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Minced tuna protein antioxidative peptide and preparation method and use thereof

An antioxidant peptide, tuna technology, which is applied in the preparation methods of peptides, chemical instruments and methods, food preparation and other directions, can solve the problems of the application effect of chemical synthesis antioxidants, and achieve the effects of no toxic side effects, high activity, and easy monitoring.

Active Publication Date: 2014-06-11
安徽盛美诺生物技术有限公司
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Problems solved by technology

Therefore, based on food safety considerations, the application of chemically synthesized antioxidants has been greatly affected

Method used

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  • Minced tuna protein antioxidative peptide and preparation method and use thereof
  • Minced tuna protein antioxidative peptide and preparation method and use thereof
  • Minced tuna protein antioxidative peptide and preparation method and use thereof

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Embodiment

[0032] Below in conjunction with embodiment the present invention is described in further detail.

[0033] Example: refer to Figure 1 to Figure 6 , a preparation method of tuna minced meat protein antioxidant peptide, the preparation process is as follows: tuna minced meat "defatting" enzymatic hydrolysis "enzymatic hydrolyzate" macroporous resin desalination "ultrafiltration" ion exchange chromatography "gel filtration chromatography "Preparation of" Antioxidant Peptides by High Performance Liquid Chromatography.

[0034] 1) Put the washed and removed tuna mince into phosphate buffer (pH 6.0) according to the solid-liquid ratio of 1 g: 1 mL, process it into a homogenate with a high-speed tissue masher, and then use a volume ratio of 1 : 3 Degrease in ether for 24 h, centrifuge at 5000 rpm for 15 min at 4°C to remove ether, collect the solids, which is defatted tuna mince;

[0035] 2) Add the defatted tuna mince into phosphate buffer (pH 6.0) according to the solid-to-liqui...

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Abstract

The invention discloses a minced tuna protein antioxidative peptide and its preparation method and use. The minced tuna protein antioxidative peptide has an amino acid sequence of Tyr-Glu-Asn-Gly-Gly. The preparation method comprises the following steps of adding a phosphate buffer solution into defatted minced tuna as a raw material, adjusting a pH value of the mixture to 5.0 to 7.0 to obtain a mixed solution, preheating the mixed solution with stirring, adding papain into the preheated mixed solution to obtain an enzymolysis product, carrying out enzyme deactivation of the enzymolysis product, and orderly carrying out desalination, ultrafiltration and chromatography to obtain the minced tuna protein antioxidative peptide. The preparation method has the advantages of scientific and reasonable processes and easy monitoring of enzymolysis. The minced tuna protein antioxidative peptide obtained by the preparation method has the advantages of safety, no toxic and side effect, strong antioxidant activity and easy digestive absorption, and can be used as a drug, a health-care food and a food additive.

Description

technical field [0001] The invention relates to a minced tuna protein antioxidant peptide, and also relates to a preparation method of the minced tuna protein antioxidant peptide, and also relates to a use of the minced tuna protein antioxidant peptide. Background technique [0002] Antioxidants are a class of substances that can prevent the adverse effects of oxygen, help capture and neutralize free radicals, thereby eliminating free radical damage to the human body and protecting food from oxidative damage and deterioration. Antioxidants can be divided into two types according to their sources: natural antioxidants and chemically synthesized antioxidants. Chemically synthesized antioxidants (such as butyl hydroxyanisole (BHA), dibutyl hydroxytoluene (BHT) and tert-butyl hydroquinone (TBHQ), etc.) are widely used in food because of their cheap price and good activity. In the industry, it can prevent or delay the oxidative deterioration of food, improve the stability of foo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/06C07K1/20C07K1/18C07K1/16C12P21/06A23L1/305A23L1/29A61K38/08A61P39/06A23L33/00
Inventor 王斌罗红宇李忠瑞胡发远
Owner 安徽盛美诺生物技术有限公司
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