated with Desulfovibrio overgrowth in the gastrointestinal tract of a patient, said method comprising administering to the patient suffering from said autism a treatment course of aztreonam in an amount effective to treat autism in the patient, thereby treating autism.

A dietary supplement that may contain probiotics from the genera Akkermansia, Bacteriodes, Faecalibacterium, Eubacterium, Escherichia, Collinsella, Desulfovibrio, Clostridium, Mycobacterium, Pediococcus, and Bifidobacterium. The dietary supplement may provide a variety of benefits including weight management, blood sugar management, treatment of irritable bowel syndrome, treatment of Crohn's disease, treatment of diverticulitis, treatment for inflammatory bowel, treatment for dysbiosis, and strengthening the immune system.

Biomarkers for diabetes and usages thereof are provided. And the biomarkers are Akkermansia muciniphilacBacteroides intestinaliscBacteroides sp. 20_3cClostridium bolteaec Clostridium hathewayicClostridium ramosumcClostridium sp. HGF2cClostridium symbiosumc5 Desulfovibrio sp. 3_1_syn3cEggerthella lentacEscherichia colicClostridiales sp. SS3 / 4c Eubacterium rectalecFaecalibacterium prausnitziicHaemophilus parainfluenzaecRoseburia intestinalis and Roseburia inulinivorans.

The invention discloses fertilizer for Cr (VI) polluted soil remediation and improvement and a preparation method of the fertilizer. The preparation method of the fertilizer comprises the following steps: spraying an EM microbial agent to the surfaces of bagasse and pig manures, then uniformly mixing the bagasse with the pig manures, and composting the mixture to obtain a cured material; adding anOneida silva microbial agent, the EM microbial agent and a desulfovibrio microbial agent into the cured material, uniformly mixing the microbial agents with the cured material, and composting the mixture to obtain a microbial compound; and mixing ferrous sulfate, modified coal ash, ground calcium carbonate and the microbial compound, and granulating the mixture to obtain the fertilizer. The fertilizer can improve the soil structure, improve the soil fertility and remediate and improve a microecological structure of the soil when effectively remediating chromium pollution, finally remetiates and improves the chromium-contaminated soil, and restores the ecological functions.

The invention discloses a method for preparing a strain and a microbial agent for heavy metal contaminated water treatment. The strain is a sulfate reducing bacterium Desulfovibrio oxamicusw, has a preservation number of CGMCC 9548, and is named D. oxamicusw CGMCC 9548. The D. oxamicusw CGMCC 9548 has a removal rate on SO4<2->, Pb, Cd, As and W to be over 80 percent, so that heavy metals in a water body can be removed, and the pH value of the water body can be increased. The strain is applied to heavy metal water contamination treatment of industrial and acid mine wastewater. The method provides a developed fermentation method to large-scale fermentation production of D. oxamicusw CGMCC 9548 strain microbial agent, and the product strain density is over 1012. The microbial agent can be used for removing heavy metals in a water body and increasing the pH value of the water body, and can be applied to heavy metal water contamination treatment of industrial and acid mine wastewater.

The invention relates to a novel microorganism flora mixture and a mixed nutrient medium thereof. The nutrient medium is filter liquor which is prepared by taking plant powder, mineral composition, animal powder, organic acid and the like as raw materials, adding sewage or comprehensive sewage in cities to be processed, mixing, fermenting and filtering. The microorganism flora mixture is a microorganism preparation which is prepared by the following steps: respectively selecting fusarium oxysporum, aspergillus fumigatus, sphaerotilus, vorticella microstoma, photosynthetic bacteria, methanebacteria, desulphovibrio, nitrobacterium, pseudomonas fluorescens and other bacterial strains from obligate aerobes group, anaerobic bacteria group and facultative aerobe group preferentially, then expanding and cultivating, inoculating into the above mixed nutrient medium, mixing, cultivating and domesticating for 15-30 days at the temperature of 20-45 DEG C. The mixed nutrient medium in the invention can cultivate, propagate and domesticate flora mixture with the combination advantages of flora; the flora has stable integrity, no easy variation and strong adaptability, and the invention breaks through the technical bottlenecks of easy degeneracy of microorganism flora, easy decrease of biological activity and slow proliferation and expansion.

The invention discloses an eco-friendly method for in-situ rapid biological repair for the heavy metal pollution of soil, and an application. The technology belongs to the field of environment protection. The technology is realized through the following steps: adding an appropriate amount of plant straw, green Chinese onion straw and / or garlic straw and rape seed cake in the soil with the heavy metal pollution, turning over, adding an appropriate amount of desulfovibrio, desulfuromonas, desulfobulbus and the like, repeatedly turning over the soil and uniformly mixing the soil with the additives, watering, covering the soil with a plastic film for sealing, thoroughly turning over the soil every 5-7 days, and continuously turning over the soil for 6-8 times, and in this way, most of heavy metals in the polluted soil can be solidified-precipitated and passivated, and the bioactivity and solubility of the heavy metals are greatly reduced, so that the heavy metal pollution of the soil and the poisoning of the heavy metal pollution are eliminated, and environment is protected.

In order to solve the problem that conventional soil remediation technology has a single remediation function, can only remedy soil hardening and salinization and cannot reduce the content of heavy metal, the invention provides a microbial soil remediation agent and a soil remediation method. The microbial soil remediation agent is composed of the following substances, by mass: 1-3 parts of bacillus subtilis, 1-5 parts of acidithiobacillus ferooxidans, 1-2 parts of cyanobacteria, 1-2 parts of sulfate reducing bacteria, 1-2 parts of desulfovibrio desulfuricans, 1-2 parts of trichoderma, 2-4 parts of penicillium spinulosum, 2-5 parts of bacillus coagulans, 3-5 parts of rhodopseudomonas palustris, 2-5 parts of garcinococci, and 2-5 parts of cold salt tolerant gingivalis. The microbial soil remediation agent and the soil remediation method of the invention can effectively reduce the heavy metal content of oil, adjust the pH of the soil, reduce the cadmium content of crops and increase theyield of the crops.

The invention relates to a synergistic biological treatment method for washing waste water and amine salt waste liquor produced by capturing CO2 in smoke of a coal-fired power plant. Waste amine salt in amine salt waste liquor produced by a regenerating tower in a flue gas CO2 capturing process is taken as one organic carbon source in a biological treatment process, an anaerobic and aerobic two-stage biotreatment is adopted for treating the washing waste water and the amine salt waste liquor, the washing waste water and the amine salt waste liquor are pumped into an anaerobic biological reaction column by a water pump, nitrate is converted into N2 under the action of denitrifying bacteria, sulphate is converted into S<2-> under the action of Desulfovibrio, organic carbon in organic amine salt is taken as a partial organic carbon source for sulphate reduction bacteria, and amine is converted into ammonia nitrogen. Waste water after anaerobic treatment enters an aerobic biological reaction column, nitration and denitrification of ammonia nitrogen are realized by carrying out intermittent aeration by an air pump, and finally ammonia nitrogen is released into the air in the form of N2; S<2-> generates sulphur under the action of thiobacillus thioparus, simple substance sulphur is recycled by a sulphur separator, and separated waste liquor returns to a washing tower to be recycled; and no pollution is produced in a CO2 capturing process.

The invention relates to bifidobacterium longum subspecies CCFM687, fermented food and application thereof. Bifidobacterium longum infantile subspecies CCFM687 of the present invention can improve the depression-like behavior of depressed mice, improve the levels of 5-hydroxytryptamine, 5-hydroxytryptophan and brain-derived neurotrophic factor in the brain of depressed mice; improve depression Butyric acid content in the gut of mice; lower intestinal abundance of Desulfovibrio spp., increased abundance of Bifidobacterium and S24‑7 families, increased gut microbiota α‑diversity, and improved intestinal Intestinal flora disorder, reduce the occurrence of autism, inflammatory bowel disease, obesity and type I diabetes; increase the mRNA level of tryptophan hydroxylase 1 in simulated enterochromaffin cells, and increase the 5-hydroxychromatin level of the cells The secretion of amino acid provides precursor substances for the synthesis of 5-hydroxytryptamine in the brain. Therefore, it has broad application value.

The invention discloses a biological ferrous sulfide composite material which comprises ferrous sulfide and synthetic bacteria bonded to the ferrous sulfide .The ratio of the number of microorganisms of the synthetic bacteria to the ferrous sulfate of unit mass is 3.3*107-5*107 cfu / mg .The synthetic bacteria mainly comprise Desulfovibrio and Clostridium .The invention further discloses a uranium-polluted underground water treatment permeation reaction wall based on the biological ferrous sulfide .The uranium-polluted underground water treatment permeation reaction wall comprises a reaction well and a reaction medium .The reaction well is arranged in soil containing uranium-polluted underground water and located on an underground water flowing path, and the top of the reaction well is flush with the ground and provided with an upward first opening .The reaction medium is arranged in the reaction well and is the biological ferrous sulfide composite material .The prepared material and the device have high removal rate for uranium in water, secondary pollution is avoided, regenerability is good, and the requirements of current underground water uranium-polluted permeation reaction wall materials are met.

The invention discloses a composite bacterium, which can be used for the degradation of COD (Chemical Oxygen Demand) and / or organic sulfides and pretreatment for the degradation of garlic processing wastewater. The composite bacterium is obtained by mixing fermentation broth of bacillus megaterium, pseudomonas stutzeri, aspergillus oryzae, klebsiella oxytoca and desulfovibrio desulfuricans, of which bacterial cell concentrations are 20 to 30 percent, 10 to 15 percent, 15 to 35 percent, 10 to 35 percent and 5 to 15 percent respectively. The composite bacterium can be applied to the degradation of COD and / or organic sulfides, and can also be applied to the pretreatment for the degradation of the garlic processing wastewater. By such a biological pretreatment method for the degradation of the garlic processing wastewater, the influence of changes in the conditions of COD and organic sulfide concentrations, water volumes and the like on a degradation effect can be reduced, and use of a plurality of chemical reagents in a subsequent process can be reduced and even avoided; the pretreated wastewater can be discharged up to the standard in an activated sludge treatment system.

The invention discloses application of chitosan oligosaccharide as regulating agent for regulating animal intestinal flora disturbance, the chitosan oligosaccharide, a mixture containing the chitosanoligosaccharide and corresponding application. The polymerization degree of the chitosan oligosaccharide is 2 to 20, so that the intestinal flora disturbance caused by obesity or diabetes or an unreasonable diet structure can be regulated, growth of beneficial bacteria such as Akkermansia, Bifidobacterium and Lactobacillus of an intestinal tract is promoted, and proliferation of harmful bacteriasuch as Desulfovibrio and Helicobacter is obviously inhibited. The invention provides an application method of a clinically-acceptable medicinal preparation or health-care food which is made by usingthe chitosan oligosaccharide alone or a composition prepared from the chitosan oligosaccharide and the beneficial bacteria according to a conventional process. By utilizing the chitosan oligosaccharide, prevention and control of the intestinal disturbance caused by the diabetes, the obesity and the inflammatory bowel disease have an active application value.

The invention discloses a slow-release compound flocculant for reducing acidity of mine water. The slow-release compound flocculant is prepared from the following raw materials in percentage by weight: 87wt%-95wt% of an inorganic compound flocculant and 5wt%-13wt% of a soluble coating material, wherein the soluble coating material wraps the inorganic compound flocculant; the inorganic compound flocculant is a mixture of a microbial carbon source, a coagulant aid and an adsorbent; the soluble coating material is a culture medium mixed with a microbial agent; and the microbial agent is a mixed agent prepared from a desulfovibrio agent, a colourless sulfur bacteria agent and a gluconobacter agent. According to the slow-release compound flocculant, the characteristics of inorganic and biological flocculants are integrated; a chemical dosing mode is improved; the interaction efficiency of various flocculants is increased by multi-step dissolving and slow-release processes; and the efficiency of the agent is improved.

The invention aims to provide a microbial complex flora for eliminating sulfide in sediments. By the aid of the microbial complex flora, problems of narrow ecological niche, incapability of adapting to sediment environments, easiness in being sifting out within a short time, low biomass, slow metabolism and the like of pure bacteria can be solved. The microbial complex flora contains bacillus (Bacillus spp.), vibrio (Vibrio spp.), pseudomonas (Pseudomonas spp.) and desulfovibrio (Desulfovibrio spp.). The microbial complex flora has the advantages that bacteria of the microbial complex flora come from the sediments in inshore culture zones with high sulfide contents and are acquired by means of domestication, and accordingly adaptation procedures due to severe environment difference can be omitted during application; the excellent stability of the structure of the flora can be kept in sulfide removing procedures.

The present invention belongs to the field of biological technology, and relates to a kind of marine bacterium with strict araerobic activity, active extract of said bacterial strain culture and its preparation method, and the application value of said extract in the preparation of antibacterial medicine. Said invention provides a marine bacterial strain DM 18 obtained from the Huanghai sea of China by means of separation process, it is defined as culture fluid of Desulfovibrio desulfuricans. Its ethyl acetate extraction phase and n-butyl alcohol extraction phase are undergone the processes of silica-gel column chromatography and ceversal high-pressure liquid-phase separation so as to obtain two oil-like substances of effective component which have resistance for hay bacillus and staphylococcus aureus, and have potential application for preparing antibacterial medicine.

The invention relates to a method for treating chicken farm waste water by a desulfovibrio microbial fuel cell. The method includes the steps: activating and then inoculating desulfovibrio into culture solution, and culturing the closed desulfovibrio in an incubator at constant temperature until a last phase of a logarithmic phase; mixing the cultured bacterial solution, nutrient solution, buffersolution and sludge according to the volume ratio of (1-4):(1-4):(1-2):(4-7), inoculating mixture into an anode chamber of the microbial fuel cell, enabling the bacterial solution to be suspended in anode solution or adhered on an anode, standing to degrade the chicken farm waste water by the aid of the bacterial solution to generate electrons and protons, and enabling the electrons to reach a cathode through an external circuit and penetrate a middle diaphragm to reach protons of the cathode to form a current loop to generate power; updating anode solution once every 2-7 days, and retaining 10% of original mixture in the original anode chamber. The method has the advantages that the desulfovibrio is applied to microbial fuel cells based on treatment of chicken farm waste water, the degradation capability of the chicken farm waste water can be effectively improved, and electric energy is generated.

The invention relates to a method for treating bird waste water by a novel mixed bacterium microbial fuel cell. The method includes the steps: activating and then inoculating comamonas testosteroni and desulfovibrio into culture solution; culturing the closed comamonas testosteroni in a shaker, culturing the closed desulfovibrio in an incubator at constant temperature until a last phase of a logarithmic phase; uniformly mixing the cultured comamonas testosteroni and the cultured desulfovibrio, mixing mixture, nutrient solution, buffer solution and sludge according to the volume ratio of (1-3):(2-5):(1-3):(3-7), inoculating mixture into an anode chamber of the microbial fuel cell, enabling floras to be suspended in anode solution or adhered on an anode, and standing to degrade the bird waste water and generate power; updating anode solution once every 2-7 days, and retaining 10% of original mixture in the original anode chamber. The method has the advantages that mixed bacteria of the comamonas testosteroni and the desulfovibrio are applied to microbial fuel cells based on treatment of bird waste water, the degradation capability of the bird waste water can be effectively improved,and electric energy is generated.

The invention discloses an acidic soil conditioner; a microbial composite bacterial agent is prepared by mixing Desulfovibrio desulfuricans, Bacillus subtilis, Bacillus megaterium and Bacillus licheniformis according to a mass ratio of (3-10):1:1:1. The prepared microbial composite bacterial agent is mixed with plant ash, peat and nitrogen phosphorus potassium fertilizer at a mass ratio of (1-3):(50-100):(100-200):(7-12). The acidic soil conditioner is capable of improving activity of microbes in acidic soil and lowering the content of sulfate radicals in the acidic soil while adjusting pH of the acidic soil, comprehensive soil conditioning is achieved, and crop yield is increased.

The invention discloses a novel composite microbial system for degrading straw stalks as well as a preparation method and application thereof. The novel composite microbial system comprises bacteria such as Myroides, Ignatzschineria, Wohlfahrtiimonas, Alcaligenes, Bacteroides, Macellibacteroides, Escherichia, Arcobacter and Desulfovibrio. The method comprises the following steps: crushing live earthworms, adding the earthworms and collected microbial samples into a conical flask filled with a fresh selective medium for screening the composite microbial system; screening the selective medium with the highest degradation ability in the composite microbial system; determining strains in the composite microbial system through gene sequencing, and preparing a straw-decomposing inoculants to be applied to degrading the straw stalks. According to the synergistic effects among the strains, cellulose, hemicelluloses and lignin in the straw stalks can be synchronously degraded, and the degradation efficiency is high.

The invention provides a green sludge curing agent and application of the green sludge curing agent in sludge solidification, and belongs to the field of sludge treatment. The green sludge curing agent is prepared from the following components in parts by weight: 3-8 parts of microcrystalline cellulose, 30-50 parts of fly ash, 20-40 parts of calcium sulfate, 5-10 parts of silicate and 5-10 parts of spinel, wherein the microcrystalline cellulose is preferably organic-acid-anhydride-modified microcrystalline cellulose. The application of the green sludge curing agent in sludge solidification comprises the following steps that S1, microbial treatment is carried out, specifically, powdered sulfur , iron powder, a desulfovibrio medium and sludge are added into a container, desulfovibrio is inoculated under the anaerobic condition, anaerobic fermentation is carried out for 5-10 days to obtain fermented sludge; S2, sterilization is carried out, specifically, a fungicide is added to the fermented sludge, the mixture is stirred for 5-10 hours to obtain sterilized sludge; and S3, curing is carried out, specifically, the green sludge curing agent is added to the sterilized sludge, and the mixture is stirred evenly and stands still, that is, cured sludge is obtained. The green sludge curing agent has excellent mechanical performance, thermal stability and water resistance.

The invention provides a method for performing degradation on a pollutant-nitrobenzene-containing water body by using anaerobic mixed bacteria. The method comprises the following steps: mixing a catalyst, anaerobic sludge, an electron donor and a nitrobenzene-containing water body under anaerobic conditions to obtain a reaction solution; and carrying out oscillating reaction on the reaction solution under anaerobic conditions to obtain a treated water body. The catalyst is biochar of which the particle size is 80-120 meshes; and the anaerobic sludge comprises Pseudomonas aeruginosa, Marinospirillum, Desulfovibrio and Actinomycetes. The method provided by the invention adopts the biochar as the catalyst, and the anaerobic sludge is utilized to provide mixed bacteria and can accelerate the degradation of nitrobenzenes in the water body under anaerobic conditions. The method has high nitrobenzene degradation rate, and can not cause secondary pollution. The biochar is low in cost and easy to prepare. The method is simple and feasible in technical operation, and is convenient for practical popularization and use.

The invention provides a sulfate reducing bacterium and application thereof, the sulfate reducing bacterium is a desulfurization vibrio, is named as Desulfovibrio desulfuricans strain REO-01, is preserved in China General Microbiological Culture Collection Center on January 7, 2022, the address is No. 3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, and the strain preservation number is CGMCC NO.24267. The invention further provides a preparation method of the sulfate reducing bacterium. The sulfate reducing bacteria can effectively solve the problem of low sulfate tolerance concentration of the existing sulfate reducing bacteria.

The invention discloses a slow-release compound flocculant for reducing acidity of mine water. The slow-release compound flocculant is prepared from the following raw materials in percentage by weight: 87wt%-95wt% of an inorganic compound flocculant and 5wt%-13wt% of a soluble coating material, wherein the soluble coating material wraps the inorganic compound flocculant; the inorganic compound flocculant is a mixture of a microbial carbon source, a coagulant aid and an adsorbent; the soluble coating material is a culture medium mixed with a microbial agent; and the microbial agent is a mixed agent prepared from a desulfovibrio agent, a colourless sulfur bacteria agent and a gluconobacter agent. According to the slow-release compound flocculant, the characteristics of inorganic and biological flocculants are integrated; a chemical dosing mode is improved; the interaction efficiency of various flocculants is increased by multi-step dissolving and slow-release processes; and the efficiency of the agent is improved.

The invention discloses a composite bacterium, which can be used for the degradation of COD (Chemical Oxygen Demand) and / or organic sulfides and pretreatment for the degradation of garlic processing wastewater. The composite bacterium is obtained by mixing fermentation broth of bacillus megaterium, pseudomonas stutzeri, aspergillus oryzae, klebsiella oxytoca and desulfovibrio desulfuricans, of which bacterial cell concentrations are 20 to 30 percent, 10 to 15 percent, 15 to 35 percent, 10 to 35 percent and 5 to 15 percent respectively. The composite bacterium can be applied to the degradation of COD and / or organic sulfides, and can also be applied to the pretreatment for the degradation of the garlic processing wastewater. By such a biological pretreatment method for the degradation of the garlic processing wastewater, the influence of changes in the conditions of COD and organic sulfide concentrations, water volumes and the like on a degradation effect can be reduced, and use of a plurality of chemical reagents in a subsequent process can be reduced and even avoided; the pretreated wastewater can be discharged up to the standard in an activated sludge treatment system.

The invention discloses an intestinal tract regulating type feed additive based on ulva lactuca polysaccharide dissolved out under high pressure as well as a preparation method and an application of the intestinal tract regulating type feed additive. Water-soluble polysaccharide of the feed additive accounts for 80% or above of the total sugar content; monosaccharide composition measured by a pre-column derivatization chromatography consists of rhamnose (25.0%, DW), xylose (6.0%, DW), glucuronic acid (9.0%, DW) and iduronic acid (3.1%, DW), wherein the sulfate group content is greater than 15%. The feed additive provided by the invention has a regulating effect on the intestinal health of weaned piglets, and the result shows that the product can effectively reduce the diarrhea rate of thepiglets and improve the feed-weight ratio of the piglets to a certain extent; the feed additive can improve the intestinal flora structure of piglets, inhibit the proliferation of intestinal harmful bacteria such as Mollicutes _ RF9 (flexible membrane bacteria), Escherichia-Shigella (Escherichia-Shigella) and Desulfovibrio (Desulfurization Vibrio) and the like, and promote the proliferation of bifidobacteria and clostridium butyricum; intestinal health of piglets is promoted, and intestinal inflammation is relieved; fermentation in the intestinal tract can promote the production of lactic acid, acetic acid, propionic acid and total SCFA.

The invention relates to a novel microorganism flora mixture and a mixed nutrient medium thereof. The nutrient medium is filter liquor which is prepared by taking plant powder, mineral composition, animal powder, organic acid and the like as raw materials, adding sewage or comprehensive sewage in cities to be processed, mixing, fermenting and filtering. The microorganism flora mixture is a microorganism preparation which is prepared by the following steps: respectively selecting fusarium oxysporum, aspergillus fumigatus, sphaerotilus, vorticella microstoma, photosynthetic bacteria, methanebacteria, desulphovibrio, nitrobacterium, pseudomonas fluorescens and other bacterial strains from obligate aerobes group, anaerobic bacteria group and facultative aerobe group preferentially, then expanding and cultivating, inoculating into the above mixed nutrient medium, mixing, cultivating and domesticating for 15-30 days at the temperature of 20-45 DEG C. The mixed nutrient medium in the invention can cultivate, propagate and domesticate flora mixture with the combination advantages of flora; the flora has stable integrity, no easy variation and strong adaptability, and the invention breaks through the technical bottlenecks of easy degeneracy of microorganism flora, easy decrease of biological activity and slow proliferation and expansion.

Biomarkers for diabetes and usages thereof are provided. And the biomarkers are Akkermansia muciniphilacBacteroides intestinaliscBacteroides sp. 20_3cClostridium bolteaec Clostridium hathewayicClostridium ramosumcClostridium sp. HGF2cClostridium symbiosumc5 Desulfovibrio sp. 3_1_syn3cEggerthella lentacEscherichia colicClostridiales sp. SS3 / 4c Eubacterium rectalecFaecalibacterium prausnitziicHaemophilus parainfluenzaecRoseburia intestinalis and Roseburia inulinivorans.