Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Construction method and application of SERS aptasensor based on nano-assembly structure

An aptamer sensor and nano-assembly technology, which is applied in the direction of instruments, measuring devices, scientific instruments, etc., can solve the problems of measurement substance interference, inability to guarantee the specificity and selectivity required for detection, complex food matrix components, etc., and achieve simple operation , the effect of improving the sensitivity

Active Publication Date: 2017-10-24
ZHEJIANG UNIV
View PDF6 Cites 19 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the composition of the food matrix is ​​complex, and it is easy to interfere with the measured substances. Relying solely on SERS technology cannot guarantee the specificity and selectivity required for detection. Generally, it is necessary to use chemometric methods or establish models for discrimination and identification, especially for OTA. Molecules, the combination of SERS technology and nucleic acid aptamers can just make up for this deficiency

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Construction method and application of SERS aptasensor based on nano-assembly structure
  • Construction method and application of SERS aptasensor based on nano-assembly structure

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0046] The present invention will be further described below with reference to the drawings and embodiments.

[0047] The embodiment of the present invention is as follows:

[0048] 1) Preparation of gold nanorod DNA probes (AuNRs-DNA)

[0049] 1.1) The first DNA modified by carboxyl group (sequence is 5'-AGTCACAGTGAGACTTTTTTT-CH(6)-SH-3', the base sequence is as SEQ ID No. 1) is mixed with freshly prepared TCEP solution and kept at room temperature Let stand for 1 hour, the molar ratio of TCEP to DNA is TCEP:DNA=10:1;

[0050] 1.2) Adjust the pH of 1×TBE buffer (containing 0.02% SDS and 500mM NaCl) to 3.0 with 1M HCl solution, and then freshly prepare and purified gold nanorods (with an average length of 65.8nm, The average width is 23.5nm, the radial ratio is 2.8) and the activated DNA solution obtained in step 1.1) is added to the pH-adjusted TBE buffer, mixed and shaken for 10 minutes. The molar ratio of DNA to gold nanorods is DNA: AuNRs=1000:1, then centrifuged and resuspended...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a construction method and application of SERS aptasensor based on nano-assembly structure. The method comprises the following steps: respectively modifying surfaces of gold nano-rod and gold nano-particle with two kinds of DNA complementary with an ochratoxin A aptamer segment part to form two nano-probes; then assembling an assembly nano-structure under complementary pairing connection of the two nano-probes and the DNA aptamer, and labeling a Raman beacon molecule on the assembly nano-structure; and adding a solution of a substance for detecting, and detecting the concentration of the assembly nano-structure by utilizing the fact that the SERS signal intensity of the assembly nano-structure is influenced by the substance for detecting. By adopting the method, reaction in a liquid environment is uniform, the detection has high sensitivity and high specificity, only one-step reaction is required, and the operation is simple.

Description

Technical field [0001] The invention relates to a construction method and application of a SERS aptamer sensor based on a nano-assembled structure, in particular to the application in the detection of ochratoxin A. Background technique [0002] Ochratoxin A (OTA) can cause serious harm to human health through contaminated agricultural products and food, and can cause cancer and even death. Worldwide, the EU has the most stringent requirements for mycotoxins limits. The limits of OTA in agricultural products and foods generally range from 0.3-50μg / kg (including infant food). Considering that the sample extraction process is generally diluted, which requires that the new detection method developed must have extremely high sensitivity. At the same time, the food matrix components are complex and easily interfere with the measured substances, so the selectivity or specificity of the method is particularly important. In addition, the detection method must be simple and practical to ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/532G01N21/65
CPCG01N21/658G01N33/532
Inventor 徐霞徐程楠应义斌
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com