Method for separating metabolizable cephalotaxus sinensis alkaloid strains

A technology for alkaloids and Torreya sinensis, which is applied in the field of separating metabolizable Torreya sinensis alkaloid strains, can solve the problems of low yield of Torreya sinensis alkaloids, difficult to meet living needs, slow growth of tree species, etc., so as to shorten the time required for screening, Screening results are efficient and metabolically active

Inactive Publication Date: 2015-02-18
LUOYANG INST OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, crude torreya resources are scarce, and the tree species grows slowly, and the yield of crude torreya alkaloids is low, which is difficult to meet people's living needs, and also causes the expensive price of crude torreya alkaloid drugs.

Method used

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  • Method for separating metabolizable cephalotaxus sinensis alkaloid strains
  • Method for separating metabolizable cephalotaxus sinensis alkaloid strains

Examples

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Embodiment 1

[0020] A method for isolating metabolizable crude torreya alkaloid strains, comprising the following steps:

[0021] Step 1. Selection and processing of explants

[0022] Choose the root, stem and leaf of perennial thick torreya as explant, clean the explant with running water, then cut the root into 5-6cm long pieces, remove the old bark and branches and leaves on the stem, and set aside;

[0023] Step 2, sterilization of explants

[0024] Put the treated explants into the ultra-clean workbench, first sterilize them with alcohol with a volume concentration of 75% for 5 minutes, then sterilize them with mercuric chloride with a mass concentration of 0.1% for 12 minutes, wash them with sterile water for 4 times, and put The processed explants were put into sterile water and set aside;

[0025] Step 3, purification and isolation of crude Torreya strain

[0026] Put the sterilized explants into the culture bottles containing the PDA medium respectively, place the culture bottl...

Embodiment 2

[0036] A method for isolating metabolizable crude torreya alkaloid strains, comprising the following steps:

[0037] Step 1. Selection and processing of explants

[0038] Choose the root, stem and leaf of perennial thick torreya as explant, clean the explant with running water, then cut the root into 5-6cm long pieces, remove the old bark and branches and leaves on the stem, and set aside;

[0039] Step 2, sterilization of explants

[0040] Put the treated explants into the ultra-clean workbench, first sterilize them with alcohol with a volume concentration of 75% for 5 minutes, then sterilize them with mercuric chloride with a mass concentration of 0.1% for 12 minutes, wash them with sterile water for 5 times, and put The processed explants were put into sterile water and set aside;

[0041] Step 3, purification and isolation of crude Torreya strain

[0042] Put the sterilized explants into the culture bottles containing the PDA medium respectively, place the culture bottl...

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Abstract

The invention belongs to the field of plant endophyte separation and microbial fermentation engineering, and particularly relates to a method for separating metabolizable cephalotaxus sinensis alkaloid strains. According to the method, roots, stems and leaves of perennial cephalotaxus sinensis are selected as explant; the explant is cut to strips, sterilized and then placed into a culture medium for culturing so as to produce cephalotaxus sinensis symbiotic bacteria; and then the cephalotaxus sinensis symbiotic bacteria are separated according to different strains and cultured and fermented respectively, and the cephalotaxus sinensis symbiotic bacteria are detected after fermentation broth is concentrated so as to determine whether the cephalotaxus sinensis symbiotic bacteria are metabolizable cephalotaxus sinensis alkaloid strains. In a fermentation broth preparation process, second batch of strains are inoculated after metabolic organisms are generated by the strains, the metabolic organisms generated by the previous batch of strains provide nutrients for the second batch of strains, so that growth of the second batch of strains is promoted greatly, the biological activity is improved simultaneously, a large amount of fermentation broth is generated accordingly, and the accuracy is improved while strain screening is facilitated.

Description

technical field [0001] The invention belongs to the field of plant endophytic bacteria isolation and microbial fermentation engineering, and in particular relates to a method for isolating metabolizable crude torreya alkaloid strains. Background technique [0002] Torreya is a unique plant in my country, which has high nutritional value and economic value. Torreya alkaloid is one of the important active ingredients of Torreya, which has anti-cancer effects and is often used in medicine. At present, crude torreya resources are scarce, and the tree species grows slowly, and the yield of crude torreya alkaloids is low, which is difficult to meet people's living needs, and also causes high prices of drugs related to crude torreya alkaloids. Therefore, increasing the yield of crude torreya alkaloids has become a long-standing desire of people. [0003] Biological fermentation engineering is a new comprehensive applied discipline that emerged in the early 1970s. The large-scale p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00
CPCC12N1/14
Inventor 王祖华王安亭李永程杨瑞先黄向东王佳伟
Owner LUOYANG INST OF SCI & TECH
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