Myofibrillar protein-chitosan nanometer microsphere composite membrane
A myofibrillar protein and chitosan nanotechnology, which is applied in the field of food quality and safety, can solve the problems of ineffective use of minced meat and fish bones, restricting the further development of the industry, wasting fishery resources, etc. Peroxidation, significant bacteriostatic activity, value-added effect
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[0015] Embodiment: Myofibrillar protein-chitosan nano-microsphere composite film contains the following components in parts by weight: 20-22 parts of myofibrillar protein, 8-12 parts of gelatin plasticizer: 20-22 parts of chitosan Sugar microspheres, 0.4-0.6 parts of antioxidant.
[0016] Preparation of tuna minced myofibrillar protein: Take minced tuna, add Tris-Maleate buffer (0.05 mol / L, pH 7.5) according to the ratio of material to liquid 1 g:9 mL, homogenize at 18000 r / min, centrifuge at 6000 r / min for 12 min, take the precipitate and After repeating the homogenization-centrifugation twice, the obtained precipitate was cleaned by adding 0.1 mol / L NaCl solution according to the ratio of solid to liquid 1 g:4.5 mL, and centrifuged at 9000 r / min for 10 min, and the obtained precipitate was then mixed according to the ratio of solid to liquid 1 g:9 mL Add 0.1 mol / L NaCl solution to wash, filter with gauze, and centrifuge the filtrate to obtain a precipitate, which is the my...
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