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41 results about "Porcine muscle" patented technology

Porcine smooth muscle cells are an economical alternative suitable for investigations in cardiac disease and arteriosclerosis, especially in co-culture with species-matched porcine arterial endothelial cells. Proliferation of the smooth muscle cells is considered a key event in the development...

Application of long-chain non-coding RNA IncMGPF in regulation and control of muscle development functions of pigs

The invention belongs to the technical field of animal genetic engineering, and particularly relates to application of long-chain non-coding RNA IncMGPF in regulation and control of muscle developmentfunctions of pigs. An over-expression vector and interfere vector of long-chain non-coding RNA IncMGPF are constructed, skeletal muscle satellite cells of the pigs are infected at a cellular level ina slow virus infection manner, biceps femoris muscles of the pigs are infected at a living level and are collected after being infected by viruses for multiple times, and the influences of IncMGPF onthe growth and development of the muscles are proved according to the individual pig, so that a technical basis is laid for the cultivation of new species of pork-type pigs with higher yield.
Owner:HUAZHONG AGRI UNIV

Bovine lactoferricin-human lysozyme fused protein, gene and application thereof

The invention discloses bovine lactoferricin-human lysozyme fused protein. The bovine lactoferricin-human lysozyme fused protein is characterized by comprising porcine myofibrillar protein antioxidative peptide, bovine lactoferricin, hen egg white protein antioxidative peptide, porcine muscle myosin antioxidative peptide, flexible peptide and human lysozyme which are connected in series. Through anionic antioxidative peptide derived from fusion expression animals, the positive charge of antimicrobial peptide is neutralized, and inhibition to host bacteria by antimicrobial peptide is reduced; antioxidative peptide is beneficial to the improvement of storage stability of the antimicrobial peptide, the antibacterial activity to escherichia coli (ATCC 25922) by the fused protein is only reduced by 7.7 percent after the fused protein is laid for 20d at 4 DEG C, and the loss of activity to the escherichia coli (ATCC 25922) by the fused protein is only reduced by 15.4 after the fused proteinis laid for 30d.
Owner:ZHEJIANG UNIV OF TECH

Separation and purification method of high-purity porcine muscle stem cells

The invention relates to a separation and purification method of high-purity porcine muscle stem cells, for research and production of cultured meat. The separation and purification method disclosed by the invention comprises the steps of obtaining sterile muscular tissue, efficiently digesting the muscular tissue to obtain a large quantity of muscle mononuclear cells, and finally performing steaming cell sorting to obtain the high-purity porcine muscle stem cells. The invention further discloses a method for efficiently digesting the porcine muscular tissue. By the method, the digestion speedand the digestion intensity can be increased, and a large quantity of muscle mononuclear cells can be obtained. When the purification method disclosed by the invention is applied, the porcine musclestem cells of which the purity exceeds 92% (by PAX7) can be stably obtained. The porcine muscle stem cells obtained through separation have efficient disintegration efficiency, and MYOSIN expression quantity can reach 85%-90%. When the method disclosed by the invention is applied, a large quantity of seed cell sources can be provided for research and actual production of the cultured meat.
Owner:NANJING AGRICULTURAL UNIVERSITY

Molecular marker related to pig muscle pH value character and application thereof

The invention belongs to the technical field of preparation of a pig genetic marker, and particularly relates to a preparation method of a pig muscle pH value related molecular marker and application of the pig muscle pH value related molecular marker on auxiliary marker selection. The molecular marker is obtained by cloning a GYS1 gene promoter sequence, and the nucleotide sequence of the molecular marker is as shown in the sequence table SEQ ID NO: 1; and a C267-T267 basic group on the 267th basic group of a sequence as shown in the sequence table SEQ ID NO: 2 is mutated to cause MvaIPCR-RFLP polymorphism. The invention also discloses application of the preparation method for obtaining the molecular marker and a polymorphism detection method. The preparation method disclosed by the invention provides a new genetic marker for carrying out the auxiliary marker selection of a high-muscle quality pig.
Owner:SICHUAN AGRI UNIV

Molecular marker related to pH (Potential of Hydrogen) value character of pig muscle

The invention belongs to the technical field of preparation of livestock molecular markers, and particularly relates to an SNP (Single Nucleotide Polymorphism) molecular marker related to a pH (Potential of Hydrogen) value character of pig muscle. The molecular marker is obtained by LDHA (Lactate DehydrogenaseHydroxyacyl) gene cloning and has a nucleotide sequence as shown in Figure 4.In the sequence as shown in Figure 4, the 495th position has allele G or A base substitution, and the 635th position of the sequence has allele C or T base substitution. The new molecular marker is provided for assistant selection of pig markers.
Owner:HUAZHONG AGRI UNIV

Method for increasing muscle mass of pigs

The invention belongs to the technical field of screening and application of molecular markers of pigs and specifically relates to a method for increasing muscle mass of the pigs. The method disclosedby the invention increases the muscle mass and muscle fiber density of the pigs through interference and over-expression of an MN864465 gene. The MN864465 gene is obtained through amplification in agenome of large white pigs, wherein a nucleotide sequence of the gene is shown in SEQ ID NO:1, and the sequence is located in areas 104, 826, 111-104, 827 and 563 in a No.8 chromosome of the pigs. Twocarriers are obtained through construction, the gene is over-expressed and intervened in a porcine skeletal muscle satellite cell respectively, and the gene is found to promote proliferation of the porcine skeletal muscle satellite cell and restrain differentiation of the porcine skeletal muscle satellite cell, namely the muscle mass and muscle fiber density of the pigs can be increased. Growth and development of muscle can be restrained during lentivirus injection of leg muscle of the pigs and mice with over-expression of the gene. The method provided by the invention provides new resource for muscle improvement of the pigs.
Owner:HUAZHONG AGRI UNIV

Feed additive for promoting growth of porcine skeletal muscle and application thereof

The invention discloses a feed additive for promoting growth of porcine skeletal muscle and application thereof in the technical fields of feed processing and fish breeding and poultry raising. The feed additive is a carnosine obtained from condensation of beta-alanine and L-histidine with a relative molecular weight of 226.24, and is a natural antioxidant. The feed additive is capable of activating an mTOR signal pathway and enhancing proliferation of porcine skeletal muscle satellite cells, thus promoting the growth of the porcine skeletal muscle. The mechanism of porcine muscle growth is explained in terms of cells and an important idea is offered for development of animal growth promoting additives. When the additive is added to a pig feed, the growth performance of pigs and the meat producing efficiency can be improved. The additive has remarkable economic benefits, is safe without any risk even in long-term use, and has the characteristics of going green, safety, and environmental protection.
Owner:CHINA AGRI UNIV

LncRNA marker related to porcine muscle fiber types and application of lncRNA marker

The invention discloses an lncRNA marker related to porcine muscle fiber types and an application of the lncRNA marker, and belongs to the technical field of livestock molecular marker preparation. The lncRNA marker is LincRNA-ROMT, and the sequence of the lncRNA marker is as shown in SEQ ID NO:1. The LincRNA-ROMT is closely related to the porcine muscle fiber types, and extremely remarkably showslow expression in oxidized porcine muscle fibers (fat type Lantang porcine muscle tissues). The lncRNA marker can be applied to an auxiliary genetic breeding process in the aspect of pig variety improvement. The LincRNA-ROMT disclosed by the invention can be used as a molecular marker related to porcine muscle fiber types, and has important practical application values in genetic assisted breeding.
Owner:SOUTH CHINA AGRI UNIV

Pig muscle quality correlated numerator mark CSRP3 clone and application thereof

The invention belongs to the livestock gene engineering technical field, in particular relating to a molecular marker GSRP3 and an application thereof, wherein, the molecular marker GSRP3 is applied as porcine marker-assisted selection and relates to quality properties of porcine muscle. The molecular marker is produced by cloning a CSRP3 gene, and a cDNA sequence of the molecular marker is shown in a sequence table SEQ ID NO: 1. A base substitution of C1924-T1924 exists at 1924bpth of a sequence table SEQ ID NO: 2, and leads to the enzyme digestion polymorphism of TaqI PCR-RFLP. The invention also discloses a primer used for amplifying an intact cDNA sequence and a partial DNA sequence of the CSRP3 gene and a method for polymorphic detection, and provides a novel molecular marker for the porcine marker-assisted selection.
Owner:HUAZHONG AGRI UNIV

Method for detecting content of triglyceride in porcine muscle with gene PPARG

The invention discloses a method for detecting the content of triglyceride in porcine muscle with gene PPARG. The expression level of gene PPARG in adipose tissue is utilized to detect the content of triglyceride in porcine muscle. The method has the characteristics of rapidity, accuracy, convenient operation and no influence to the normal growth of pigs, is suitable for dynamic monitoring of the content of triglyceride in porcine muscle during pig breeding so as to grasp the situation of pig growth at any time, can achieve relatively accurate understanding of the content of fat in porcine muscle, and then adjusts the daily ration of pigs.
Owner:GUANGXI UNIV

Construction method, identification method and application of porcine FLJ small interference RNA expression vector

The invention discloses a construction method of a porcine FLJ small interference RNA expression vector. The construction method comprises the following steps of: 1) designing 3 pairs of siRNA primers of loop rings containing 9 nucleotides, wherein the 9 nucleotides are TTCAAGAGA; and 2) constructing an RNAi expression vector, namely after annealing the siRNA primers, connecting the siRNA primers with double-enzyme-cut RNAi vector and converting to obtain recombinant plasmid of the RNAi expression vector. The invention also provides a method for identifying the constructed porcine FLJ effective small interference RNA expression vector. The invention further provides application of the porcine FLJ effective small interference RNA expression vector constructed by the method. The porcine FLJ effective small interference RNA expression vector is used for researching the influence of the FLJ gene on fat deposition in porcine muscle.
Owner:ZHEJIANG UNIV

A kind of bovine lactoferrin peptide-human lysozyme fusion protein, gene and application thereof

The invention discloses bovine lactoferricin-human lysozyme fused protein. The bovine lactoferricin-human lysozyme fused protein is characterized by comprising porcine myofibrillar protein antioxidative peptide, bovine lactoferricin, hen egg white protein antioxidative peptide, porcine muscle myosin antioxidative peptide, flexible peptide and human lysozyme which are connected in series. Through anionic antioxidative peptide derived from fusion expression animals, the positive charge of antimicrobial peptide is neutralized, and inhibition to host bacteria by antimicrobial peptide is reduced; antioxidative peptide is beneficial to the improvement of storage stability of the antimicrobial peptide, the antibacterial activity to escherichia coli (ATCC 25922) by the fused protein is only reduced by 7.7 percent after the fused protein is laid for 20d at 4 DEG C, and the loss of activity to the escherichia coli (ATCC 25922) by the fused protein is only reduced by 15.4 after the fused proteinis laid for 30d.
Owner:ZHEJIANG UNIV OF TECH

Marker Lnc-482286 related to porcine skeletal muscle satellite cell proliferation, and application of marker Lnc-482286

The invention discloses a long non-coding RNA marker Lnc-482286 related to porcine skeletal muscle satellite cell proliferation, and application of the long non-coding RNA marker Lnc-482286, and belongs to the technical field of molecular biology. The long non-coding RNA marker is Lnc-482286, and the nucleotide sequence of the long non-coding RNA marker Lnc-482286 is shown as SEQ ID NO.1. The marker Lnc-482286 provided by the invention is highly expressed in porcine muscle tissues, expression of the Lnc-482286 in the muscle tissue of the Jinfen white pig is proved to be in a trend of firstly increasing and then decreasing along with the increase of the day age, and the Lnc-482286 plays a significant up-regulation role in the process of the porcine skeletal muscle satellite cell proliferation. The proliferation condition of the porcine skeletal muscle satellite cells can be identified by detecting the expression of the Lnc-482286, the porcine skeletal muscle satellite cell proliferation can be accelerated by improving the expression of the Lnc-482286, and a new thought and method is provided for clinical research on the growth and development of the porcine skeletal muscle.
Owner:SHANXI AGRI UNIV +1

Method for low-cost in-vitro culture of porcine muscle stem cells

The invention discloses a method for low-cost in-vitro culture of porcine muscle stem cells, and belongs to the technical field of stem cell and animal cell culture. The invention discloses a culturemedium and the culture method for culturing the muscle stem cells at low cost, the muscle stem cells can keep in-vitro growth and myogenic differentiation capacity through the culture medium and the culture method, and the cost of the culture medium is greatly reduced.
Owner:JIANGNAN UNIV

Construction and application of over-expression vectors of three subtypes of porcine PDLIM3 protein

The invention specifically relates to construction and application of over-expression vectors of three different subtypes of porcine PDLIM3 protein, belonging to the field of bioengineering technology. The nucleotide sequences of the gene coding regions of the three different subtypes of the porcine PDLIM3 protein are shown in SEQ ID No. 1, SEQ ID No. 2 and SEQ ID No. 3 in a sequence table. Three over-expression vectors of the three subtypes of the porcine PDLIM3 protein can be simultaneously constructed by subjecting the complete coding region fragments of the genes of the three subtypes of the porcine PDLIM3 protein to double enzyme restriction and connecting the treated fragments with the pEGFP-N1 vector having undergone double enzyme restriction. The above-mentioned eukaryotic expression vector construction method is simple and practicable; the over-expression vectors can highly-efficiently enhance expression of the PDLIM3 protein, all contain green fluorescent protein and can be used for detecting expression and location of the three different subtypes of the porcine PDLIM3 protein in eukaryotic cells. The over-expression vectors provided by the invention are also applicable to research on roles of the porcine PDLIM3 protein in growth and development of porcine muscle fibers.
Owner:SICHUAN AGRI UNIV

A kind of ucp3 gene related to pig intramuscular fat traits, molecular marker and application thereof

The invention discloses a UCP3 gene, a molecular marker and its application related to pig intramuscular fat traits. The SNP molecular marker is a G / T mutation located at 2159bp of the sequence shown in SEQ ID NO: 6, and its genome position Located at site 8387309 from the 5' end of chromosome 9 of pig version 11.1. In the present invention, a mutation site that affects pig intramuscular fat content was identified in the gene by using the FastTarget target region sequencing technology in 279 test pigs, and a PCR-RsaI-RFLP gene rapid analysis for this site was established. type method; on this basis, the present invention has verified the application effect of improving intramuscular fat at this site in 500 test pigs, and the results show that the selection of favorable genotypes by this site can significantly improve the intramuscular fat of pigs. Fat content, the identified variant sites can be used for early, in vivo and rapid marker-assisted breeding of pig intramuscular fat.
Owner:NANJING AGRICULTURAL UNIVERSITY +1

Primer group for real-time fluorescent quantitative PCR (Polymerase Chain Reaction) detection of porcine muscle regulatory factor and application of primer group

The invention discloses a primer group for real-time fluorescent quantitative PCR (Polymerase Chain Reaction) detection of a porcine muscle regulatory factor and application of the primer group, and belongs to the technical field of biology. The invention provides a specific primer pair capable of detecting the porcine muscle regulatory factors MyoD and MyoG and an internal reference primer pair,cDNA is obtained by extracting RNA of a sample and performing reverse transcription, and real-time fluorescent PCR is performed by using the provided primers. And therefore, mRNA expression quantitiesof the porcine muscle regulatory factors MyoD and MyoG can be rapidly, specifically, sensitively and stably detected at the same time; the primer group can be used for detecting the change of expression levels of the regulatory factors before and after differentiation of porcine muscle stem cells, and thus judges the differentiation condition of cultured pork.
Owner:CHINA MEAT RES CENT

Efficient and economical porcine muscle stem cell separation method and application thereof

ActiveCN114752590AEfficient and economical separation methodRaise the ratioCell dissociation methodsHydrolasesBiotechnologyMuscle tissue
The invention discloses an efficient and economical pig muscle stem cell separation method and application thereof, and belongs to the technical field of biology. According to the method, different enzymes for digesting muscle tissues are combined, the single cell releasing capacity of different combined enzymes is determined, the most efficient and economical enzyme combined digestion mode (the combination of streptavidin and dispersozyme) is selected, and finally a large number of muscle stem cells are obtained through a differential purification method. The invention further discloses a method for efficiently digesting the pig muscle tissue, and the muscle stem cells can be maximally added while the cost is reduced. By applying the enzyme combined digestion and separation method, the total single cell number of 4.6-6 * 10 < 6 > cells / g and the pig muscle stem cell number of 8-15 * 10 < 5 > cells / g (in a double positive proportion of CD29 and CD56) can be stably obtained. The muscle stem cells obtained through separation have high differentiation capacity, and an efficient and economical separation method is provided for research and actual production of cell culture meat seed cells.
Owner:JIANGNAN UNIV

Molecular marker related to pork quality character and application thereof

PendingCN114369669AAccelerate the improvement processImplement early selectionMicrobiological testing/measurementDNA/RNA fragmentationBiotechnologyNucleotide
The invention discloses a molecular marker related to pork quality characters and application of the molecular marker, and belongs to the field of molecular markers. The nucleotide of the molecular marker is shown as SEQ ID NO: 1, C / T mutation exists at the 302bp position of the sequence shown as SEQ ID NO: 1, the mutation comprises three mutation types of CC, CT and TT, and when the genotype of a pig individual is CC type, the contents of marbling of longissimus dorsi muscle, marbling of biceps femoris muscle and intramuscular fat of the longissimus dorsi muscle of the pig individual are higher than those of a CT type pig individual and a TT type pig individual. The invention further provides a primer pair for detecting the site, a corresponding detection method is established, and a new molecular marker is provided for genetic improvement of pig muscle quality. According to the present invention, the pork quality character can be subjected to early stage selection, and the detection method is efficient and accurate, such that the pork quality improvement process can be accelerated, and the economic benefits of pig breeding can be improved.
Owner:INST OF ANIMAL SCI & VETERINARY HUBEI ACADEMY OF AGRI SCI

Method for identifying diameters of muscle fibers of pig and primer pair used by method

ActiveCN110157816ASolve the problem that the living body cannot be measuredReduce breeding costsMicrobiological testing/measurementDNA/RNA fragmentationBiotechnologyFiber
The invention discloses a method for identifying diameters of muscle fibers of a pig and a primer pair used by the method. The primer pair amplifies a DNA fragment containing a polymorphism site whereSSC4 g. 69991162A is larger than G; the polymorphism site where the SSC4 g. 69991162A is larger than the G is the 69991162 nucleotide from the 5' terminal on the 4 chromosome of a porcine reference genome Sscrofa11.1; the porcine reference genome Sscrofa11.1 is a porcine reference genome sequence in GenBank, wherein the updated date is February, 2017. The method can perform early screening on to-be-selected pigs, so that the problem of long time for selecting good breeding pigs in actual production is effectively solved, the breeding cost is reduced, the diameters of the porcine muscle fibers are effectively reduced or increased, the method has high accuracy and low detection cost, automatic detection can also be achieved, and the practical application value in pig breeding is very high.
Owner:INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI

SNP (Single Nucleotide Polymorphism) molecular marker associated with drip loss character of porcine muscles and application of SNP molecular marker

ActiveCN114045350AImportant food valueImportant benefitsMicrobiological testing/measurementDNA/RNA fragmentationNucleotideVariome
Embodiments of the invention disclose an SNP molecular marker associated with drip loss of muscles of a Yorkshire pig and application of the SNP molecular marker. The SNP molecular marker is the polymorphic nucleotide site rs81470247 of an intron variant of the pig gene HOXA5; and the nucleotide sequences of upstream and downstream 100 bp of the site are as shown in SEQ ID No. 1, the 101 base of the sequence is mutated, and the base K is T or G, resulting in polymorphism. The invention further develops a primer for the SNP molecular marker. A method for screening a strain of a Yorkshire pig with low drip loss by identifying the SNP marker is established on the above basis, so breeding of the strain of the Yorkshire pig with low drip loss, breeding of high-quality pork or content detection is aided, and the pork has important edible value, economic benefits and social value.
Owner:HUAZHONG AGRI UNIV

Antibiotic-free crude feed suitable for free-range black pigs and application of antibiotic-free crude feed

The invention discloses an antibiotic-free crude feed suitable for free-range black pigs and an application of the antibiotic-free crude feed. The antibiotic-free crude feed is characterized by comprising the ration formula components and content of porcine muscle fat deposition after functional conversion. The antibiotic-free crude feed prepared by the method comprises the ration formula components and nutrient value, on one hand, the quality of meat of live pigs can be notably improved, and on the other hand, the nutrient transformation efficiency of the feed of variety pigs can be improved,and the discharge amount of nitrogen, phosphorus, copper and zinc in excrement can be greatly reduced.
Owner:TIANJIN AGRICULTURE COLLEGE
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