Application of diacylglycerol kinase alpha gene-chitosan nanoparticle in preparing medicine for treating allergic asthma

Abstract

The invention discloses an application of a DGK alpha (diacylglycerol kinase alpha) gene-chitosan nanoparticle as a unique active component in preparing a medicine for treating allergic asthma as well as a preparation method of the DGK alpha gene-chitosan nanoparticle. The invention, aiming at shortcomings that naked DGK alpha plasmid is easy to be damaged by nuclease, acid, alkali and the like in the body, prepares the DGK alpha gene-chitosan nanoparticle through chitosan, so as to effectively protect the DGK alpha plasmid, enhance the activity of the DGK alpha plasmid in the body and promote the DGK alpha gene to develop better function in the body. The DGK alpha, based on a function of induction of T cell anergy, can induce immune tolerance. The DGK alpha gene-chitosan nanoparticle prepared by the invention can inhibit egg albumin-induced airway inflammation of asthmatic mice, reduce serum egg albumin specific IgE level, and is expected to be used for treating asthma and other allergic diseases.

Description

Technical field [0001] The invention belongs to the field of biomedical preparations, and particularly relates to the application of diglyceride kinase alpha (DGKα) gene-chitosan nanoparticles in the preparation of medicines for treating allergic asthma. Background technique [0002] Asthma is a chronic airway allergic inflammatory disease, and the excessive activation of CD4+T cells plays an important role in the pathogenesis of asthma. After activation of CD4+ T cells, the differentiation into Th2 cells increased, and the overactivation of Th2 cells resulted in Th2 response-based immune response, and increased secretion of Th2 cytokines IL-4, IL-5 and IL-13. , Eventually leading to the accumulation of inflammatory cells such as eosinophils in the airway, causing airway allergic inflammation. Studies have shown that after normal people are repeatedly exposed to allergens, the stimulation of the allergens will not cause excessive activation of antigen-specific T cells to initiat...

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Problems solved by technology

Sanjuan et al [Sanjuan MA, Jones DR, Izquierdo M, et al.Role of diacylglycerol kinase alpha in the attenuation of receptor signaling.J Cell Biol.2001:153,207-220.Sanjuan MA, Pradet-Balade B, Jones DR, et al. T cell activation in vivo targets diacylglycerol kinase to the membrane: a novel mechanism for Ras attenuation. J Immunol, 2003, 170: 2877-2883.] The study found that T cells transfected with DGKα gene overexpressed DGKα and inhibited diacylglycerol Ester-dependent T cell receptor (TCR) signal, thereby weakening Ras activation signal and inhibiting ERK phosphorylation, inhibiting the activity of IL-2 promoter, weakening the expression of T cell activation signal CD69, resulting in T cell anergy

At present, there are no related reports on the preparation of DGKα gene-chitosan nanoparticles, and reports on the application of chitosan-wrapped DGKα gene in the treatment of asthma

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