41 results about "Porcine muscle" patented technology

The invention relates to a SNP molecular marker affecting pig muscle fiber diameter and application thereof. The SNP site of the SNP molecular marker affecting pig muscle fiber diameter corresponds to the 69991162th base polymorphism site on chromosome 4 of the international pig reference genome Sscrofa11.1 version from the 5' end. The present invention also provides a primer for identifying the above-mentioned SNP molecular marker. Through the molecular marker and the primer, an efficient and accurate molecular marker-assisted breeding technique can be established. By optimizing the dominant allele of the SNP molecular marker, pig muscle fibers can be selected. The genetic progress of density traits can effectively improve the economic benefits of pig breeding.

The invention discloses a UCP3 gene, a molecular marker and its application related to pig intramuscular fat traits. The SNP molecular marker is a G / T mutation located at 2159bp of the sequence shown in SEQ ID NO: 6, and its genome position Located at site 8387309 from the 5' end of chromosome 9 of pig version 11.1. In the present invention, a mutation site that affects pig intramuscular fat content was identified in the gene by using the FastTarget target region sequencing technology in 279 test pigs, and a PCR-RsaI-RFLP gene rapid analysis for this site was established. type method; on this basis, the present invention has verified the application effect of improving intramuscular fat at this site in 500 test pigs, and the results show that the selection of favorable genotypes by this site can significantly improve the intramuscular fat of pigs. Fat content, the identified variant sites can be used for early, in vivo and rapid marker-assisted breeding of pig intramuscular fat.

The invention discloses an sgRNA (Subgnomic Ribonucleic Acid) for specific recognition of a porcine IGF2 (Lnsulin-like growth factors-2) gene intron and an encoding DNA (Deoxyribose Nucleic Acid) and application of sgRNA for the specific recognition of the porcine IGF2 gene intron. The invention provides a complete set of sgRNA which comprises an sgRNA-L-1 and an sgRNA-R-2; in the sgRNA-L-1, the nucleotide sequence for identifying fragments of a target sequence is used as the sequence I; in the sgRNA-R-2, the nucleotide sequence for identifying fragments of a target sequence is used as the sequence II. The sgRNA for the specific recognition of the porcine IGF2 gene intron disclosed by the invention can be used for knocking out a ZBED6 factor binding region in the IGF2 gene intron, so that the development of pig muscle cells can be promoted and the muscle content of a pig can be increased.

This application belongs to the field of genetic engineering application and biotechnology. The present invention provides a modified skeletal muscle-specific promoter 2eMCK-pMCK-T208, the nucleotide sequence of which is shown in SEQ ID NO: 1 in the sequence table. After connecting it with the target gene, it was found that the expression level of the target protein activated by 2eMCK‑pMCK‑T208 in the myocardium was lower than that of 2eMCK‑pMCK through intraperitoneal injection, showing a significant difference, while in skeletal muscle There was no significant difference in the expression level of the target protein initiated by 2eMCK-pMCK. The promoter disclosed in the present invention can enable high-efficiency and specific expression of foreign genes in skeletal muscle, which can not only improve pig muscle quality, but also reduce potential harm caused by low specificity of the promoter.

The invention discloses a method for identifying the diameter of pig muscle fibers and a pair of primers used therefor. A pair of primers for amplifying a DNA fragment containing the SSC4 g.69991162A>G polymorphic site; the SSC4 g.69991162A>G polymorphic site is from chromosome 4 of the pig reference genome Sscrofa11.1 from 5 The 69991162nd nucleotide from the 'end; the pig reference genome Sscrofa11.1 is the pig reference genome sequence updated in February 2017 in GenBank. The invention can carry out early screening of pigs to be selected, effectively alleviates the problem of long time for selecting good breeding pigs in actual production, reduces breeding costs, and effectively reduces or increases the diameter of pig muscle fibers. The method has high accuracy, low detection cost, and can Realizing automatic detection has high practical application value in pig breeding.

The invention belongs to the technical field of molecular markers, particularly relates to a molecular marker related to pig muscle fiber area and intramuscular fat content, a detection method and application of the molecular marker, and aims to obtain a molecular marker related to pig muscle fiber area and intramuscular fat content, and establish a method for determining the pig muscle fiber areaand intramuscular fat content within a short time, thereby determining meat tenderness, and assisting in breeding. The technical scheme is as follows: a specific primer is designed according to the INDEL mutation site of the TNNC2 gene on the first exon, amplification is carried out, and the polymorphism detection is carried out on the site by using restriction endonuclease BsmAI, thus the size of the muscle fiber area and the intramuscular fat content between the pig individuals are distinguished according to the polymorphism detection results.

The invention discloses a lncRNA marker related to porcine muscle fiber types and an application thereof, and belongs to the technical field of livestock molecular marker preparation. The lncRNA marker is LincRNA-ROMT, and its sequence is shown in SEQ ID NO: 1. The LincRNA‑ROMT is closely related to the type of porcine muscle fibers, and its expression is extremely low in oxidized porcine muscle fibers (fatty Lantang pig muscle tissue); it can be applied to the process of assisted genetic selection in pig breed improvement. The LincRNA-ROMT disclosed in the invention can be used as a molecular marker related to the type of porcine muscle fibers, and has important practical application value in genetically assisted breeding.

The invention relates to a SNP molecular marker affecting porcine muscle fiber density and application thereof. The SNP site of the SNP molecular marker affecting the porcine muscle fiber density corresponds to the 19658063 bp from the 5' end on chromosome 6 of the international pig reference genome Sscrofa11.1 version. The present invention also provides a primer for identifying the above-mentioned SNP molecular marker. Through the molecular marker and the primer, an efficient and accurate molecular marker-assisted breeding technique can be established. By optimizing the dominant allele of the SNP molecular marker, pig muscle fibers can be selected. The genetic progress of density traits can effectively improve the economic benefits of pig breeding.

The invention belongs to the technical field of screening and application of porcine molecular markers, and in particular relates to a method for increasing porcine muscle mass. The invention utilizes interference and overexpression of the MN864465 gene to increase the muscle mass and muscle fiber density of pigs. The MN864465 gene is amplified from the large white pig genome, and its nucleotide sequence is shown in SEQ ID NO: 1, which is located in the 104,826,111-104,827,563 region of pig chromosome 8. Two vectors were constructed to overexpress and interfere with the gene in porcine skeletal muscle satellite cells respectively. It was found that the gene can promote the proliferation of porcine skeletal muscle satellite cells and inhibit the differentiation of porcine skeletal muscle satellite cells, that is, it can increase pig muscle mass and muscle fiber density . Injection of pig and mouse leg muscles with overexpressed lentivirus of this gene can inhibit muscle growth and development. The present invention provides a new resource for pig muscle improvement.