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Use of ion mobility spectrometer for detecting gibberellins residues in crops and method for detecting gibberellins residues in crops

An ion mobility spectrometer and ion mobility spectrometry technology, which is applied to instruments, measuring devices, scientific instruments, etc., can solve the problems of complicated operation, cumbersome operation, unsuitable for gibberellin determination, etc., and achieve high detection sensitivity and simple operation method. , the effect of easy screening and detection

Inactive Publication Date: 2014-08-27
WUHAN SYSCAN TECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Using fluorescence spectrophotometry and ultraviolet spectrophotometry to determine gibberellin has the disadvantages that although the sensitivity is high, their accuracy is easily reduced by the interference of impurities, and the detection limit is low, which is not suitable for relatively large components. Determination of Gibberellins in Complex Samples
[0011] The disadvantages of the immunoassay method are: the preparation of antibodies is relatively complicated, the requirements for experimental conditions are high, and false positive results are prone to occur
[0016] The disadvantages of the above chromatographic methods are: the pretreatment is cumbersome, and more chemical reagents and equipment are used; the chromatographic ultraviolet and chromatographic mass spectrometers are expensive, and the maintenance cost is high; High professional knowledge requirements, time-consuming and labor-intensive
[0020] The disadvantages of thin layer chromatography are: cumbersome operation, not only need to use a large amount of organic solvents, but also only preliminary qualitative and semi-quantitative

Method used

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  • Use of ion mobility spectrometer for detecting gibberellins residues in crops and method for detecting gibberellins residues in crops
  • Use of ion mobility spectrometer for detecting gibberellins residues in crops and method for detecting gibberellins residues in crops
  • Use of ion mobility spectrometer for detecting gibberellins residues in crops and method for detecting gibberellins residues in crops

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Embodiment Construction

[0045] Below, the present invention will be described in detail with reference to the accompanying drawings.

[0046] Such as figure 1 As shown, the present embodiment provides a method for rapid detection of gibberellin residues in crops, the method comprising:

[0047] Prepare a gibberellin standard use solution with a certain concentration gradient, and put it into an ion mobility spectrometer to obtain the ion mobility spectrogram of the standard use solution and its gibberellin standard curve;

[0048] Prepare a sample solution of the crop to be tested, and put it into an ion mobility spectrometer to obtain an ion mobility spectrogram of the sample solution;

[0049] The ion mobility spectrogram of the sample solution is compared with the standard spectrum of the ion mobility spectrum of the standard liquid for use, and the actual content of gibberellin in the sample solution is calculated according to the standard curve of the ion mobility spectrum of the standard liqui...

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Abstract

The invention provides the use of an ion mobility spectrometer for detecting gibberellins residues in crops and a method for detecting the gibberellins residues in the crops. The uses of the ion mobility spectrometer are expanded, the thought of detecting gibberellins by using the ion mobility spectrometer is disclosed for the first time, and an implementation method for detecting the gibberellins by using the ion mobility spectrometer is provided. The gibberellins are quickly and conveniently detected, the operating method is simple, the detection sensitivity is high, and the detection result accuracy is high. The gibberellins can be detected in a laboratory as well as a supermarket and a farmers market, and law enforcement officials can implement field screening and detection conveniently.

Description

technical field [0001] The invention relates to the technical field of ion mobility spectrometer and agricultural residue detection, in particular to the use and method of an ion mobility spectrometer for detecting gibberellin residues in crops. Background technique [0002] Gibberellins (Gibberellins, Gas) are a class of plant hormones belonging to diterpenoids, which widely exist in higher plants, fungi and bacteria, and participate in the regulation of various stages of plant growth and development. At present, more than 100 kinds of gibberellins have been isolated from higher plants and microorganisms, generally exist in two forms of free and bound, named GA1, GA2...GAn, etc., collectively referred to as gibberellins. Among them, gibberellic acid GA3 (Gibberellic acid) is the most active, widely distributed, and has been studied the most, so it is often used as a representative of gibberellin. Gibberellin GA3, also known as 920, scientific name is 2,4a,7-trihydroxy-1-me...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/62G01N27/622
Inventor 张伟颜毅坚王智琪徐翔马军刘鹏
Owner WUHAN SYSCAN TECH CO LTD
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