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Method for extracting and purifying salvianolic acid A

A purification method and technology of salvianolic acid, applied in the field of compound preparation, can solve the problems of low salvianolic acid A content, unsatisfactory satisfaction, high production cost, etc., and achieve the effects of low production cost, reasonable design and stable performance

Inactive Publication Date: 2008-02-27
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Due to the low content of salvianolic acid A in medicinal materials, the production cost is high
In addition, the application of column chromatography in large-scale industrial production has certain limitations, and cannot well meet the needs of industrial production of salvianolic acid A preparations

Method used

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  • Method for extracting and purifying salvianolic acid A

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The extraction and purification method of salvianolic acid A, its processing steps are:

[0022] 1. Take the medicinal material of Danshen, add 8 times the amount of water, decoct and reflux 3 times, 1 hour each time, filter, concentrate the filtrate under reduced pressure to 1ml equivalent to 2g of medicinal material, and filter;

[0023] 2. Add 5% gelatin aqueous solution until no precipitation occurs, and centrifuge at 4000 rpm for 10 minutes; add acetone aqueous solution equivalent to 50% of the volume of the concentrated solution in step 2 to the precipitation, and heat at 60°C to dissolve the precipitation; after decompression recovery to remove acetone, the aqueous solution Extract 3 times with an equal volume of ethyl acetate, and combine the organic phases;

[0024] 3. Concentrate the extract under reduced pressure to extract, and freeze-dry to obtain a light yellow powder, which is salvianolic acid A. See Figure 1 for the chromatogram of salvianolic acid A pu...

Embodiment 2

[0026] It is basically the same as Example 1, except that the extraction solvent is 50% aqueous ethanol.

Embodiment 3

[0028] It is basically the same as Example 1, except that the concentrated solution in step 1 is concentrated to 1 ml which is equivalent to 0.5 g of medicinal materials.

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Abstract

The invention discloses an extracting and purifying method of salviol acid A, which comprises the following steps: adopting salvia miltiorrhizae medicine materical crude slice or powder as raw material; using solvent to extract; condensing to obtain the condensate of salvia miltiorrhizae; obtaining high-purity product through protein adsorption and solvent extracting method; using gelatin or egg albumin to sediment salviol acid A; dissolving the salviol acid A through acetone solution; analyzing; extracting through organic solvent to obtain the high-purity product with receiving rate over 0. 3% and purity over 90%. The invention has advantages of reasonable design, high extraction rate, good purity, stable property, simple technique and low manufacturing cost without column chromatography, which is fit for industrial manufacturing.

Description

technical field [0001] The invention belongs to compound preparation, and mainly relates to a method for extracting and purifying salvianolic acid A. Background technique [0002] Salvia miltiorrhiza is one of the most commonly used Chinese medicinal herbs. For the treatment of cardiovascular and cerebrovascular diseases such as angina pectoris, hypertension, coronary heart disease and stroke. There are many traditional Chinese medicine preparations that use Danshen decoction pieces or crude extracts of Danshen as medicine, such as compound Danshen tablets, compound Danshen dripping pills, Danshen injection, Xiangdan injection, etc. In addition, there are also pure compounds or their salts prepared from Danshen Prepared preparations, such as Danshensu Sodium Injection, Salvianolic Acid B Injection, etc. [0003] In recent years, with the deepening of pharmacological research, various pharmacological activities of salvianolic acid A have been discovered. For example, Zhen ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C69/732C07C67/48A61K36/53
Inventor 瞿海斌程翼宇徐金钟
Owner ZHEJIANG UNIV
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