77 results about "Field screening" patented technology

The invention relates to an electrochemical lateral-flow immunity quantitative test paper sensor based on microclearance array electrode, and a method thereof used for detecting biological toxins such as ochratoxin A and fumonisin B1. The sensor includes two parts, namely, immune chromatographic analysis test paper strip and electrochemical detecting part. The fast detecting test paper sensor has strong specificity, can realize quantitative detection, can be used at the temperature between 4 and 40 DEG C, and the result can be observed after ten minutes, thus being suitable for units or individuals to quickly detect ochratoxin A and fumonisin B1 in animal derived food samples, and being expected to become an effective technical means for the field screening of ochratoxin A and fumonisin B1 in food and feed samples.

The invention discloses an earth-rock dam construction material grain composition examination method based on digital image processing, and relates to the field of dam construction material grain composition. The method comprises the following steps that S1. digital images of grains are acquired; S2. processing, recognition and information extraction and conversion of the digital images are performed and then a composition curve based on image recognition is constructed; S3. a mathematical model is constructed through nonlinear fitting of the composition curve; and S4. a training database of the one-to-one correspondence relationship of the determined image recognition mathematical model parameters and field screening mathematical model parameters is constructed. The beneficial effects of the method are that the learning and training database of the field screening test composition curve data and the image recognition composition curve data of large samples is established to form contrast, then the function relationship between the two is determined based on an intelligent optimization algorithm and error is corrected by using the function relationship so that a set of rapid and accurate dam construction material composition quality examination method is formed.

The invention provides a method for preparing fast detecting test paper of illegal cooling oil. The method includes the following steps of sequentially preparing immune colored latex microspheres, an immune colored latex reaction pad and a nitrocellulose (NC) membrane and assembling a colored latex test strip. By means of the detecting test paper of the illegal cooking oil, detection sensitivity is high, a plurality of indexes can be simultaneously detected, a comprehensive and systematic detection method is provided for detecting the illegal cooking oil, and detection costs are relatively reduced; the quality of the detecting test paper can be guaranteed for two years at normal temperatures, the quality guarantee period is effectively prolonged, and preservation conditions are reduced; non-professional persons can use the detecting test paper to accomplish whole detection, so that the operation is simple, and popularization of the method is facilitated; and the whole detection process can be accomplished in 10 minutes at the soonest, and the detecting test paper is suitable for fast field screening.

The invention discloses an RPA technology-based marburg virus detection kit and an application thereof. An experiment proves that a target gene can be effectively amplified by an RPA primer and a probe of a marburg virus, the specificity reaches 100% and sensitivity is 1*10<2>copies / reaction; and the virus can reach the level equivalent to the sensitivity of fluorescent quantitative PCR, and has no cross reaction with a zaire ebola pseudovirus, a dengue virus, a hemorrhagic fever virus with renal syndrome and a Xinjiang hemorrhagic fever virus nucleic acid. The RPA isothermal amplification system is fast in reaction and wide in temperature range, effective amplification of a target gene can be achieved under the condition of 37-42 DEG C, the method can be used for fast field detection of an infectious nucleic acid of the marburg virus, and an available fast detection method is provided for field screening of a pathogen; and meanwhile, the marburg virus detection kit also has the important significance in prevention of infection transmission of the marburg virus in China and inspection and quarantine in affected areas and entry and exit ports.

The invention discloses a novel coronavirus kit and a method for detecting nucleic acid of the novel coronavirus by a single closed-tube one-step method. The kit for detecting the nucleic acid of thenovel coronavirus by the single closed-tube one-step method comprises a primer, a padlock probe and a PCA standard substance, wherein the PCA standard substance has a nucleotide sequence shown as SEQID No.1; the padlock probe has a nucleotide sequence shown as SEQ ID No.2; and the primer has a nucleotide sequence shown as SEQ ID No.3. According to a field rapid detection product for nucleic acidof 2019-CoV, the detection time is shortened; the convenience degree is improved; the sooner and focused diagnosis is promoted; and rapid diagnosis of suspected patients and field screening of close contacts are realized.

The invention relates to a process of applying immune chromatography test paper to detect the target sequence of ribonucleotide. A detecting probe comprises identification sites of nicked endonuclease, the 3' portion of the probe is marked with biotin, and the 5'portion of the probe is marked with SMD (sulfametoxydiazine). The probe forms an integral reaction probe through incubating with magnetic beads which are marked with streptavidin. The probe is nicked into two sections by enzyme sites on the nicked endonuclease identification probe after the hybridization of the integral probe and the target sequence of ribonucleotide, which reduces the stability of the combination between the integral probe and the target sequence and separates with target sequence. The target sequence can be hybridized with another integral probe and be nicked by nick endonuclease. Therefore, a magnification mode of detection signals is formed in the reaction system. The section of the probe with magnetic beads is absorbed to deposit, and the section of the probe with SMD presents on a free state in solution after reaction solution being acted with magnetic field. The probe with SMD can be detected in detecting solution through using the immune chromatography test paper so as to reach the effect of detecting the target sequence. The process is simple, fast and economical, which is suitable to detect the target sequence of ribonucleotide in large-scale field screening.

The invention relates to a multiple detection method for field screening of toxicogenic Pseudomonas aeruginosa and belongs to the field of molecular biology. The method includes LAMP (loop-mediated isothermal amplification) primer design, establishment of a multiple LAMP reaction system, and detection of multiple LAMP products by multiple colloidal gold test strips. In the LAMP primer design, the software Primer Explorer V4 is used to design four LAMP primers for each of 6 sites of each of endogenous reference gene ecfX, toxin gene ExoU and toxin gene ExoS of Pseudomonas aeruginosa; the four LAMP primers include two outer primers F3 and B3 and two inner primers FIP and BIP. The multiple LAMP detection technique used herein combines with the means of multiple colloidal gold test strip detection, allowing the endogenous reference gene and toxin genes of Pseudomonas aeruginosa to be detected fast; the method has the advantages of high speed, good sensitivity, high efficiency, low cost, and the like.

The invention relates to the field of online auxiliary evaluation, in particular to a cognitive and brain image data integration evaluation method for Alzheimer's disease. According to the method, firstly, multi-field screening and evaluation of the cognitive function are carried out, the purposes of rapid screening and detailed evaluation of the cognitive function are achieved, then multi-mode magnetic resonance imaging data analysis is carried out, and the multi-mode magnetic resonance imaging data analysis comprises the steps of extracting hippocampus volume and shape analysis, calculating a function connection value of a cognitive-related brain function network, and calculating the cognitive-related brain function network. As an index in the aspect of neuroimaging, the brain function is evaluated. Carrying out cognitive and neural Bayesian joint modeling; and finally, classifying and identifying the specific change mode of the Alzheimer's disease by using supervised learning.

The invention belongs to the technical field of screening of agricultural germplasm resources, and particularly relates to a screening method for a selenium-enriched rice variety. According to the method, preliminary screening is performed at first through indoor cultivation, so that the blindness of the subsequent field screening test is reduced; South Henan black glutinous rice is used as a contrast material, so that the screening process is simple and feasible. In the indoor screening process, a selenium-enriched culture medium is used to provide a uniform and stable selenium source for rice seedlings; the accuracy of variety screening can be substantially improved; seeds are started to be cultivated in the initial stage of germination and growth; nutrition is mainly supplied by the seeds to the seedlings; the culture medium is relatively easy to prepare, and the screening method is easy to apply.

The present invention discloses a field screening method for a density-tolerant high-yield maize strain suitable for mechanical kernel reaping. The field screening method comprises the following steps: (1) preliminary selection of a maize strain with high seed vigor and high seedling drought resistance, i.e., selecting proper land plots for field plot trials, and screening out the maize strain with high seed vigor and good seedling drought resistance by calculating field seedling emergence and variation coefficients; (2) re-selection of a dense planting-tolerant high-yield maize strain, i.e., carrying out high-density planting management to the maize strain screened out in the step (1), and screening out a dense planting-tolerant maize strain with stable high yield according to disease resistances in combination with yield levels; and (3) final selection of a maize strain suitable for mechanical kernel reaping, i.e., carrying out wide field dense planting and management to the maizestrain screened out in the step (2), and screening out the maize strain suitable for mechanical kernel reaping according to a kernel water content at harvest time and mechanical reaping weight indices. The field screening method to which the present invention relates is capable of screening out a density-tolerant high-yield maize strain suitable for mechanical kernel reaping that fits the gentle-slope mountainous environment of Yunnan.

The invention provides the use of an ion mobility spectrometer for detecting gibberellins residues in crops and a method for detecting the gibberellins residues in the crops. The uses of the ion mobility spectrometer are expanded, the thought of detecting gibberellins by using the ion mobility spectrometer is disclosed for the first time, and an implementation method for detecting the gibberellins by using the ion mobility spectrometer is provided. The gibberellins are quickly and conveniently detected, the operating method is simple, the detection sensitivity is high, and the detection result accuracy is high. The gibberellins can be detected in a laboratory as well as a supermarket and a farmers market, and law enforcement officials can implement field screening and detection conveniently.

The invention discloses a breeding method for rape. The breeding method comprises the steps as follows: selecting a rape variety Zhenyou No.5 which is planted in a large area in production, and has the characteristics of high yield, good disease resistance and lodging resistance, outstanding comprehensive characters and wide applicability, and performing purification and rejuvenation; collecting and screening an excellent variety (line) which has relatively far genetic relationship with the Zhenyou No.5, and has the characteristics of double-low quality, good yielding ability, outstanding stress resistance and high oil content and performing purification and rejuvenation; taking the Zhenyou No.5 purification and rejuvenation line as a female parent and taking the screened distant related purification and rejuvenation line as a male parent, and performing castration and hybridization manually; harvesting seeds and planting in the same year to perform yield identification, performing field screening to obtain hybrid seeds with good comprehensive characters, high yield and strong advantages, and performing indoor seed test and quality determination in a combination way on the selected target combination; adopting a chemical hybridizing agent to perform chemical hybridizing on the Zhenyou No.5, and planting the male parent to perform seed production, so as to product hybrid seeds; performing a multi-point test on the produced seed target combination through chemical hybridizing, and testing the advantages and the wide adaptability of the produced seed target combination; performing pre-production, demonstration and popularization.

The invention discloses a visualized detection method of residual fluoroquinolones in sea foods. The method comprises the following steps of: preparing a specific antibody by coupling bovine serum albumin (used as a carrier protein) and fluoroquinolones with immunological technique; conjugating the specific antibody and red colloidal gold particles to obtain a colloidal gold-labeled antibody; coating and blocking a nitrocellulose membrane (used as a solid carrier) with another antibody; sequentially adding a sample solution, the colloidal gold-labeled antibody and a washing solution, and preparing a negative control from an extractive solution containing no fluoroquinolones; and compared with the negative control, and determining whether the sample is positive (containing fluoroquinolones) based on the existence of macroscopic red spots. The invention can be used for rapid field screening and detection of multiple residual fluoroquinolones; and has the advtanges of high sensitivity and accuracy, simple and rapid operation, no need of extra analytical apparatus, and macroscopic result.

The invention belongs to the technical field of oil-gas exploration and development, and particularly relates to a quantitative analysis method for pore volume increments of hydrated shale in different scales. According to the quantitative analysis method for the pore volume increments of the hydrated shale in different scales, saturation of a standard rock sample is carried out by adopting a first solvent which does not generate hydration reaction with the shale, data of original effective pore volume is obtained through testing, and then saturation testing is carried out by adopting water; and therefore, real change data of pores of different scales after shale hydration can be obtained. The method can quantitatively evaluate the absolute increase amplitude of the effective pore volumesof different scales in the shale under the hydration effect, and has practical significance for guiding field screening of shale reservoirs suitable for production increase of after-fracturing "soaking".

The invention discloses an induction method for embryogenesis and plant regeneration of in vitro haploid of macleaya cordata anther, which belongs to the field of plant tissue culture techniques. The method comprises the following steps: (1) preparation of a medium; (2) somatic embryogenesis; (3) germination and seedling formation of a somatic embryo; and (4) domestication, transplantation and the like of a tissue culture seedling. According to the invention, the induction method is a tissue culture method established with the macleaya cordata anther as an explant and can realize rapid, high-efficiency and large-scale production of high-quality macleaya cordata haploid plants; after field screening, the macleaya cordata haploid plants can be directly used as breeding materials for research on breeding and genomics.

The invention relates to a dielectric field screening concentrating machine and a metallurgical slag recovering method. The dielectric field screening concentrating machine comprises a supporting frame, a sorting box, an ore feeding part, a dielectric sieve screen, multiple electrode plates, a quick-return mechanism, a tailing outlet and a concentrate outlet. The tail portion of the dielectric sieve screen is provided with a rotary scraping plate connected to the concentrate outlet. The tailing outlet is located in the lower portion of the sorting box. The multiple electrode plates are installed on the dielectric sieve screen and the ore feeding part in parallel. An insulating layer is attached to the outside of each electrode plate. According to the machine, the heat-preserving no-heating method is adopted for enabling ore to be fully crystallized and grow up, additional energy is not needed, according to the crystallization differentiation principle, the efficiency ratio of crystallization and growth of enrichment phases of metallic oxide is maximized, the dielectric chaining and screening method is adopted for separating ultrafine ore, the process is the physical process, then the dielectric liquid physical method is adopted for carrying out recovering to ensure zero emission of pollutants in the whole process, and useful metallic oxide in the metallurgical slag can be utilized efficiently and environmentally friendly.

The invention discloses an ore prospecting method for a carbonate rock type lead-zinc deposit. The ore prospecting method comprises the steps of: field screening, indoor screening, rare earth geochemical index and carbon-oxygen isotope geochemical index recognition, ore prospecting geochemical index system establishment, abnormal graph drawing to guide ore prospecting and the like. The method canbe used for rapidly guiding prospecting prediction of carbonate rock type lead-zinc ore deposit to improve the prospecting efficiency, also can be used for judging the ore-forming fluid migration pathof the carbonate rock type lead-zinc deposit and indicating a prospecting direction, and has very high application and popularization value. According to the method, the problems of long period, highinvestment and high risk in the existing exploration technology are solved, the economical efficiency and high efficiency of carbonate rock type lead-zinc deposit potential evaluation and ore right decision are realized, meanwhile, the method is also one of the important ways for realizing green exploration, and the ecological environment problem possibly caused in the implementation process of the traditional exploration technology is solved.

The invention discloses a method for rapidly evaluating a hybrid rice variety in a field, comprising the following steps: S1, dividing field performance and comprehensive agronomic traits of a hybrid rice variety and a hybrid combination into productivity, growing situation feature, maturing performance and grain shape and phase; setting relative weight of each trait; S2, rating the traits for rice collected from a field, including: S21, selecting a control variety; and S22, with the control variety as a reference, comparing and scoring the collected rice and the control variety, a score for the rice better than the control variety in traits being higher than a medium level and otherwise being lower than the medium level; S3, calculating a comprehensive score according to the set weights and the scores. The field evaluation and breeding for hybrid rice varieties and hybrid combinations is more technical, field screening for variety and hybrid combination breeding is never complex, random or uncontrollable, and the method belongs to a great modification on the breeding of hybrid rice varieties and hybrid combinations and has a promising application prospect.

The invention discloses a method for detecting the content of neomycin B (Neomycin B) in milk and an application thereof. The present invention provides a complete set of nucleic acid aptamers for detecting neomycin B, consisting of nucleic acid aptamer NEO1 and nucleic acid aptamer NEO2; the nucleotide sequence of the nucleic acid aptamer NEO1 is sequence 1; the The nucleotide sequence of the nucleic acid aptamer NEO2 is sequence 2. Experiments of the present invention have proved that the invention overcomes the deficiencies in the prior art and provides a method for detecting neomycin B (Neomycin B) content in milk; Control and raw milk stations are used for on-site screening in large quantities.

The invention provides a doctor-patient communication emergency nursing platform, which comprises a patient client, a patient real-name-authenticated account, a mobile phone client, a network client, an online consulting module, a key field screening module, a central processor, a registration integrated processing module, a network registration module, an outpatient service registration module, a doctor client, a doctor real-name-authenticated account, a comprehensive scoring module, a personal information module and a patient evaluation module, wherein the patient client is connected with the doctor client through the key field screening module and the central processor in sequence. The doctor-patient communication emergency nursing platform has the beneficial effects of allowing doctors and patients to communicate before face-to-face diagnosis and treatment, and making full use of the first-aid time.

The invention belongs to the field of plant tissue culture and breeding and discloses a developing method of melon haploid. The method includes the steps of A, deactivating melon pollen; B, using pollen radiation to perform pollination on melon female flowers which bloom on the current day; C, selecting ovaries 11-12 days after the pollination, taking out immature embryos, and culturing the immature embryos in an MS culture medium; D, picking out green embryos, and culturing the green embryos in an induction culture medium; E, after buds grow, using a flow cytometer to perform ploidy identification on leaves; F, performing induction rooting on plants, which are identified as the haploid, in a rooting culture medium to obtain haploid plants. The method has the advantages that the method is applicable to domestic thin-peel melon varieties; the cultured haploid plants can be directly used as breeding materials after field screening, and the method is applicable to melon variety breeding and can accelerate melon breeding.

The invention provides a method and device for detection of nitrite in food and belongs to the field of food safety detection. The device comprises an extraction bottle used for holding a food sample, a mixing needle used for fully mixing and crushing the food sample in the extraction bottle, a burette used for dripping water into the extraction bottle holding the mixed and crushed food sample, a test strip used for being immersed into a food sample water solution to detect the content of nitrite, and a test strip tube used for holding the test strip, wherein a color chart is arranged on the outer wall of the test strip tube and used for comparing the test strip socked in the food sample water solution; and the unit of the corresponding nitrite contents marked on the color chart is the international unit. Through adding purified water to the sample, rapid field screening detection of nitrite can be realized conveniently and the method and the device can be extensively used in the field of food safety detection.

The invention discloses a continuously integrated respiratory infection virus self-detection kit, which comprises a sampling tube and a splitting-amplifying tube, wherein one end of the sampling tubeis provided with an opening, and the other end is sealed through a sieve membrane; an absorption filtration carrier is arranged in the sampling tube; and a buffer solution for splitting media, nucleicacid amplification media and nucleic acid fluorescent dyes is pre-loaded in the splitting-amplifying tube. The invention also discloses application of the kit, the kit is applied to the detection ofrespiratory infection viruses, and a detected person can finish continuously integrated respiratory infection virus self-detection on the own. The aerosol of the nasal cavity can be directly collectedto finish nucleic acid extraction and enrichment, nucleic acid purification and amplification and nucleic acid visual detection in an aerosol sample in one step, operation programs are greatly simplified, minimum duration for obtaining two visual detection results or above is 15 min, and therefore, the kit disclosed by the invention is especially suitable for the safe and quick field screening ofvirus nucleic acid in places, including customs, epidemic area checkpoints and the like.

The invention relates to a portable glaucoma rapid-screening analyzer which is characterized by mainly comprising an industrial computer, an LED brightness control device, a view field stimulation screen, a camera monitoring device, a responder and a printer, wherein the industrial computer is respectively connected with the LED brightness control device, the camera monitoring device, the responder and the printer; and the LED brightness control device is sequentially connected with a light-splitting device and the view field stimulation screen. The invention carries out static visual field examination in four specific positions of eyeballs in the way of four-point visual field examination, that is to say, the invention carries out threshold estimation and view field screening on four points by statically showing cursors with different brightness, thereby achieving the purpose of early treatment and diagnosis of glaucoma; in addition, the invention has the advantages of high accuracy, easy and convenient operation, short examination time consumption, high reliability and portability, is beneficial to the view field general survey of national people and the integral development of the ophthalmology field of national laser medicine and enhances the national visual field defect detection level.

The invention provides a method for screening transgenic radish in field by using a kanamycin leaf smearing method. The experimental material is transgenic radish of neomycin phosphotransferase II (NPT II)-labeled genes, the optimal concentration of the field screening of Kan leaf smearing is 1,000mg / L, and the observation time is preferably 12 days; and verified by polymerase chain reaction (PCR) detection, the positive detection rate of Kan screened resistance seedlings reaches 93.8 percent. According to the invention, the simple and convenient method for screening a large amount of transgenic radish in field is researched and established, and has important value on breading of the transgenic radish material.

The invention belongs to the technical field of drying and screening equipment for rubber seals, and relates to an all-in-one machine for drying and screening rubber seals. A heater is arranged at the bottom of a rectangular box. Sieve, the lower end of the sieve part of the shaking sieve is provided with a discharge port extending out of the box, the upper part of the box body on the high side of the sieve part of the shaking sieve is provided with a feeding port, and the upper part of the box is provided with more than one upper cover , The high-end side of the sieve part of the sieve is equipped with a drive mechanism that drives the sieve to reciprocate. The advantages are: one piece of equipment can replace the original two pieces of equipment for drying and screening, with high production efficiency, small footprint and low cost. , Energy saving and power saving, reducing labor intensity, suitable for drying and screening rubber seals after grinding and cleaning.

The invention relates to the technical field of medical examination, in particular to a quantum dot joint detection test strip for influenza viruses A and B as well as a preparation method and application of the quantum dot joint detection test strip. The invention discloses a joint detection test strip taking quantum dots as markers and capable of simultaneously detecting influenza viruses A and B. The joint detection test strip comprises a bottom plate, and a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad which are sequentially overlapped on the bottom plate from left to right, the combination pad is coated with an influenza virus type A detection antibody marked by quantum dots and an influenza virus type B detection antibody marked by quantum dots. According to the invention, the quantum dot is used as a marker, is easy to couple with antibody molecules, and is high in fluorescence intensity, strong in stability and not easy to attenuate after excitation, so that the detection has higher sensitivity, and early screening of influenza virus infection is facilitated. The joint detection test strip can be prepared into a strip-shaped structure by being combined with an immunochromatography technology, and is suitable for field screening and bedside detection by being matched with a handheld exciter.

The invention discloses an ore prospecting method for a Carlin type gold deposit. The ore prospecting method comprises the steps of: field screening, indoor screening, rare earth geochemical index andcarbon-oxygen isotope geochemical index recognition, ore prospecting geochemical index system establishment, abnormal graph drawing to guide ore prospecting and the like. The method can be used for rapidly guiding Carlin-type gold deposit prospecting prediction, improves the prospecting efficiency, also can be used for judging the ore-forming fluid migration path of Carlin-type gold deposit and indicating a prospecting direction, has very high popularization and application value, solves the problems of long period, high investment and high risk of existing exploration technology, realizes the economical efficiency and high efficiency of Carlin-type gold deposit potential evaluation and ore right decision, is also one of important ways for realizing green exploration, and solves the ecological environment problem possibly caused in the traditional exploration technology implementation process.

The invention discloses a method and device for managing and controlling spatial data in a customized range, and the method comprises the steps: building a first incidence relation between authority level information and authority range coordinates, building a second incidence relation between a user and the authority level information, and obtaining a request format of a request under the condition that a target user requests a spatial data map service; and determining a request type based on the request format, allocating a target permission processor based on the request type, and obtaining target spatial data in a permission range corresponding to the request based on the target permission processor and the second association relationship. The spatial data is managed and controlled by establishing the incidence relation between the permission level information and the user and permission range coordinates, a token mechanism and a field screening mode are not adopted any more to control permissions, slicing services and image data do not need to be segmented, the problems of inconvenience in client maintenance and token updating, data fragmentation and redundancy are avoided, and the user experience is improved. And the cost of data production, updating and maintenance is reduced.