212 results about "Ochratoxins" patented technology

Methods are provided for isolating a nutrient from coffee cherries or for producing a food product that comprises a coffee cherry or portion thereof. It is particularly preferred that coffee cherries will have an extremely low concentration of mycotoxins, including various aflatoxins, fumonisins, ochratoxins, and / or vomitoxin (DON, deoxynivalenol).

The invention discloses a method for portably and rapidly detecting ochratoxin A. The method comprises the following steps: first, preparing sucrose invertase-terminal alkyne modified DNA; subsequently, combining biotin-nitrine modified DNA with the surface of a magnetic bead, adding the ochratoxin A with different concentration, and mixing; adding the previously-prepared sucrose invertase-terminal alkyne modified DNA, a CuSO4 solution and an ascorbic acid solution, wherein the ascorbic acid solution can be used for reducing Cu(II) to obtain a Cu(I) compound so as to catalyze the biotin-nitrine modified DNA and the sucrose invertase-terminal alkyne modified DNA to have a click chemistry reaction; taking a supernatant liquor after the reaction is ended, then adding the supernatant liquor into a sucrose solution, wherein the sucrose invertase can effectively catalyze the sucrose in the solution to be transformed into glucose; and finally, measuring by adopting a glucometer. The method is used for organically combining the rapid reaction advantage of the click chemistry reaction with the high specificity characteristic of an aptamer, and adopting the simple and portable glucometer to acquire data, thus the operation complexity and detection cost are greatly reduced, and the sensitivity and selectivity are improved.