121 results about "Animal Genetics" patented technology

The invention relates to an animal model for obese rats and an establishing method, and belongs to the field of animal genetic engineering and genetic modification. The animal model for obese rats and the establishing method are characterized in that rats with LEP and LEPR genes knocked out are obtained through the CRISPR/Cas9 technology. The rats with LEP and LEPR genes knocked out have a typical obesity characteristic, the reliable animal model can be provided for studying the obesity disease of people, and the rats with LEP and LEPR genes knocked out are expected to become a standard experiment animal of obese rats.

An Internet-based marketplace is provided for animals, gametes and embryos wherein a database retains specific genetic identifiers correlated to the animals, embryos and gametes. A method, an apparatus, a server and a client are provided that allow a remote user to submit a query on one or more genetic identifiers to a centralized database located on the server for the purpose of allowing a user to identify matching stock, to certify stock such as to reduce fraud, to report lost or found stock, to trade stock by either fixed priced selling or auction, and to facilitate shipment of stock. An animal can be identified and a cloned embryo prepared and shipped to the requester such as in a specialized shipping container that stabilizes the cloned embryo.

The invention belongs to the fields of molecular marker-assisted selection and animal genetic breeding and discloses an SNP (Single Nucleotide Polymorphism) molecular marker influencing pig eye muscleareas and application thereof. The SNP molecular marker refers to site mutation of No. 35091111 base A-G of chromosome 6 of pig genome, and corresponds to 214bp (named as g.214T>G) in a sequence shown as SEQ ID NO:1. By utilizing the primer pair in the invention, PCR (Polymerase Chain Reaction) amplification is performed by taking pig genome DNA as a template, so that the SNP molecular marker inclose linkage with the pig eye muscle area character can be obtained. The molecular genetic marker provided by the invention can be used for screening eye muscle areas of pigs, and the eye muscle areaand meat quality traits of the pigs can be effectively improved.

The invention belongs to the technical field of animal genetic engineering, and particularly relates to a growth hormone receptor gene taken as a Chinese grassland red bull excellent slaughter trait molecular marker and an application thereof. The length of a nucleotide sequence of an obtained GHR genetic segment is 489bp and the nucleotide sequence is a part of an exon. A base mutation exists at a 289-position basic group to cause the generation of restrictive fragment length polymorphism. The mutant site is taken as the molecular marker and detected by a PCR-RFLP (polymerase chain reaction-restrictive fragment length polymorphism) method using restriction enzyme BstACI, and detection results are associated with the Chinese grassland red bull slaughter traits so as to provide theoretical basis for selection breeding of Chinese grassland red bull. The invention is characterized in that partial segment of GHR gene associated with the Chinese grassland red bull excellent slaughter traits is obtained, and polymorphism analysis is conducted to the segment for providing the molecular marker for marker assisted selection of the Chinese grassland red bull.

The invention belongs to the technical field of manufacturing of animal genetic markers, and particularly relates to the technical field of single nucleotide polymorphism (SNP) detection at the 3908 bp of the third intron of the pig SLA-11 gene. A method for screening the genetic marker for character of litter size of the pig comprises the following steps: extracting a genome DNA from pig blood, designing a primer, performing PCR amplification, cloning PCR products, measuring sequence, comparing and analyzing the sequence, performing SNP detection, marking, conducting correlation analysis among the characters of litter sizes. According to the invention, the DNA sequence in the third intron zone of the pig SLA-11 gene, the SNP genotyping detection, and the genetic marker are obtained; the nucleotide sequence is shown in SEQ ID NO: 1-10; base mutations (C/T) exist at the position of 68 bp in the sequence table of SEQ ID NO: 1-10, and lead to the polymorphism of the MspI-RFLP. The invention further discloses a manufacturing method and application of the genetic marker.

The invention discloses a primer pair for screening mammalian experiment animal SNP (Single Nucleotide Polymorphism) markers and application thereof. 30 pairs of primer pairs are provided, and sequences are represented from SEQ ID NO:1 to SEQ ID NO:60; each primer pair can be used for amplifying genomic sequences of experiment animals including dogs, pigs, rats, mice, rhesus monkeys and rabbits under the same condition; and sequences are represented from SEQ ID NO:61 to SEQ ID NO:240. Sequence species amplified by the same pair of primers have difference. The application of the primer pair can greatly reduce the screening cost of the mammalian experiment animal genetic markers and simplify the operation, so that the standardization of experiment animal genetic quality monitoring is facilitated.

The invention belongs to the technical field of animal husbandry or animal genetic breeding and discloses identification and application methods for an MC1R gene haplotype. The identification method for the MC1R gene haplotype comprises the steps that an MC1R gene is subjected to PCR amplification, and single-ended Sanger sequencing is adopted for obtaining a variation sequence; based on the established haplotype, the genotype and phenotype of the hair color of a breeding group are subjected to association analysis, and the haplotype for controlling a black feather and the black feather homozygous genotype are identified. According to the identification method, the haplotype for controlling the black feather and the black feather homozygous genotype can be accurately identified. According to the identification method, key SNPs loci are simultaneously identified in one sequencing reaction; but in the prior art, two sequencing primers are used for simultaneously identifying the SNPs loci. Compared with a PCR-RFLP identification method in the prior art, through Sanger sequencing, the identification accuracy is further improved.

The invention belongs to the field of animal genetics and breeding, SNP (Single Nucleotide Polymorphism) markers and application and in particular relates to an SNP marker remarkably related to an Australian white sheep hoof color grade, a molecular marker and application. The marker is located on a 31603203rd nucleotide site (Chr19:31603203) on a No. 19 chromosome of an international sheep genome(Oar_v4.0) and the site has two alleles A and G and a 9th intron located on an MITF (Microphthalmia-Associated Transcription Factor) gene; a g.31603203A/G site has A/G polymorphism; an Australian white sheep hoof color of a GG gene type is black; the Australian white sheep hoof color of an AG gene type is grey; the Australian white sheep hoof color of an AA gene type is white. The SNP molecular marker provided by the invention can be used for assisting the breeding of an Australian white sheep new strain with uniform appearance and stable properties and cultivating a new variety by utilizingthe Australian white sheep.

The invention pertains to the technical field of animal genetic engineering, in particular to an application of the clone of a fat content gene Lpin1 in the muscle of the swine and an application of using the gene Lpin1 as a molecular mark. The invention is characterized in that: parts of a cDNA sequence and a DNA sequence of the fat content gene Lpin1 in the muscle of swine are cloned as listed in the sequence tables of SEQID NO:1 and SEQID NO:3. Through the application of management analysis, the C61-T61 in table SEQID NO:3 is found to have obvious relation with the fat content in the muscle. The invention discloses the Lpin1 gene sequence of the swine, the gene mutation loci influencing the fat content in the muscle and a preparation method to obtain the gene and an application of the gene. The invention provides a new molecular mark for the marker-assisted breeding of the swine.

The invention discloses a pig carcass deseription GFAT1 gene clone and polymorphism detection to mark auxiliary breeding in the animal genetic engineering technical domain, which is characterized by the following: the cDNA sequence of the pig carcass deseription GFAT1 gene is displayed as SEQ ID NO: 1 and the DNA sequence is depicted as SEQ ID NO: 2; the G101-A101 base at 101st position in the SEQ ID NO: 2 are mutated, which leads MvaI-RFLP polymorphism. The invention also provides a primer of 8th introne of the cloned GFAT1 gene and augumented GFAT1 to detect the polymorphism, which is a new genetic mark and method of polymorphism of the pig carcass deseription GFAT1 gene.

The invention relates to the animal genetics and the animal molecular cell biology, and provides an expression vector capable of efficiently expressing cow menin in eukaryotic cells. The expression vector capable of efficiently expressing cow menin in the eukaryotic cells provides necessary tools and means for the further research on the functions of the gene in the cells and the functional mechanism of the gene. The gene has expression in multiple organs in an organism and plays an important regulating role in regulating organism metabolism. According to the research on the functions and metabolic regulating and controlling pathways of the gene in a particular tissue or cell type, the fact that the gene is made to be excessively expressed under a specified condition by constructing the eukaryotic expression vector is an essential technological mean. The constructed efficient eukaryotic cell expression vector of the cow MEN1 gene (hereinafter referred to as bMEN1) can be used for providing necessary earlier research for the organism metabolic diseases of each tissue and organ of a cow, and even provides important tools and research means of a mode type research for the treatment of human diseases.

The invention belongs to the technical field of animal genetic engineering, and specifically relates to cloning, functional verification and application of two porcine adipose tissue-specific chimeric promoters. According to the invention, the activity of an adi-pro regulating element is verified, and different chimeric promoters containing a pCMVIE promoter and an SV40 enhancer are constructed through a chimeric method, so that the activity of the adi-pro regulating element is greatly improved and new specific expression promoter resources are provided for genetic engineering and molecular breeding. The nucleotide sequences of the promoters are respectively as shown in the sequence tables SEQ ID NO: 4 (sequence identity number: 4) and SEQ ID NO: 5.

The invention discloses a molecular marker related to chicken growth and development and application thereof, and belongs to the technical field of molecular marker-assisted selection and animal genetic breeding. The molecular marker is obtained by performing genome-wide association (GWAS) analysis on 741 test chickens of F2-generation resource groups established by Daweishan mini chickens and recessive Bailuoke broilers, a nucleotide single base mutation (named as Chr.3101578531A>G) of A >G exists at the 101578531bp position on the chromosome 3 of the version Gallus_gallus.GRCg6a of a chickenreference genome, a gene positioned in the nearby area is an RNA binding protein PUM2 (pumilio RNA binding family member 2) gene, and the mutation remarkably influences the weight of the chickens. The invention discloses obtaining and application of the molecular marker. The invention further provides a molecular marker genotyping detection method influencing chicken growth and development. By means of the method, an efficient and accurate molecular marker-assisted breeding technology can be established and applied to chicken growth and development genetic improvement, and therefore chicken growth and development are improved.

The invention belongs to the field of animal genetic engineering vaccines, and particularly relates to a fowl adenovirus serum type 4 (FAdV-4) recombinant virus for expressing fowl adenovirus serum type 8b (FAdV-8b) spike protein (Fiber) as well as a construction method and application of the fowl adenovirus serum type 4 (FAdV-4) recombinant virus. The recombinant virus is obtained by replacing a FAdV-4 Fiber1 gene with a Fiber gene of FAdV-8b, the recombinant virus can be used for preparing a bivalent vaccine for preventing and controlling chicken hepatitis-hydropericardium syndrome and/or chicken inclusion body hepatitis, and the bivalent vaccine prepared by utilizing the recombinant virus can achieve the effect of preventing two epidemic diseases at the same time by injecting a needle of vaccine. Therefore, the purpose of preventing and controlling two or more diseases by one needle can be achieved by inserting the FAdV-4 serving as a carrier into an exogenous gene.

The invention relates to a method for constructing a TAP gene-deleted pig T2 cell by using a CRISPR/Cas9 system, and belongs to the technical field of animal genetic engineering and genetic modification. The technical scheme mainly comprises the following steps: firstly, carrying out TAP2 gene knockout, connecting sgRNA for identifying TAP2 to a pUC57-sgRNA vector, transforming competent cells toobtain a pUC57-sgRNA plasmid containing a targeted recognition sequence, and screening positive clones through sequencing; co-transfecting a PK15 cell with a recombinant plasmid pUC57-sgRNA and a Cas9plasmid; and knocking out TAP1 on the TAP2 gene-knocked cell by using the same method. According to the method, a TAP gene-knocked PK15 cell line is constructed by using CRISPR/Cas9, a cell model isestablished for screening multiple pig-derived virus CTL epitopes, and meanwhile, a cell model is established for researching a TAP non-dependent presentation mechanism of exogenous antigens.

The invention belongs to the animal genetic engineering technical field, in particular relating to a construction for a framework plasmid of a recombinant baculovirus vector and application thereof. The invention acquires a framework plasmid pFB-G-SFV of a recombinant baculovirus transfer vector by cloning, and the plasmid is collected in China Center for Type Culture Collection (CCTCC) with the accession number of CCTCC NO: M207070. The invention also discloses the application of the plasmid pFB-G-SFV on the package of the recombinant baculovirus.

The invention relates to an economic crossbreeding method for increasing the benefit of a pure breeding pig farm, belonging to the field of animal genetic breeding. The economic crossbreeding method comprises the following steps of: 1) pure breeding: dividing a purely bred pig herd into two parts, i.e. a nucleus herd and a production herd, determining a scale of the nucleus herd according to a variety breeding target or a breed conservation plan, and taking pigs which do not function as the nucleus herd as the production herd; and 2) economic crossbreeding method: focusing on crossbreeding as for sows of the production herd, and alternately carrying out pure breeding and crossbreeding on sows of the nucleus herd. The crossbreeding advantages cannot be utilized in pure breeding of the pigs, and the economic benefit of the purely bred pigs is often lower, in particular to Chinese native pigs and pigs in bred variety. If economic crossbreeding is properly carried out in the pure breeding pig farm under the condition of ensuring to finish a pure breeding task, the economic benefit of the pig farm can be greatly increased.

The invention belongs to the technical field of animal genetic engineering, and particularly relates to separation identification and functional verification of promoter areas with different lengths of porcine skeletal muscle specific expression gene tnnc2. Five promoters with different lengths in the upstream of a porcine skeletal muscle specific expression gene tnnc2 are cloned from a porcine genome, and the nucleotide sequences are shown as SEQ ID NO: 1 to SEQ ID NO: 5; the result shows that tnnc2-pro fragment of 2313 bp and tnnc2-Q2 fragment of 1048bp have independent promoter activity and muscular tissue specificity, and the nucleotide sequences are shown as SEQ ID NO: 1 and SEQ ID NO: 3. The invention discloses a preparation method of five different deletion promoter fragments, a dual-luciferase activity detecting system and application to promoter activity analysis by a quantitative PCR method.