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One-step rapid detection method of ochratoxin A by using electrochemical aptamer sensor

An ochratoxin, nucleic acid aptamer technology, applied in the direction of electrochemical variables of materials, can solve the problems of long detection time, complex detection scheme, and high detection cost, and achieve low detection cost, reduced detection cost, and high detection sensitivity. Effect

Inactive Publication Date: 2012-10-17
HEFEI UNIV OF TECH
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Problems solved by technology

[0004] In order to solve the shortcomings of the existing detection cost of ochratoxin A, the detection scheme is complicated, and the detection time is too long, the present invention provides a method for rapidly detecting ochratoxin A in one step using an electrochemical nucleic acid aptamer sensor

Method used

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  • One-step rapid detection method of ochratoxin A by using electrochemical aptamer sensor

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Experimental program
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Embodiment

[0027] (1) Polishing treatment of bare gold electrode surface

[0028] The bare gold electrode was ground and polished in an aqueous solution of alumina powder with a concentration of 0.3 mmol / L for 5 min, then polished in an aqueous solution of alumina powder with a concentration of 0.05 mol / L for 5 min, and then ultrasonically cleaned in an anhydrous ethanol solution 5 minutes, and finally ultrasonic cleaning in deionized water for 5 minutes to completely remove the alumina powder non-specifically adsorbed on the surface of the bare gold electrode;

[0029] (2) Modification of aptamer electrochemical sensors:

[0030]Take 2 mL of tris(β-chloroethyl)phosphate with a concentration of 1 mmol / L, add it to 198 mL of ochratoxin A nucleic acid aptamer solution with a concentration of 1 mmol / L, activate and incubate at room temperature for 30 min, and obtain Activated ochratoxin A nucleic acid aptamer solution; take 12 mL of activated ochratoxin A nucleic acid aptamer solution with...

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Abstract

The invention relates to a one-step rapid detection method of ochratoxin A by using an electrochemical aptamer sensor, comprising the following steps: (1)polishing the surface of a bare gold electrode; (2)modifying the electrochemical aptamer sensor; (3) detecting an ochratoxin A standard concentration solution, and establishing a standard curve; and (4) carrying out quantitative determination on an actual sample solution containing ochratoxin A. According to the invention, the sensitivity of detecting ochratoxin is raised, the minimum detectable amount of ochratoxin A is 0.01 pg / mL, the cost of detecting the target toxin is greatly reduced, the operation is simple and convenient, and the one-step detection of ochratoxin A can be realized in 5-10 min.

Description

technical field [0001] The invention belongs to the technical field of food detection, and in particular relates to a detection method of ochratoxin A in food. Background technique [0002] Ochratoxin A widely exists in nature, its toxicity is strong, and it is extremely harmful to humans, animals and plants. Ochratoxin A is produced by a variety of Aspergillus and Penicillium that grow on crops such as grains (wheat, corn, barley, oats, rye, rice, and millet, etc.), peanuts, and vegetables (beans). The toxin may also be present in the muscle tissue of pigs and hens, among others, after animals have ingested moldy feed. Ochratoxin A mainly damages the liver and kidney of animals. This toxin mainly causes kidney damage, and a large amount of toxin may also cause intestinal mucosal inflammation and necrosis in animals. Its teratogenic effects have been observed in animal experiments. [0003] Ochratoxin A is highly toxic and widely exists in various foods, grains and their...

Claims

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Application Information

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IPC IPC(8): G01N27/48
Inventor 陈伟吴晶晶薛峰梅占龙张睿陈寒清蒋原
Owner HEFEI UNIV OF TECH
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