Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Molecular marker related to pH (Potential of Hydrogen) value character of pig muscle

A molecular marker and muscle technology, which can be used in the determination/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc. It can solve the problems of pH difference and affecting the speed of glycolysis.

Inactive Publication Date: 2015-04-01
HUAZHONG AGRI UNIV
View PDF0 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the activity of lactate dehydrogenase will directly affect the rate of glycolysis, resulting in the difference in pH

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Molecular marker related to pH (Potential of Hydrogen) value character of pig muscle
  • Molecular marker related to pH (Potential of Hydrogen) value character of pig muscle
  • Molecular marker related to pH (Potential of Hydrogen) value character of pig muscle

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Pig Genomic DNA Extraction

[0023] The pig breed for extracting the pig genome DNA in the present invention is Large White pig (Hubei Jinlin Original Breeding Animal Husbandry Co., Ltd.). Pig population DNA samples were all extracted using the Genomic DNA Kit (TIANamp Genomic DNA Kit) produced by Tiangen Biochemical (Beijing) Technology Co., Ltd. The specific operation steps are as follows:

[0024] 1. Put the ear sample of Large White pig into a 2mL EP tube, wipe clean ophthalmic surgical scissors with alcohol cotton and cut into a paste, then add 200 μL buffer GA (included with the kit).

[0025] 2. Add 20 μL proteinase K solution (included with the kit), mix well and place in a 56°C water bath for overnight digestion.

[0026] 3. Add 200 μL buffer GB, mix thoroughly by inversion, place at 70°C for 10 minutes, the solution should become clear, and centrifuge briefly to remove water droplets on the inner wall of the tube.

[0027] 4. Add 200 μL of absolute ethan...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of preparation of livestock molecular markers, and particularly relates to an SNP (Single Nucleotide Polymorphism) molecular marker related to a pH (Potential of Hydrogen) value character of pig muscle. The molecular marker is obtained by LDHA (Lactate DehydrogenaseHydroxyacyl) gene cloning and has a nucleotide sequence as shown in Figure 4.In the sequence as shown in Figure 4, the 495th position has allele G or A base substitution, and the 635th position of the sequence has allele C or T base substitution. The new molecular marker is provided for assistant selection of pig markers.

Description

technical field [0001] The invention belongs to the technical field of livestock molecular marker preparation, and in particular relates to the application of a molecular marker related to the pH value traits of pork. Background technique [0002] The main source of meat products in my country is pork. With the development of pig breeding industry, its output has been able to meet people's needs, so the improvement of pork quality has become a hot issue in the study of pig breeding. The improvement of meat quality can be considered from many aspects such as genetics, nutrition and disease prevention and treatment, among which genetic factors are the internal reasons. Breeding by means of molecular genetics can effectively improve the quality of pork from the source. [0003] Meat quality evaluation indicators include meat color, pH value, water resistance, drip loss, marbling, etc. , and the pH value is closely related to the meat color and water strength as well as the co...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/158
Inventor 赵书红黄济贺佳玮曹建华李世军李长春李新云
Owner HUAZHONG AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com