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Ratio electrochemical detection method of ochratoxin A

An ochratoxin and detection method technology, applied in the field of biosensing, can solve the problems of electrochemical sensors being easily interfered by external factors, low reliability accuracy, insufficient accuracy, etc., and achieves high reliability, improved reliability and high reliability. Accuracy and high sensitivity

Active Publication Date: 2019-12-27
JIANGSU UNIV
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AI Technical Summary

Benefits of technology

This patented technology allows us to create an electrical system that can accurately measure small amounts (microgram) or very low concentrations of chemical substances such as glucose by measuring changes caused when certain molecules react chemically at specific locations on their surface. It also includes techniques to enhance the efficiency of this process through enhancing the interaction between these reagents and target biomolecules. Overall, it provides technical benefits over existing methods used alone.

Problems solved by technology

The technical problem addressed in this patents relates to developing a simpler, faster, less expensive method that could detect very small amounts of oxytoxic compounds called ochratoxicosis or cerebralotorism glycerolum-3 fatty acid esterase (CagAT).

Method used

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  • Ratio electrochemical detection method of ochratoxin A
  • Ratio electrochemical detection method of ochratoxin A
  • Ratio electrochemical detection method of ochratoxin A

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Embodiment 1

[0034] A ratiometric electrochemical detection method for ochratoxin A, comprising the following steps:

[0035] (1) Grind a gold electrode (AuE) with a diameter of 3mm with 0.3μm and 0.05μm Al2O3 powder in turn, and ultrasonically clean the surface residue in ethanol and water in turn, and then in 0.1M sulfuric acid solution at 100mV / s The scanning speed, the potential of -0.2-1.6V use cyclic voltammetry to electrochemically clean the electrode;

[0036] (2) Modify 6 μL of ferrocene-labeled complementary DNA (Fc-cDNA) with a concentration of 1 μM onto the electrode treated in step (1), place it at room temperature for 8 hours, and use Au-S bonds to fix Fc-cDNA on the gold electrode surface;

[0037](3) 6 μL of mercaptohexanol (MCH) with a concentration of 1 mM was modified on the electrode treated in step (2), and incubated at room temperature for 1 hour to block the non-specific binding site of gold;

[0038] (4) Modify 6 μL of ochratoxin A aptamer (Aptamer) with a concent...

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Abstract

The present invention belongs to the field of biosensing technologies, and relates to a method for preparing a ratio electrochemical aptamer sensor for detecting ochratoxin A, and the method is used for detecting the ochratoxin A (OTA). The ratio sensing policy is constructed by sequentially modifying ferrocene marked complementary DNA (Fc-cDNA), ochratoxin A aptamer (Aptamer) and auxiliary complementary DNA (hDNA) on a gold electrode, double current signals IFc and IMB (which are respectively oxidized electric currents of Fc and MB) are output by using a target-induced comformational change,and OTA is detected by using a ratio signal (IFc/IMB) quantization sum of IFc and IMB. The obtained ratio electrochemical aptamer sensor can implement highly sensitive and highly reliable detection onthe OTA, a detection linearity range is 10 pg/mL to 10 ng/mL, and a detection limit is 3.3 pg/mL. The present invention aims to develop a ratio electrochemical aptamer sensor that is high in sensitivity, good in selectivity, and high in reliability, and provide a reliable sensing platform for measuring OTA in an actual sample.

Description

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Claims

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Application Information

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Owner JIANGSU UNIV
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