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Electrochemical sidestream immune quantitative test paper sensor based on microgap array electrode and method thereof for detecting biotoxin

An immunoquantitative and electrochemical technology, applied in the field of electrochemical lateral flow immunoquantitative test paper sensor to detect ochratoxin A or fumonisin B1, it can solve unsuitable rapid on-site screening, complex extraction and purification steps, and detection of samples Limited quantity and other issues, to achieve the effect of authentic and reliable data, low cost, and portable instruments

Inactive Publication Date: 2009-08-19
湖南省宜生科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no established method for the detection of fumonisin B in China 1 Among the many detection methods of fumonisin B1, thin layer chromatography (TLC), gas chromatography (GC), capillary electrophoresis, high performance liquid chromatography (HPLC), liquid / mass chromatography (LC / MS), liquid secondary ion probe mass spectrometry (liquid-S IMS) and other detection methods, these detection methods have high sensitivity and specificity, but all require complex extraction and purification steps, and the number of detection samples per unit time is limited. , and requires more expensive instruments and equipment, which takes a long time and is not suitable for rapid on-site screening, and most of the grassroots laboratories in my country do not have these conditions at all, which limits the popularization and application of these methods

Method used

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  • Electrochemical sidestream immune quantitative test paper sensor based on microgap array electrode and method thereof for detecting biotoxin
  • Electrochemical sidestream immune quantitative test paper sensor based on microgap array electrode and method thereof for detecting biotoxin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Utilize this sensor to the detection of the recovery rate of fumonisin B1 in corn: take the 120 mesh screened corn (undetected fumonisin B1) that has been measured and crushed, put it in a 250mL Erlenmeyer flask with a stopper, Add 10.0ng / mL fumonisin B1 and mix well. Shake with 100mL of methanol:water (3:1, V / V) mixed solution for 20min, stand for extraction for 10min, dilute with 20mL of water, and cover tightly to prevent leakage. Centrifuge the mixed solution (2500-3000r / min, 10-15min), take 100 μL of the supernatant and drop it on the sample liquid absorption pad on the surface of the prepared electrochemical lateral flow immunoassay paper for fumonisin B1. When the sample liquid reaches the detection reaction area, it is left to stand at room temperature for 8-12 minutes, and then the conductance signal related to the concentration of fumonisin B1 antigen is obtained from the electrode. Record the change value. The concentration of fumonisin B1 was calculated ac...

Embodiment 2

[0028] Use this sensor to detect ochratoxin A in moldy peanut samples: Take 250.0g of moldy peanut samples that have been crushed through a 120-mesh sieve, put them in a 250mL conical flask with a stopper, add 200mL of 0.2mol / L phosphate buffer solution , homogenized into a homogenate, and mixed. Take 50 mL of supernatant by filtration, shake with 100 mL of methanol:water (3:1, V / V) solution for 20 min, let stand for extraction for 10 min, and cover tightly to prevent leakage. Centrifuge the mixed solution (2500-3000r / min, 10-15min), take 100 μL of the supernatant and drop it on the sample liquid absorption pad on the surface of the prepared electrochemical lateral flow immunoassay paper for ochratoxin A. When the sample liquid reaches the detection reaction area, it is allowed to stand at room temperature for 8-12 minutes, and then the conductance signal related to the concentration of ochratoxin A antigen is obtained from the electrode. Record the change value. The concent...

Embodiment 3

[0030] Use this sensor to detect fumonisin B1 in moldy corn samples: Take 200.0g of moldy corn samples that have been crushed through a 120-mesh sieve, put them in a 250mL conical flask with a stopper, add 0.2mol / L phosphate buffer solution 200mL, homogenize into a homogenate, and mix well. Take 50 mL of the supernatant by filtration, shake it with 100 mL of methanol:water (3:1, V / V) solution for 20 min, let it stand for extraction for 10 min, and dilute with 20 mL of water. Centrifuge the mixed solution (2500-3000r / min, 10-15min), take 100 μL of the supernatant and drop it on the sample liquid absorption pad on the surface of the prepared electrochemical lateral flow immunoassay paper for fumonisin B1. When the sample liquid reaches the detection reaction area, it is left to stand at room temperature for 8-12 minutes, and then the conductance signal related to the concentration of fumonisin B1 antigen is obtained from the electrode. Record the change value. The average valu...

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Abstract

The invention relates to an electrochemical lateral-flow immunity quantitative test paper sensor based on microclearance array electrode, and a method thereof used for detecting biological toxins such as ochratoxin A and fumonisin B1. The sensor includes two parts, namely, immune chromatographic analysis test paper strip and electrochemical detecting part. The fast detecting test paper sensor has strong specificity, can realize quantitative detection, can be used at the temperature between 4 and 40 DEG C, and the result can be observed after ten minutes, thus being suitable for units or individuals to quickly detect ochratoxin A and fumonisin B1 in animal derived food samples, and being expected to become an effective technical means for the field screening of ochratoxin A and fumonisin B1 in food and feed samples.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an electrochemical lateral flow immunoquantitative test paper sensor based on a micro-gap array electrode and a method for detecting ochratoxin A or fumonisin B1 using the sensor. Background technique [0002] Ochratoxins are secondary metabolites produced by certain species of Aspergillus and Penicillium. It is very likely to enter the human body through the food chain, thus posing a potential threat to human health. Among them, Ochratoxin A (OTA) is the most toxic, toxic to the immune system, and has teratogenic, carcinogenic and mutagenic effects. OTA pollution levels in different agricultural products vary greatly, about 1.4% and 0.6% of agricultural products have OTA pollution levels exceeding 5 and 20 μg / kg, of which 1.2% and 0.3% of samples are OTA in grain and its products The OTA contamination level of samples with content exceeding 5μg / kg, 0.3% and 0.05% was h...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N30/02G01N27/327G01N33/569
Inventor 彭新凯蒋健晖胡朝晖陈利国沈国励
Owner 湖南省宜生科技有限公司
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