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Method for preparing strain and microbial agent for heavy metal contaminated water treatment

A biological bacterial agent and heavy metal technology, applied in the biological field, can solve problems such as heavy metal water pollution, and achieve the effect of improving the pH of water

Inactive Publication Date: 2016-03-30
POWERCHINA ZHONGNAN ENG +1
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0004] The present invention aims at the existing problem of heavy metal water pollution, and provides a preparation method of bacteria and microbial agents for industrial and acid mine wastewater heavy metal water pollution treatment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Preparation of medium:

[0020] Strain activation medium is Starkey medium: K 2 HPO 4 0.5g, NH 4 Cl1.0g, Na 2 SO 4 1.0g, CaCl 2 2H 2 O0.1g, MgSO 4 ·7H 2 O2.0g, yeast extract 1.0g, 70% sodium lactate 5.0g, distilled water 1000mL. Add 1% (NH 4 ) 2 Fe(SO 4 ) 2 Solution, add 5mL per 100mL medium before use. After dissolving, use 1N NaOH to adjust the pH value to about 7.4, divide into several test tubes, cover with silica gel plugs, and steam sterilize at 121°C for 20 minutes for later use.

[0021] The seed medium is modified Postgate's medium: Na 2 SO 4 0.5g, NH 4 Cl1.0g, CaCl 2 0.1g, K 2 HPO 4 0.5g, Fe(SO 4 ) 2 ·7H 2 O0.002g, MgSO 4 2.0g, sodium lactate 5.0g, yeast extract 1.0g, ascorbic acid 0.1g, distilled water 1000mL. Another 0.6% cysteine ​​hydrochloride solution was added to the culture medium at a ratio of 10% before use. After dissolving, use 1N NaOH to adjust the pH value to about 7.4, then pour it into a conical flask, and steam sterili...

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Abstract

The invention discloses a method for preparing a strain and a microbial agent for heavy metal contaminated water treatment. The strain is a sulfate reducing bacterium Desulfovibrio oxamicusw, has a preservation number of CGMCC 9548, and is named D. oxamicusw CGMCC 9548. The D. oxamicusw CGMCC 9548 has a removal rate on SO4<2->, Pb, Cd, As and W to be over 80 percent, so that heavy metals in a water body can be removed, and the pH value of the water body can be increased. The strain is applied to heavy metal water contamination treatment of industrial and acid mine wastewater. The method provides a developed fermentation method to large-scale fermentation production of D. oxamicusw CGMCC 9548 strain microbial agent, and the product strain density is over 1012. The microbial agent can be used for removing heavy metals in a water body and increasing the pH value of the water body, and can be applied to heavy metal water contamination treatment of industrial and acid mine wastewater.

Description

technical field [0001] The invention belongs to the field of biological technology, and relates to a preparation method of a strain and a microbial agent for heavy metal polluted water treatment. Background technique [0002] Sulfate-reducing bacteria (SRB) is a general term for bacteria that can perform sulfate-reducing metabolic reactions. SRB is a class of strictly anaerobic bacteria with diverse morphology and nutrition, and uses sulfate as electron acceptor. Unlike organic matter, heavy metal ions cannot be biodegraded in the environment, so once they enter the environment, they will continue to accumulate in the environment, causing long-term environmental pollution. At present, the treatment methods for heavy metal wastewater mainly include precipitation method (formation of hydroxide, carbonate, sulfide, etc.), ion exchange method, electrodialysis and reverse osmosis, etc., and the cost of these treatment methods is relatively high. The SRB method for treating indu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/20
CPCC02F3/34C02F2101/20C12N1/205C12R2001/01
Inventor 郭沨杜刚李勇高云涛杜成琼张振宇陈湘斌
Owner POWERCHINA ZHONGNAN ENG
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