The invention relates to the field of application of marine bioactive extracts, in particular to a method for preparing
tuna skin collagen peptide iron chelate. The method for preparing the
tuna skincollagen
peptide iron chelate comprises the steps that (1),
protein slurry is prepared, specifically,
tuna skin is crushed and subjected to homogenate with an acid solution, and then the
protein slurry is obtained after high-pressure treatment; (2) multiple
enzymatic hydrolysis is carried out, specifically, an
enzyme is added to the
protein slurry, the
enzyme is subjected to
enzymatic hydrolysis with heating and
enzyme deactivation at a high temperature to obtain an enzymatic
hydrolysate; (3) multiple
filtration is carried out, specifically, the enzymatic
hydrolysate is subjected to filtrationand
impurity removal, and then the enzymatic
hydrolysate is filtered through an organic membrane and subjected to
dialysis intercept, a retaining solution in a
dialysis bag is taken to obtain
a peptide solution; (4) a
chelation reaction is carried out, specifically, an acid is added to the
peptide solution to adjust the pH value to be acidic, and then
ferrous salt is added to perform the
chelation reaction, and then
centrifugation is carried out to obtain a layered solution; and (5) products are separated, specifically, supernatant of the layered solution is taken,
ethanol is added, and
precipitation is carried out at a low temperature, the
solid products are subjected to
centrifugal separation, the
solid products are dried and sterilized, and the tuna
skin collagen peptide iron chelate is obtained. The yield of the
peptide iron chelate is improved, and the method for preparing the tuna skin
collagen peptide iron chelate is simple and efficient.