The invention discloses a decayed-sample degradation DNA (deoxyribonucleic acid) detection method. The method includes the steps of a), acquiring DNA, located at a nucleosome core region, of human blood tissue; b), sequencing the DNA at the nucleosome core region; c), comparing a sequence acquired by sequencing with human genome sequence information to acquire SNVs (single nucleotide variants), Indels or STRs genetic markers by screening; d), according to the sequences of the SNVs, the Indels or the STRs genetic markers, designing upstream or downstream primers; e), extracting the DNA of decayed samples; f), performing PCR amplification to the DNA of decayed samples through the upstream or downstream primers to obtain amplification products; and g), performing electrophoresis on the amplification products and performing type analysis according to genetic loca acquired from amplification. The decayed-sample degradation DNA is detected by the aid of the genetic markers of the nucleosome core region, the detection rate of the genetic loca is higher, acquired detection information is more comprehensive, and more accurate and reliable results can be acquired from medicolegal expertise and archaeological analysis.