The invention relates to the field of aquatic products, and discloses a biological inhibitor for
silver carp surimi gel degradation. The inhibitor is a
silver carp MBSP-specific shark-derived single-domain
antibody, myofibrillar-binding
serine protease (MBSP) which causes the
silver carp surimi gel degradation is used as an
antigen to immunize ginglymostoma cirratum, a single-domain
antibody geneis separated from ginglymostoma cirratum serum, a single-domain
antibody gene text
library is established, a single-domain antibody
target gene with high-specificity is obtained through the
phage display technology, and then the silver
carp MBSP-specific shark-derived single-domain antibody with high-purity, high-activity, and high-expression amount is separated and obtained by using the
cloning expression technology and
affinity chromatography technology. The biological inhibitor can significantly inhibit the activity of silver
carp myofibrillar-binding
serine protease, and effectively inhibits the gel degradation of silver
carp surimi. The biological inhibitor involved in the invention is safe, specific, and efficient, has simple operation during the use process, and can be applied to the inhibition of the gel degradation of silver carp surimi.