Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for two-aqueous phase extraction of abalone egg albumin polypeptide concentrated liquor

A technology of egg protein polypeptide and two-phase water, which is applied in the field of separation and purification of biologically active substances, can solve problems such as waste of abalone nutritional resources, and achieve the effects of saving extraction time, strong binding, and short phase separation time

Active Publication Date: 2014-06-11
辽宁阿里郎生物工程股份有限公司
View PDF3 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional abalone processing method removes abalone roe as waste, resulting in a waste of abalone nutritional resources

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for two-aqueous phase extraction of abalone egg albumin polypeptide concentrated liquor
  • Method for two-aqueous phase extraction of abalone egg albumin polypeptide concentrated liquor
  • Method for two-aqueous phase extraction of abalone egg albumin polypeptide concentrated liquor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Take 150kg of fresh abalone eggs and clean them with edible water, then use a stainless steel colloid mill to crush the abalone eggs, input them into a high-shear emulsifier (manufactured by Wenzhou Haichuan Machinery Co., Ltd.), add 150kg of edible water, and stir evenly. 300kg of abalone roe homogenate was produced by high-shear emulsification at 3000r / min, and filtered through 100 mesh to obtain 300kg of abalone roe homogenate with a concentration of 18% for later use. Choose polyethylene glycol (PEG8000) 21.6kg, drinking water (H 2 O) 98.4kg, using a 600L stainless steel compound tank (manufactured by Wenzhou Haichuan Machinery Co., Ltd.) to stir and mix evenly to make {A} the upper phase of the first group of ligands is 120kg, and the solute (mass fraction) concentration is 18%. Then select NaCl, 19.2kg., H 2 O100.8kg. Prepare {A} first group of ligand lower phase 120kg, solute (mass fraction) concentration 16%. Start the thick slurry pump (manufactured by Wenzh...

Embodiment 2

[0048] Embodiment 2 differs from Embodiment 1 in that:

[0049] 1. Abalone roe homogenate;

[0050] Prefabricated abalone roe homogenate, choose frozen abalone roe 100kg water 73.6%, protein content 23.3% as raw material. After cleaning, crush, add 100kg of pure water according to the mass ratio of abalone roe, and make 200kg of homogenate through a 3000r / min high-shear emulsifier (manufactured by Wenzhou Haichuan Machinery Co., Ltd.), and filter through an 80-mesh filter (manufactured by Wenzhou Haichuan Machinery Co., Ltd. ) The pass rate was 85.8%, the homogenate concentration (mass fraction) was required to be 22%, and 171.6kg of abalone roe homogenate was obtained for use.

[0051] 2. Aqueous ligand;

[0052] Select the sum of abalone egg protein polypeptide extraction ligands to be 429kg and distribute as follows;

[0053] 171.6kg of extracted ligands in Group A accounted for 40% of the total ligands;

[0054] Upper ligand: dextran (DX48) 10.3kg purified water (H 2 ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Viscosityaaaaaaaaaa
Viscosityaaaaaaaaaa
Login to View More

Abstract

The invention relates to a biological active substance separating and purifying technology, and specifically relates to a method for two-aqueous phase extraction of abalone egg albumin polypeptide concentrated liquor. The method specifically comprises the following steps: prepreparing abalone egg homogenate, separating and purifying after two-aqueous phase continuous extraction, wherein total density of a two-aqueous phase system is 0.06kg / m<3>-0.16kg / m<3>, viscosity is 4mpa / s-16mpa / s, pH value is 6-8, extraction time is 30 minutes-50 minutes, and the feeding proportion of the total amount of the two-aqueous phase system to the abalone egg homogenate is 1: (0.4-0.5). The method disclosed by the invention is short in phase separating time, saves extraction time, saves energy consumption, and recycles distribution base liquor after phase separation, does not have organic solvent residue; moreover, the abalone egg albumin polypeptide concentrated liquor and nutritious foods are widely combined and applied.

Description

Technical field: [0001] The invention relates to separation and purification technology of biologically active substances, in particular to a method for extracting abalone egg protein polypeptide concentrated liquid with two aqueous phases. Background technique: [0002] Abalone eggs are rich in protein polypeptides, polysaccharides, nucleic acids, and gonadal hormones, which have anti-oxidation properties, remove excessive free radicals, and are biologically active substances that delay aging and relieve fatigue. The nutritional and functional substances of abalone are scientifically divided into: "One egg; that is, abalone roe; two dings; that is, abalone meat; important part. However, traditional abalone processing methods remove abalone roe as waste, resulting in a waste of abalone nutritional resources. After studying the active and effective ingredients in abalone roe, how to preserve abalone roe during the processing of abalone, and effectively extract the active ab...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07K1/14
Inventor 李长青郝家武洪明海郝明
Owner 辽宁阿里郎生物工程股份有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com