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Preparation method of ratio electrochemical aptamer sensor for simultaneously detecting aflatoxin B1 and ochratoxin A

An aptamer sensor, ochratoxin technology, applied in the field of electrochemical sensing, achieves high precision, high sensitivity, and improved precision

Active Publication Date: 2020-05-19
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Currently, there is no research report on a ratiometric electrochemical aptasensor for the simultaneous detection of AFB1 and OTA

Method used

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  • Preparation method of ratio electrochemical aptamer sensor for simultaneously detecting aflatoxin B1 and ochratoxin A
  • Preparation method of ratio electrochemical aptamer sensor for simultaneously detecting aflatoxin B1 and ochratoxin A
  • Preparation method of ratio electrochemical aptamer sensor for simultaneously detecting aflatoxin B1 and ochratoxin A

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Embodiment 1

[0033]A method for preparing a ratiometric electrochemical aptasensor for simultaneous detection of aflatoxin B1 and ochratoxin A, the construction and detection process of the ratiometric electrochemical aptamer sensor is shown in the attached figure 1 shown;

[0034] Specifically include the following steps:

[0035] (1) Grind a gold electrode (AuE) with a diameter of 3mm with 0.3μm and 0.05μm Al2O3 powder in turn, and ultrasonically clean the surface residue in ethanol and water in turn, and then in 0.1M sulfuric acid solution at 100mV / s Electrochemical cleaning of the electrode using cyclic voltammetry at a scan rate of -0.2 to 1.6V;

[0036] (2) Modify 6 μL of AQ-hDNA with a concentration of 2 μM on the electrode treated in step (1), place it at room temperature for 8 hours, and use gold-sulfur bonds to fix AQ-hDNA on the surface of the gold electrode;

[0037] (3) 6 μL of mercaptohexanol (MCH) with a concentration of 1 mM was modified on the electrode treated in step (...

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Abstract

The invention belongs to the technical field of electrochemical sensing, and relates to a preparation method of a ratio electrochemical aptamer sensor for simultaneously detecting AFB1 and OTA. The sensor is prepared by assembling AQ-hDNA, Fc-Apt1 and MB-Apt2 on a gold electrode in sequence, the combination of a target object and an aptamer causes Fc-Apt1 and MB-Apt2 to be stripped from a surfaceof the electrode such that ratio signals IFc / IAQ and IMB / IAQ are reduced, and the purposes of respectively quantifying and detecting AFB1 and OTA are achieved; the linear detection range of the sensoron AFB1 is 10 pg / mL-3 ng / mL, and the detection limit is 3.3 pg / mL; the detection linear range on OTA is 30 pg / mL-10 ng / mL, and the detection limit is 10.0 pg / mL; two substances are detected at the same time, and the method has the characteristics of high sensitivity, good selectivity, high accuracy and the like.

Description

technical field [0001] The invention belongs to the technical field of electrochemical sensing, and relates to a preparation method of a ratiometric electrochemical aptamer sensor for simultaneously detecting aflatoxin B1 and ochratoxin A. Background technique [0002] Aflatoxin B1 (AFB1) and ochratoxin A (OTA) are teratogenic, nephrotoxic and carcinogenic mycotoxins produced by specific fungi in agricultural products under high temperature and high humidity conditions. AFB1 and OTA can enter the food chain through contaminated food and animal feed, causing serious harm to human health. The International Agency for Research on Cancer (IARC) has classified AFB1 as a category 1 carcinogen (strongly carcinogenic to humans), while OTA is classified as a category 2B carcinogen (potentially carcinogenic to humans). Due to the simultaneous existence of multiple fungi, AFB1 and OTA often exist in the same agricultural product, such as moldy wheat or corn. The coexistence of mycoto...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/38G01N27/48G01N33/53
CPCG01N27/3276G01N27/3277G01N27/38G01N27/48G01N33/5308
Inventor 由天艳朱成喜刘东李玉叶申秀丽
Owner JIANGSU UNIV
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