Method for preparing tuna skin collagen peptide iron chelate
A technology of iron chelate and tuna, which is applied to the preparation method of peptides, chemical instruments and methods, animal/human protein, etc., can solve the problems of cumbersome preparation, high cost, waste of resources, etc., achieve simple and efficient preparation, reduce equipment demand, the effect of simplifying the preparation process
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Embodiment 1
[0065] A kind of preparation method of tuna skin collagen peptide iron chelate, described preparation method comprises the following steps:
[0066] 1) Preparation of protein slurry: crush the tuna skin, add 5 times the loose volume of the crushed tuna skin in phosphate buffer for homogenization, and then obtain the protein slurry after high-pressure treatment at 400Mpa for 40 minutes;
[0067] 2) Multiple enzymatic hydrolysis: add active units to the protein slurry and step 1) the mass ratio of tuna skin used is 35000U: 1g of acid protease and active unit and step 1) the mass ratio of tuna skin used is 30000U: 1g pepsin , to form a pre-enzymolysis solution, control the pH value of the pre-enzymolysis solution to 5.0, heat up the pre-enzymolysis solution at 60°C for 6 hours, and perform ultrasonic-assisted enzymatic hydrolysis every 40 minutes, and the duration of a single ultrasonic-assisted enzymatic hydrolysis for 20 minutes, and then 95 ° C high temperature for 15 minutes ...
Embodiment 2
[0074] A kind of preparation method of tuna skin collagen peptide iron chelate, described preparation method comprises the following steps:
[0075] 1) Preparation of protein slurry: crush the tuna skin, add phosphate buffer solution 3 times the bulk volume of the broken tuna skin for homogenization, and then obtain the protein slurry after high-pressure treatment at 450Mpa for 40 minutes;
[0076] 2) Multiple enzymatic hydrolysis: add active units to the protein slurry and step 1) the mass ratio of tuna skin used is 45000U: 1g acid protease and active unit and step 1) the mass ratio of tuna skin used is 40000U: 1g pepsin , to form a pre-enzymolysis solution, control the pH value of the pre-enzymolysis solution to 4.5, heat up the pre-enzymolysis solution at 40°C for 8 hours, and perform ultrasonic-assisted enzymatic hydrolysis every 60 minutes, and the duration of a single ultrasonic-assisted enzymatic hydrolysis for 20 minutes, and then 100°C for 10 minutes to inactivate the...
Embodiment 3
[0082] A kind of preparation method of tuna skin collagen peptide iron chelate, described preparation method comprises the following steps:
[0083] 1) Preparation of protein slurry: crush the tuna skin, add phosphate buffer solution 4.5 times the bulk volume of the broken tuna skin for homogenization, and then obtain the protein slurry after high-pressure treatment at 350Mpa for 60 minutes;
[0084] 2) Multiple enzymatic hydrolysis: add active units to the protein slurry and step 1) the mass ratio of tuna skin used is 40000U: 1g of acid protease and active unit and step 1) the mass ratio of tuna skin used is 35000U: 1g pepsin , to form a pre-enzymatic hydrolysis solution, control the pH value of the pre-enzymolysis solution to 6.5, heat up the pre-enzymolysis solution at 50°C for 8 hours, and perform ultrasonic-assisted enzymatic hydrolysis every 60 minutes, and the duration of a single ultrasonic-assisted enzymatic hydrolysis for 20 minutes, followed by high temperature at 9...
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