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Biosensor for detecting zearalenone and preparation method thereof, and method for detecting zearalenone by using same

A zearalenone and biosensor technology is applied in the field of biosensors for detecting zearalenone, and the field of detecting zearalenone can solve the problems of long time consumption, complicated operation steps and high detection cost, and achieves the Low cost, good specificity, high sensitivity effect

Inactive Publication Date: 2019-09-20
QINGDAO AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the cumbersome scheme of the ZEN detection method in the prior art, complicated operation steps, high detection cost, and long time spent, the present invention provides a fluorescent aptasensor using graphene oxide as a fluorescent quencher, and Method for detecting zearalenone using the sensor

Method used

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  • Biosensor for detecting zearalenone and preparation method thereof, and method for detecting zearalenone by using same
  • Biosensor for detecting zearalenone and preparation method thereof, and method for detecting zearalenone by using same
  • Biosensor for detecting zearalenone and preparation method thereof, and method for detecting zearalenone by using same

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Experimental program
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Embodiment

[0034] (1) ZEN aptamer self-assembles with graphene oxide to form a detection platform

[0035] Pretreatment: ZEN aptamer was treated with PBS buffer (NaCl 136.89mM, KCl 2.67mM, NaCl 2 HPO 4 8.1mM,KH 2 PO 4 1.76Mm, pH=7.4) dissolved to a concentration of 1 μM, placed at 4°C and protected from light with tinfoil. The concentration of ZEN aptamer working solution is 1 μM. The purchased 500 μg / mL graphene oxide suspension was diluted with ultrapure water to a concentration of 250 μg / mL, ultrasonicated at 200 W for 1 h, and placed at 4 °C for use.

[0036] Add 20 μL of ZEN aptamer (1 μM) to 20 μL of GO with a concentration of 20 μg / mL, shake well and incubate at room temperature for 10 min to fully combine ZEN aptamer with graphene oxide, and the ZEN aptamer The fluorescence is completely quenched by graphene oxide, forming a ZEN aptamer-graphene oxide complex.

[0037] The ZEN aptamer is:

[0038] 5'-Cy3-CTACCAGCTTTGAGGCTCGATCCAGCTTATTCAATTATACCAGCTTATTCAATTATACCAGC-3'. ...

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Abstract

The invention discloses a biosensor for detecting zearalenone and preparation method thereof, and a method for detecting zearalenone by using the same, and belongs to the technical field of harmful substance detection. The biosensor for detecting zearalenone is formed by self-assembling a ZEN aptamer which is a nucleic acid aptamer capable of specifically recognizing zearalenone and labeled by a fluorescent group at the 5' end and graphene oxide. The operation steps of the biosensor for detecting zearalenone are simple and clear, the cost is low, the specificity is good, the detection range is 1-500 ng / mL, and the sensitivity is high.

Description

technical field [0001] The invention belongs to the technical field of harmful substance detection, and in particular relates to a biosensor for detecting zearalenone, a preparation method thereof and a method for using the sensor to detect zearalenone. Background technique [0002] Zearalenone is a mycotoxin produced by a variety of Fusarium species with estrogen-like effects. Zearalenone is widely distributed in grains, causing huge economic losses to crops. Zearalenone has strong reproductive toxicity and teratogenic effect, seriously affects the reproductive development of animals, and brings great economic losses to animal husbandry. Zearalenone will eventually enter the human body along the food chain, causing great harm to human reproductive development, cell proliferation and organ function. Contamination of feed and food by zearalenone not only seriously affects the agricultural economy, but also brings serious food safety problems and threatens human health. The...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6402G01N21/6486
Inventor 杨庆利王琦吴薇颜春蕾都晗赵海燕于春娣朱英莲
Owner QINGDAO AGRI UNIV
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