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Method of preparing bioactive phospholipid from tuna eyes

A bioactive, tuna-based technology, applied in the field of food processing, can solve problems such as phospholipid research that has not been reported, and achieve the effect of enriching market space and promoting healthy development

Inactive Publication Date: 2018-12-25
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the utilization of tuna lipids is only used to prepare deep-sea fish oil, and the research on its phospholipids has not been reported

Method used

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  • Method of preparing bioactive phospholipid from tuna eyes
  • Method of preparing bioactive phospholipid from tuna eyes
  • Method of preparing bioactive phospholipid from tuna eyes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] This implementation case shows a method for preparing highly bioactive phospholipids from tuna eyes according to the following steps:

[0041]Take tuna eyes (10 times the diameter of tuna fish eyes), remove fish bones, add 95% alkaline ethanol solution (fish eye weight: solution volume is 1:5), homogenize at 4 °C for 15 minutes, in which 95% alkali The neutral ethanol solution is to add NaOH to 95% ethanol to make it pH 10.0. Under the action of ultrasonic waves, stir and extract the phospholipid-containing lipids in the eyes of tuna. The extraction time is 2 hours, and the extraction temperature is 40°C. During the extraction period, ultrasonic-assisted extraction is performed intermittently every 30 minutes. 60W / cm 2 , the ultrasonic frequency is 25kHz. After the extraction, filter with three layers of gauze and three layers of filter paper respectively, take the filtrate and distill it under reduced pressure at 40°C to collect the lipid fraction, and the ethanol ca...

Embodiment 2

[0043] This implementation case shows a method for preparing highly bioactive phospholipids from tuna eyes according to the following steps:

[0044] Take the tuna eye (12 times the diameter of the tuna fish eye), remove the fish bones, add 95% alkaline ethanol solution (fish eye weight: solution volume is 1:8), homogenize at 4 ° C for 18 minutes, in which 95% alkali The neutral ethanol solution is to add NaOH to 95% ethanol to make it pH 10.5. Under the action of ultrasonic waves, stir and extract the phospholipid-containing lipids in the eyes of tuna. The extraction time is 3 hours, and the extraction temperature is 50°C. During the extraction period, ultrasonic-assisted extraction is performed intermittently every 30 minutes. 80W / cm 2 , the ultrasonic frequency is 25kHz. After the extraction, filter with three layers of gauze and three layers of filter paper respectively, take the filtrate and distill it under reduced pressure at 42°C to collect the lipid fraction, and th...

Embodiment 3

[0046] This implementation case shows a method for preparing highly bioactive phospholipids from tuna eyes according to the following steps:

[0047] Take tuna eyes (15 times the diameter of tuna fish eyes), remove the fish bones, add 95% alkaline ethanol solution (fish eye weight: solution volume is 1:10), homogenize at 4 °C for 20 minutes, in which 95% alkali The neutral ethanol solution is to add NaOH to 95% ethanol to make it pH 11.0. Under the action of ultrasonic waves, stir and extract the phospholipid-containing lipids in the eyes of tuna. The extraction time is 5 hours, and the extraction temperature is 60°C. During the extraction period, ultrasonic-assisted extraction is performed intermittently every 30 minutes. 100W / cm 2 , the ultrasonic frequency is 25kHz. After the extraction, filter with three layers of gauze and three layers of filter paper respectively, take the filtrate and distill it under reduced pressure at 45°C to collect the lipid fraction, and the eth...

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Abstract

The invention discloses a method of preparing bioactive phospholipid from tuna eyes. The method includes: subjecting tuna eyes as a raw material to low temperature homogenization, extracting lipid oftuna eyes at room temperature by means of ultrasonic-assisted ethanol extraction, carrying out low-temperature low-pressure distillation to obtain a lipid component, subjecting the lipid to chromatography on silica gel columns, eluting with dichloromethane, acetone and methanol successively, collecting methanol eluate, and carrying out molecular distillation to obtain tuna eye active phospholipid.The method helps promote the benign development of the tuna processing industry and enrich the markets of marine bioactive lipids, and is positively significant to the human health and the development of marine health food industry.

Description

technical field [0001] The invention relates to the technical field of food processing, in particular to a method for preparing phospholipids with high biological activity from tuna eyes. Background technique [0002] Tuna is mainly distributed in the vast waters of the Pacific Ocean, the Atlantic Ocean and the Indian Ocean. It is a long-distance migratory pelagic economic fish. The annual tuna catch in my country is about 80,000 tons, and 50%-70% of the actual waste is produced according to processing. According to data calculation, there are about 40,000-56,000 tons of tuna scraps per year. Among these scraps, the eye part of tuna is the part with the highest lipid content, including a large amount of active phospholipid components. Phospholipids are rich in unsaturated fatty acids such as EPA and DHA. These phospholipids have good biological activity on improving memory and improving metabolic syndrome. [0003] At present, the utilization of tuna lipids is only used to...

Claims

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Application Information

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IPC IPC(8): C07F9/10
CPCC07F9/103
Inventor 胡世伟李世杰姜维刘宇
Owner ZHEJIANG OCEAN UNIV
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