Preparation method of tuna anserine

A technology of tuna and anserine, applied in the field of protein peptides, can solve the problems of wasting resources, not being environmentally friendly, and low efficiency of anserine, and achieve the effect of improving utilization rate, improving stability, and facilitating subsequent processing

Active Publication Date: 2020-04-14
GUANGDONG SHINYEE MARINE BIOLOGY ENG CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The preparation method of a kind of tuna anserine provided by the present invention overcomes the disadvantages of low efficiency of extracting anserine from traditional tuna, waste of resources and environmental protection

Method used

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  • Preparation method of tuna anserine
  • Preparation method of tuna anserine
  • Preparation method of tuna anserine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A preparation method for tuna anserine, comprising the steps of:

[0030] S1. Clean the tuna fish body, and remove the head, tail and viscera of the tuna fish.

[0031] S2. Clean the inside of the fish body, collect the cleaning solution, and filter it with a 300-mesh screen to obtain a filtrate.

[0032] S3. Crush the cleaned tuna body in step S2 and mix it with the filtrate obtained in step S2 to obtain a tuna slurry, which is then spray-dried to obtain 1 kg of crude dry powder.

[0033] S4. Mix 1kg of crude dry powder with 8kg of choline chloride deep eutectic solvent, stir at 50°C at 500rpm for 4h, cool to room temperature, then centrifuge at 3000rpm for 30min to obtain the first precipitate and collect it Serum. Wherein, the preparation of the choline chloride deep eutectic solvent comprises the steps of: mixing choline chloride and acetic acid in a molar ratio of 1:5 and stirring at 500 rpm for 3 hours at 60° C. to form a uniform transparent liquid at room tempe...

Embodiment 2

[0037] A preparation method for tuna anserine, comprising the steps of:

[0038] S1. Clean the tuna fish body, and remove the head, tail and viscera of the tuna fish.

[0039] S2. Clean the inside of the fish body, collect the cleaning solution, and filter it with a 300-mesh screen to obtain a filtrate.

[0040] S3. Crush the cleaned tuna body in step S2 and mix it with the filtrate obtained in step S2 to obtain a tuna slurry, which is then spray-dried to obtain 1 kg of crude dry powder.

[0041] S4. Mix 1kg of crude dry powder with 8kg of choline chloride deep eutectic solvent, stir at 50°C at 500rpm for 4h, cool to room temperature, then centrifuge at 3000rpm for 30min to obtain the first precipitate and collect it Serum. Wherein the preparation of the choline chloride deep eutectic solvent comprises the following steps: mixing choline chloride and lactic acid in a molar ratio of 1:5 and stirring at 500 rpm for 3 hours at 60°C to form a uniform transparent liquid, cooling ...

Embodiment 3

[0045] A preparation method for tuna anserine, comprising the steps of:

[0046] S1. Clean the tuna fish body, and remove the head, tail and viscera of the tuna fish.

[0047] S2. Clean the inside of the fish body, collect the cleaning solution, and filter it with a 300-mesh screen to obtain a filtrate.

[0048] S3. Crush the cleaned tuna body in step S2 and mix it with the filtrate obtained in step S2 to obtain a tuna slurry, which is then spray-dried to obtain 1 kg of crude dry powder.

[0049] S4. Mix 1kg of crude dry powder with 8kg of choline chloride deep eutectic solvent, stir at 50°C at 500rpm for 4h, cool to room temperature, then centrifuge at 3000rpm for 30min to obtain the first precipitate and collect it Serum. Wherein the preparation of the choline chloride deep eutectic solvent comprises the steps of: mixing choline chloride and glycerol in a molar ratio of 1:5 and stirring at 500 rpm for 3 hours at 60°C to form a uniform transparent liquid, cooling at room te...

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Abstract

The invention belongs to the technical field of protein peptides, and particularly relates to a tuna anserine preparation method which comprises the following steps: S1, cleaning tuna bodies; S2, cleaning the interior of the tuna bodies, and collecting filtrate; S3, preparing crude dry powder from tuna bodies and the filtrate; S4, mixing the crude dry powder with a choline chloride eutectic solvent to obtain a first precipitate, and collecting the supernate; S5, mixing the first precipitate with a detergent, and washing to obtain supernate; and S6, carrying out a mixing reaction on the supernate to obtain a second precipitate, mixing the second precipitate with a stabilizer solution, homogenizing, and drying to obtain powder namely the tuna anserine product. Anserine is extracted from tunaby using choline chloride eutectic solvents, so that the utilization rate of tuna is increased; and through the stabilizer, the stability of the tuna anserine is improved, and subsequent processing is facilitated, so that the tuna anserine product with high purity, high yield and high stability is obtained.

Description

technical field [0001] The invention belongs to the technical field of protein peptides, in particular to a preparation method of tuna anserine. Background technique [0002] Uric acid is a substance in human urine and is the end product of purine metabolism. The common abnormality of uric acid metabolism is high uric acid. When the purine substances in the human body are disturbed due to metabolism, the increase of uric acid in the blood will cause hyperuricemia, kidney stones, gout and other diseases. [0003] There are generally two ways to reduce uric acid. One is to control diet, fasting high-purine foods and drinking alcohol, but the effect is slow and only suitable for mild symptoms. The second is drug treatment, mainly colchicine, glucocorticoids, probenecid, allopurinol, benzbromarone, febuxostat and other drugs. These drugs take effect quickly and can relieve the pain in time, but they also have some unavoidable side effects, such as damage to the intestinal tra...

Claims

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Application Information

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IPC IPC(8): C07D233/64
CPCC07D233/64
Inventor 王炎冰刘扬龙秀红杨淑平
Owner GUANGDONG SHINYEE MARINE BIOLOGY ENG CORP
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