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Molecular marker related to chicken growth and development and application thereof

A growth and development, molecular marker technology, applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problems of low breeding efficiency, long breeding cycle, hybrid hybridization, etc. Efficiency, reduce breeding costs, reduce the effect of breeding cycle

Active Publication Date: 2020-11-10
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although high-quality chickens also adopt hybrid breeding technology, the hybrid matching lacks overall planning, and there are many hybrid hybrids.
This leads to low selection intensity, long breeding cycle, high breeding cost and low overall breeding efficiency in domestic chicken breeding
Moreover, the traditional breeding of chickens in China is based on the selection of quantitative traits such as growth rate and egg production. It is difficult to carry out precise directional selection, resulting in the inability to quickly carry out the breeding and promotion of fine varieties and effectively preserve and exchange chicken germplasm resources. Reduced efficiency of domestic chicken breeding

Method used

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  • Molecular marker related to chicken growth and development and application thereof
  • Molecular marker related to chicken growth and development and application thereof
  • Molecular marker related to chicken growth and development and application thereof

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0034] Experimental example 1 Experimental chicken flock feeding and body weight measurement

[0035] (1) Experimental animals

[0036] The test chicken group used in the present invention is the F2 generation resource group obtained by crossing Daweishan miniature chicken and recessive White Rock broiler chicken.

[0037] In the F0 generation, breeders were selected from Daweishan miniature chickens (from the practice chicken farm of Yunnan Agricultural University) and recessive white lock broilers (from Kunming Yunling Guangda Breeder Feed Co., Ltd.), and the selected male and female chickens had this breed Features, high egg production, medium weight, pure blood. Daweishan miniature chicken is a small chicken breed that has not been selected for breeding. It has the advantages of small size (average weight 0.7-1.2kg), low feed consumption, and low basal metabolism. It is a new breed of high-quality and grain-saving chickens (line ) of excellent material. Recessive white ...

experiment example 2

[0043] Experimental example 2 Acquisition of molecular markers related to chicken growth and development

[0044] (1) Blood sample collection

[0045]Vacuum blood collection tubes containing EDTA-dipotassium were used to collect blood from chicken wing veins.

[0046] (2) Extraction and identification of genomic DNA

[0047] Gentra Puregene Blood Kit (Qiagen) column centrifugation kit was used to extract blood genomic DNA. After the DNA was completely dissolved, its concentration was detected with a NannoDrop nucleic acid analyzer, and the integrity of the DNA band was detected under 1% agarose gel electrophoresis conditions. sex.

[0048] (3) DNA library construction and sequencing

[0049] The construction of the library was completed using the NEBNext DNA library kit (NewEngland Biolabs). The specific operation steps were as follows: firstly, the genomic DNA was randomly broken into small fragments, and then Illumina paired-end adapters were connected to the fragments wi...

experiment example 3

[0066] Experimental example 3 A kind of molecular marker genotype detection method that affects chicken growth and development

[0067] (1) Experimental animals

[0068] The experimental chicken group used in the present invention is 200 (100 male and female) of 150-day-old Daweishan miniature chicken breeders of Yunnan Agricultural University, which is the core group of breeder chickens. The chickens have free access to food and water, and the entire feeding method and feeding conditions are always consistent. Measure body weight.

[0069] (2) Target DNA sequence amplification and sequencing

[0070] 1) Primer design

[0071] The DNA sequence of chicken chromosome 3 was downloaded from the NCBI website, and primers were designed using the primer design software primer premier 6.0.

[0072] The DNA sequences of the designed primers are as follows:

[0073] Upstream primer primer-F: 5'-GCTTTGCCAGCCATCCAATC-3';

[0074] Downstream primer primer-R: 5'-AGCGTTGCCTGTTGTGTTTG-3...

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Abstract

The invention discloses a molecular marker related to chicken growth and development and application thereof, and belongs to the technical field of molecular marker-assisted selection and animal genetic breeding. The molecular marker is obtained by performing genome-wide association (GWAS) analysis on 741 test chickens of F2-generation resource groups established by Daweishan mini chickens and recessive Bailuoke broilers, a nucleotide single base mutation (named as Chr.3101578531A>G) of A >G exists at the 101578531bp position on the chromosome 3 of the version Gallus_gallus.GRCg6a of a chickenreference genome, a gene positioned in the nearby area is an RNA binding protein PUM2 (pumilio RNA binding family member 2) gene, and the mutation remarkably influences the weight of the chickens. The invention discloses obtaining and application of the molecular marker. The invention further provides a molecular marker genotyping detection method influencing chicken growth and development. By means of the method, an efficient and accurate molecular marker-assisted breeding technology can be established and applied to chicken growth and development genetic improvement, and therefore chicken growth and development are improved.

Description

technical field [0001] The invention relates to the field of molecular marker-assisted selection technology and animal genetic breeding technology, in particular to a molecular marker related to chicken growth and development and its application. Background technique [0002] Poultry meat has become one of the most important sources of meat in the world. As people's demand for high-quality broilers continues to increase, the breeding and multiplication of high-quality broilers is becoming more and more important to the development of the poultry industry. It is an important content of chicken breeding that humans select the phenotype of chickens to improve their production performance. At present, white-feathered broilers, which account for 50% of my country's broiler slaughter, are completely dependent on imports. Once the supply chain is broken, my country's broiler market will inevitably be severely hit. [0003] In recent years, developed countries have seized biologica...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6888C12Q1/6858C12Q2600/124C12Q2600/156C12Q2600/172C12Q2531/113C12Q2535/122Y02P60/87
Inventor 贾俊静豆腾飞闫世雄孙帅王坤黄英曹玮娜徐志强谷大海李琦华葛长荣荣华
Owner YUNNAN AGRICULTURAL UNIVERSITY
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