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A method for identifying the diameter of pig muscle fiber and the pair of primers used therefor

A technique for muscle fibers and primer pairs, which is applied to a method for the diameter of muscle fibers and primer pairs used in the field, can solve problems such as difficulty in breeding work, achieve high practical application value, low detection costs, and reduce breeding costs.

Active Publication Date: 2022-04-26
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] However, the muscle fiber diameter can only be measured after slaughter, making breeding difficult

Method used

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  • A method for identifying the diameter of pig muscle fiber and the pair of primers used therefor
  • A method for identifying the diameter of pig muscle fiber and the pair of primers used therefor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1 identifies the muscle fiber diameter size of pig

[0040] 1. Determination of SSC4g.69991162A>G polymorphism in pigs

[0041] (1) Using two Beijing black pigs as experimental materials, the genomic DNA of the ear margin tissue was extracted respectively.

[0042] (2) Design and synthesis of primers

[0043] According to the sequence of pig reference genome 11.1, the following primers were designed and synthesized:

[0044] U (upstream primer): 5'-CAGCTCCATATTTCCTAAGTC-3' (shown in sequence 1);

[0045] D (downstream primer): 5'-GCAGAAGTTTCATAAGGCACATGAA-3' (shown in SEQ ID NO: 2).

[0046] (3) PCR amplification

[0047] The genomic DNA of the Beijing black pig obtained in step (1) was used as a template, and U and D were used as primers to carry out PCR amplification to obtain two PCR amplification products, which were named product 1 and product 2, respectively.

[0048] PCR amplification system: Genomic DNA 200ng, 10×PCR amplification buffer 5μl, dNT...

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Abstract

The invention discloses a method for identifying the diameter of pig muscle fibers and a pair of primers used therefor. A pair of primers for amplifying a DNA fragment containing the SSC4 g.69991162A>G polymorphic site; the SSC4 g.69991162A>G polymorphic site is from chromosome 4 of the pig reference genome Sscrofa11.1 from 5 The 69991162nd nucleotide from the 'end; the pig reference genome Sscrofa11.1 is the pig reference genome sequence updated in February 2017 in GenBank. The invention can carry out early screening of pigs to be selected, effectively alleviates the problem of long time for selecting good breeding pigs in actual production, reduces breeding costs, and effectively reduces or increases the diameter of pig muscle fibers. The method has high accuracy, low detection cost, and can Realizing automatic detection has high practical application value in pig breeding.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for identifying the diameter of pig muscle fibers and a pair of primers used therefor. Background technique [0002] Pork quality is an important economic trait of pigs, and it is closely related to people's meat nutrition, meat processing and economic benefits of pig farming. Therefore, the research on pork quality has become an important part in the fields of meat food processing and pig genetics and breeding. As the main factor affecting pork quality, the muscle fiber diameter has also attracted more and more attention from researchers and breeders, and has become a key component of pig breeding programs. part. [0003] However, the muscle fiber diameter can only be measured after slaughter, making breeding difficult. Molecular breeding can carry out breeding work by screening molecular markers associated with traits to avoid slaughter. Therefore, it is ver...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 张龙超王立贤王立刚刘欣
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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