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Method for preparing egg albumin polypeptide from egg albumin powder by enzymic method

A technology of ovalbumin and protein powder, which is applied in the direction of fermentation, etc., can solve the problems of difficult production process, low peptide yield, unsuitable for filtration, etc., achieve low production cost, high peptide yield, and improve technological content and quality.

Inactive Publication Date: 2013-03-20
山东天智绿业生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, ovalbumin polypeptides are generally obtained directly by enzymatic hydrolysis of ovalbumin solution. The production process is difficult and takes a long time. It is not suitable for filtration and the peptide yield is low.

Method used

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Embodiment

[0017] Its process steps are:

[0018] Egg protein powder→adding water to adjust slurry→heating to adjust pH value→adding enzyme to hydrolyze→heating to deactivate enzyme→filtering→vacuum concentration→low temperature drying→ovalbumin polypeptide.

[0019] The specific operation steps are:

[0020] (1) Dissolve 5kg egg protein powder in 10kg deionized water and keep stirring;

[0021] (2) Take 10g of papain (enzyme activity 1 million u / g), add 10kg of deionized water, dissolve it and add it to the feed liquid, stir well and mix evenly; during this process, stir at high temperature, the temperature is controlled at 40 degrees plus or minus 1 degrees, PH at 7±1 for 8 hours;

[0022] (3) After 8 hours of hydrolysis, the reaction is stopped, and the enzymatic hydrolysis is completed, and at the same time, the temperature is raised to a boiling state for 5 minutes to fully inactivate;

[0023] (4) After inactivating the enzyme, filter with a 0.3um membrane to remove the albumin ...

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PUM

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Abstract

The invention relates to the field of production of high-purity egg albumin polypeptide, and especially discloses a method for preparing egg albumin polypeptide from egg albumin powder by an enzymic method. According to the method for preparing egg albumin polypeptide from egg albumin powder by an enzymic method, the egg albumin powder is used as raw material; and the method provided by the invention is characterized by comprising the following steps of: dissolving the egg albumin powder in deionized water, adding a papain solution for hydrolysis, then, heating up the solution to be boiling, preserving heat and deactivating enzyme; executing membrane filtration to collect filtrate from the solution treated by enzyme deactivation; concentrating the filtrate in a vacuum low-temperature triple effect concentrating pan, and then, adding the active carbon, heating up, preserving heat and executing frame filtering, and carrying out spray drying for the filtrate to get the finished product. The method provided by the invention is simple in steps, low in production cost, exhaustive in enzymolysis, short in reaction time, easy to filter, high in peptide yield and capable of greatly improving the technological content and the quality of products, and being used and popularized widely.

Description

(1) Technical field [0001] The invention relates to the field of high-purity ovalbumin polypeptide production, in particular to a method for enzymatically preparing ovalbumin polypeptide from egg protein powder. (2) Background technology [0002] Protein peptide is a kind of biologically active substance, which is a mixed oligopeptide with certain biological functions obtained by proteolysis of protein or microbial fermentation. From a chemical point of view, protein peptide is a general term for different peptides ranging from dipeptides to complex linear and circular structures composed of 20 natural amino acids in proteins in different compositions and arrangements. Biological tests have shown that most of the protein ingested by the body is absorbed in the form of low-molecular-weight peptides after the action of enzymes in the digestive tract, and a small proportion is absorbed in the form of free amino acids. Further experiments revealed that low-molecular-weight ...

Claims

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Application Information

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IPC IPC(8): C12P21/06
Inventor 张九勋李雷张学军张贵文张平张丽
Owner 山东天智绿业生物科技有限公司
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