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A SNP molecular marker affecting porcine muscle fiber density and its application

A molecular marker, muscle fiber technology, used in recombinant DNA technology, microbial determination/inspection, DNA/RNA fragments, etc., can solve problems such as the difficulty of DNA detection, achieve convenient and efficient screening, improve detection efficiency, and solve the inability to live measured effect

Active Publication Date: 2022-04-26
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the prior art, there is also a location analysis of pork muscle fiber density QTL, and this trait QTL is located in the 139.1-153.9Mb interval on chromosome 6. There are more than 14 million DNA in this interval. Due to the large number of DNA As a result, it is very difficult to detect DNA variations in this interval
There are almost no reports of SNPs in this interval that are significantly associated with porcine muscle fiber density

Method used

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  • A SNP molecular marker affecting porcine muscle fiber density and its application
  • A SNP molecular marker affecting porcine muscle fiber density and its application
  • A SNP molecular marker affecting porcine muscle fiber density and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Embodiment 1, the muscle fiber density size of identification pig

[0030] 1. Determination of porcine SSC6g.19658063C>T polymorphism

[0031] (1) Two Damin hybrid pigs were used as experimental materials, and the genomic DNA of the ear margin tissues were extracted respectively.

[0032] (2) Design and synthesis of primers

[0033] According to the sequence of pig reference genome version 11.1, the following primers were designed and synthesized:

[0034] U (upstream primer): 5'-TGTCAACCAGAAGGCGTAAGTG-3'; as shown in SEQ ID No.1.

[0035] D (downstream primer): 5'-AAAGATAACAGCGGGCAAGGT-3'. As shown in SEQ ID No.2.

[0036] (3) PCR amplification

[0037] The genomic DNA of Damin hybrid pig obtained in step (1) was used as a template, and U and D were used as primers to carry out PCR amplification to obtain PCR amplification products, which were named product 1 and product 2, respectively.

[0038] PCR amplification system: Genomic DNA 200ng, 10×PCR amplification b...

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Abstract

The invention relates to a SNP molecular marker affecting porcine muscle fiber density and application thereof. The SNP site of the SNP molecular marker affecting the porcine muscle fiber density corresponds to the 19658063 bp from the 5' end on chromosome 6 of the international pig reference genome Sscrofa11.1 version. The present invention also provides a primer for identifying the above-mentioned SNP molecular marker. Through the molecular marker and the primer, an efficient and accurate molecular marker-assisted breeding technique can be established. By optimizing the dominant allele of the SNP molecular marker, pig muscle fibers can be selected. The genetic progress of density traits can effectively improve the economic benefits of pig breeding.

Description

technical field [0001] The invention relates to the technical field of biological breeding, in particular to provide a SNP molecular marker affecting the density of pig muscle fibers and its application. Background technique [0002] Pork quality is an important economic trait of pigs, and it is closely related to people's meat nutrition, meat processing and economic benefits of pig farming. Muscle fiber density, which is the main factor affecting pork quality, has also attracted more and more attention from researchers and breeders, and has become a part of pig breeding programs. [0003] Molecular breeding can carry out breeding work by screening molecular markers associated with traits to avoid slaughter. In the prior art, there is also a location analysis of pork muscle fiber density QTL, and this trait QTL is located in the 139.1-153.9Mb interval on chromosome 6. There are more than 14 million DNA in this interval. Due to the large number of DNA As a result, it is ver...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156C12Q2600/124
Inventor 张龙超王立贤王立刚刘欣
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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