41 results about "Porcine muscle" patented technology

The invention discloses an sgRNA (Subgnomic Ribonucleic Acid) for specific recognition of a porcine IGF2 (Lnsulin-like growth factors-2) gene intron and an encoding DNA (Deoxyribose Nucleic Acid) and application of sgRNA for the specific recognition of the porcine IGF2 gene intron. The invention provides a complete set of sgRNA which comprises an sgRNA-L-1 and an sgRNA-R-2; in the sgRNA-L-1, the nucleotide sequence for identifying fragments of a target sequence is used as the sequence I; in the sgRNA-R-2, the nucleotide sequence for identifying fragments of a target sequence is used as the sequence II. The sgRNA for the specific recognition of the porcine IGF2 gene intron disclosed by the invention can be used for knocking out a ZBED6 factor binding region in the IGF2 gene intron, so that the development of pig muscle cells can be promoted and the muscle content of a pig can be increased.

The invention discloses a method for identifying the diameter of pig muscle fibers and a pair of primers used therefor. A pair of primers for amplifying a DNA fragment containing the SSC4 g.69991162A>G polymorphic site; the SSC4 g.69991162A>G polymorphic site is from chromosome 4 of the pig reference genome Sscrofa11.1 from 5 The 69991162nd nucleotide from the 'end; the pig reference genome Sscrofa11.1 is the pig reference genome sequence updated in February 2017 in GenBank. The invention can carry out early screening of pigs to be selected, effectively alleviates the problem of long time for selecting good breeding pigs in actual production, reduces breeding costs, and effectively reduces or increases the diameter of pig muscle fibers. The method has high accuracy, low detection cost, and can Realizing automatic detection has high practical application value in pig breeding.

The invention relates to a SNP molecular marker affecting porcine muscle fiber density and application thereof. The SNP site of the SNP molecular marker affecting the porcine muscle fiber density corresponds to the 19658063 bp from the 5' end on chromosome 6 of the international pig reference genome Sscrofa11.1 version. The present invention also provides a primer for identifying the above-mentioned SNP molecular marker. Through the molecular marker and the primer, an efficient and accurate molecular marker-assisted breeding technique can be established. By optimizing the dominant allele of the SNP molecular marker, pig muscle fibers can be selected. The genetic progress of density traits can effectively improve the economic benefits of pig breeding.

The invention belongs to the technical field of screening and application of porcine molecular markers, and in particular relates to a method for increasing porcine muscle mass. The invention utilizes interference and overexpression of the MN864465 gene to increase the muscle mass and muscle fiber density of pigs. The MN864465 gene is amplified from the large white pig genome, and its nucleotide sequence is shown in SEQ ID NO: 1, which is located in the 104,826,111-104,827,563 region of pig chromosome 8. Two vectors were constructed to overexpress and interfere with the gene in porcine skeletal muscle satellite cells respectively. It was found that the gene can promote the proliferation of porcine skeletal muscle satellite cells and inhibit the differentiation of porcine skeletal muscle satellite cells, that is, it can increase pig muscle mass and muscle fiber density . Injection of pig and mouse leg muscles with overexpressed lentivirus of this gene can inhibit muscle growth and development. The present invention provides a new resource for pig muscle improvement.