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Screening method for selenium-enriched rice variety

A screening method and variety technology, applied in the field of agricultural germplasm resources screening, can solve the problems of long cycle and low accuracy, and achieve the effects of improving accuracy, accurate screening results, and simple preparation

Active Publication Date: 2018-11-06
INST OF AGRI PROD QUALITY SAFETY & STANDARD JIANGXI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Therefore, the technical problem to be solved in the present invention is to overcome the defects of low accuracy and long cycle in the screening process of rice germplasm resources in the prior art, thereby providing a screening method for selenium-enriched rice varieties

Method used

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  • Screening method for selenium-enriched rice variety
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  • Screening method for selenium-enriched rice variety

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The present embodiment provides a screening method for selenium-enriched rice varieties, comprising the following specific steps:

[0023] 1) Select the seeds of the rice varieties to be screened, which are plump and healthy, such as Huanghuazhan, Zhongzao 35, IR19735, Yangdao 6, Wuyanjing, and Xiangwanxian 12, and use 70 % ethanol for 2 minutes, then 2% sodium hypochlorite for 35 minutes, rinsed with sterile water for 4 times, germinated at 30°C, and the seeds were sown in petri dishes with selenium-containing medium in advance, and each petri dish was sown with 100 grain.

[0024] Among them, the selenium-containing medium is composed of the following components: sodium selenite 1.5mg / L, fulvic acid 0.1mg / L, agar 8g / L, prepared with sterile distilled water.

[0025] 2) Place the petri dish in a plant light incubator with a humidity of 70-90% and a daytime light intensity of 100-120 μmol / m 2 S, the light time is 10 hours, the daytime temperature is 30-32°C, and the n...

Embodiment 2

[0031] The present embodiment provides a screening method for selenium-enriched rice varieties, comprising the following specific steps:

[0032] 1) Select the seeds of the rice varieties to be screened, which are plump and healthy Juembo 1, Xiangwanxian 13, Heishai, Minghui 63, Meixiangxinzhan, Zaonuo, Ganxiangzhan, and the seeds of Henan black indica glutinous rice As a control, sterilize with 75% ethanol for 1 minute, then sterilize the seeds with 3% sodium hypochlorite for 25 minutes, rinse with sterile water for 5 times, germinate at 28°C, and sow the seeds in a petri dish with selenium-containing medium beforehand. Sow 120 grains in a petri dish.

[0033] Among them, the selenium-containing medium is composed of the following components: sodium selenite 0.1mg / L, fulvic acid 1mg / L, agar 6g / L, prepared with sterile distilled water.

[0034] 2) Place the petri dish in a plant light incubator with a humidity of 70-90% and a daytime light intensity of 100-120 μmol / m 2 S, th...

Embodiment 3

[0040] The present embodiment provides a screening method for selenium-enriched rice varieties, comprising the following specific steps:

[0041] 1) Select the seeds of rice varieties to be screened that are plump and healthy, such as Shuhui 527, R216, Heizhan 96, Jintai Ruanzhan, Heishai, Chunhui 350, and Chishi, and use Henan Heixiannuo as a control, and use 73 % ethanol for 2 minutes, then sterilized with 2.5% sodium hypochlorite for 30 minutes, rinsed with sterile water for 5 times, germinated at 29°C, and the seeds were sown in petri dishes with selenium-containing medium in advance, and each petri dish was sown with 110 grain.

[0042]Among them, the selenium-containing medium is composed of the following components: sodium selenite 1mg / L, fulvic acid 0.5mg / L, agar 7g / L, prepared with sterile distilled water.

[0043] 2) Place the petri dish in a plant light incubator with a humidity of 70-90% and a daytime light intensity of 100-120 μmol / m 2 S, the light time is 11 ho...

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Abstract

The invention belongs to the technical field of screening of agricultural germplasm resources, and particularly relates to a screening method for a selenium-enriched rice variety. According to the method, preliminary screening is performed at first through indoor cultivation, so that the blindness of the subsequent field screening test is reduced; South Henan black glutinous rice is used as a contrast material, so that the screening process is simple and feasible. In the indoor screening process, a selenium-enriched culture medium is used to provide a uniform and stable selenium source for rice seedlings; the accuracy of variety screening can be substantially improved; seeds are started to be cultivated in the initial stage of germination and growth; nutrition is mainly supplied by the seeds to the seedlings; the culture medium is relatively easy to prepare, and the screening method is easy to apply.

Description

technical field [0001] The invention belongs to the technical field of screening agricultural germplasm resources, and in particular relates to a screening method for selenium-enriched rice varieties. Background technique [0002] Selenium (Se), as an important trace element in the ecological environment, has various functions such as anti-aging, anti-cancer and detoxification, and has been designated by the World Health Organization and the Chinese Medical Association as a must-supplement after iodine and zinc in the 21st century. The third largest micronutrient health care element. The safe selenium intake recommended by the Health Institute of the Chinese Center for Preventive Medicine is 40-240 μg / person / day. According to the survey report, the current selenium intake of adults is about 26.63 μg / day, so it is necessary to further increase the selenium intake. [0003] Rice is the main food crop in my country. It is an ideal way to supplement selenium by increasing the s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/04
CPCA01H1/04
Inventor 张标金史华新陈庆隆戴廷灿周瑶敏魏益华
Owner INST OF AGRI PROD QUALITY SAFETY & STANDARD JIANGXI ACAD OF AGRI SCI
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