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Ultra-sensitive detection method of ochratoxin A based on gold core-silver satellite chiral assembly body

A technology of ochratoxin and assembly, applied in the field of analytical chemistry, can solve the problems of high consumption, monoclonal antibody preparation and screening, etc., and achieve the effect of high sensitivity and low detection limit

Active Publication Date: 2014-12-24
JIANGNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

ELISA has high requirements for monoclonal antibodies, and the preparation and screening of monoclonal antibodies is a heavy and costly task

Method used

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  • Ultra-sensitive detection method of ochratoxin A based on gold core-silver satellite chiral assembly body
  • Ultra-sensitive detection method of ochratoxin A based on gold core-silver satellite chiral assembly body
  • Ultra-sensitive detection method of ochratoxin A based on gold core-silver satellite chiral assembly body

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Embodiment 1

[0030] (1) Construction of detection sensors

[0031] (1) Synthesis of gold nanoparticles with a diameter of 35 nm

[0032] Gold nanoparticles with a diameter of 35 nm are synthesized by the seed growth method.

[0033] 13 nm gold seeds are synthesized by reducing chloroauric acid with trisodium citrate: the conical flask used in the reaction is soaked in freshly prepared aqua regia for 24 hours in advance, rinsed with ultrapure water to clean, and dried at 37°C for later use. Add 2 mL of 10 mM chloroauric acid and 38 mL of ultrapure water into a clean Erlenmeyer flask, heat to boiling, add 2 mL of 38.8 mM trisodium citrate, and boil for 20 minutes to obtain gold seeds with a diameter of 13 nm.

[0034] Synthesis of gold nanoparticles with a diameter of 35nm: Add 2mL of 10mM chloroauric acid, 0.1mL of 10mM silver nitrate, 42.5mL of ultrapure water, 2mL of gold seeds with a diameter of 13nm, and add 7.5mL under stirring. 5.3 mM ascorbic acid solution was pumped in at a speed of 30 mL / ...

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Abstract

The invention discloses an ultra-sensitive detection method of ochratoxin A based on a gold core-silver satellite chiral assembly body, and belongs to the field of analytical chemistry. The method mainly comprises the following steps: (1) synthesizing gold nanoparticles with the particle size of 35 nm; (2) synthesizing silver nanoparticles with the particle size of 8 nm; (3) modifying aptamers on the gold nanoparticles; (4) modifying part of complementary sequences of aptamers on the silver nanoparticles; (5) assembling a gold core silver satellite structure; (6) performing circular dichroism spectrum detection, and creating a standard curve of gold and silver chiral signal superposition and ochratoxin A concentration. The invention provides a method for detecting ochratoxin A by a chiral signal of the gold core silver satellite structure. Compared with a conventional detection method, the method provided by the invention is low in cost, high in sensitivity, convenient and quick, and has a very good practical application prospect.

Description

Technical field [0001] The invention relates to an ultra-sensitive detection method of ochratoxin A based on the chiral structure assembly of a gold core-silver satellite, and belongs to the field of analytical chemistry. Background technique [0002] Ochratoxin A is produced by strains of Penicillium verrucosum, Penicillium violaceum, and Penicillium arcuate, and is most commonly found in moldy grains and feed. After animals ingest moldy feed, meat products may contain this toxin. Ochratoxin A has a strong toxic effect on animal liver and kidney, and can cause kidney damage. A large amount of toxins may also cause inflammation and necrosis of the intestinal mucosa of animals. It has been observed in animal experiments that it also has teratogenic effects. [0003] The traditional detection methods of ochratoxin A (OTA) are mainly based on instruments, such as liquid chromatography-mass spectrometry, high performance liquid chromatography, etc. The detection cost is very high, a...

Claims

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Application Information

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IPC IPC(8): G01N21/19
Inventor 徐丽广赵雪利胥传来匡华马伟刘丽强宋珊珊吴晓玲
Owner JIANGNAN UNIV
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