46 results about "MBHA resin" patented technology

The invention discloses a Telis-vasophysin preparing method of solid-phase polypeptide, which comprises the following steps: adopting Rink Amide resin (concluding Rink Amide MBHA resin, Rink AmideAM resin) as original material, Fmoc protective amino acid as monomer, TBTU or HBTU-to-HOBt as condensing agent to connect amino sequently; using Boc-Gly-OH for the last peptide chain; adding peptide cutting agent to cut peptide; adding ether to deposit to obtain crude product; adding alkali material to oxidate at 7.5-10.0 pH value to generate oxide crude product; proceeding separation and purifying through C18 (or C8) pillar to produce object product. The method possesses low manufacturing cost, simple technology, high obtaining rate and low environmental pollution, which is convenient to do industrial construction.

A method for realizing the solid phase synthesis of cetrorelix comprises the following steps that: Fmoc-Linker-MBHA-Resin is used as a starting raw material and is connected in turn with amino acid with Fmoc-protecting groups according to a solid phase synthesis method, so as to obtain protective decapeptide resin; meanwhile, the Fmoc-protecting groups are divested in turn; one sort of HBTU / HOBT or DIC / HOBT is used as condensing agent to carry out synthesized peptide, thereby obtaining protective decapeptide resin; acetylation reaction is carried out, and then protecting group side chain divesting and peptide cutting are carried out synchronously to obtain cetrorelix acetate which is separated and purified through C18 or C8 chromatographic column; finally, cetrorelix acetate acetate or trifluoroacetate is obtained after freeze drying.

The invention discloses a preparation method of acetic acid redfish calcitonin. The preparation method comprises the following steps of: deprotecting Rink Amide MBHA resin by using a deprotection reagent and removing a Fmoc protecting group; sequentially coupling the deprotected Rink Amide MBHA resin serving as a starting material with Fmoc-protected amino acids serving as monomers to obtain acetic acid redfish calcitonin peptide resin, wherein a condensing agent is N,N-diisopropyl carbodiimide (DIC) / 1-hydroxybenzotriazole (HOBt) or benzotriazol-1-yl-oxytripyrrolidinophosphonium hexafluorophosphate (PyBOP) / HOBt; splitting the acetic acid redfish calcitonin peptide resin, adding diethyl ether and precipitating the acetic acid redfish calcitonin peptide resin to obtain reduction type acetic acid redfish calcitonin crude peptide; cyclizing the reduction type acetic acid redfish calcitonin crude peptide to obtain oxidation type acetic acid redfish calcitonin crude peptide; and performing purification, salt conversion, concentration and freeze drying on the oxidation type acetic acid redfish calcitonin crude peptide to obtain the acetic acid redfish calcitonin. According to the preparation method, the yield of the acetic acid redfish calcitonin reaches over 20 percent.

The invention relates to a solid-phase synthesis process of a thymosin alpha 1, belonging to the polypeptide solid-phase synthesis technical field. The invention comprises the following steps: a. a Fmoc-Rink Amide AM resin or a Fmoc-Rink Amide MBHA resin is used as carrier, an H2N-Rink Amide AM resin or an H2N-Rink Amide MBHA resin is obtained after deprotection of the Fmoc; b. side chain carboxyl group of Fmoc-Asp-X is connected with resin amino by the method of solid-phase synthesis to obtain the Fmoc-Asp (resin)-X; c. the left amino acid in the sequence is synthesized in solid-phase with the Fmoc strategy; d. after the amino protection group Fmoc of N terminal amino acid is removed, the N terminal amino acid is acetylated by acetic anhydride and pyridine; e. then the acetylated N terminal amino acid is cut by a cracking agent (tri fluoroacetic acid / benzoylate sulfide / 1, 2- dithioglycol / Anisole) to obtain the thymosin alpha 1; f. crude product of the thymosin alpha 1 is prepared and separated by HPLC to obtain the pure thymosin alpha 1. The invention can significantly increase the yield of the thymosin alpha 1 and decrease the production cost, which is helpful for scale production and has better industrialization prospect.

The invention relates to a solid phase synthesis method for ziconotide, which comprises the following steps: an Fmoc-Rink Amide-MBHA resin is taken as a solid phase carrier for program reaction; condensation reactions are orderly performed to link 25 amino acids with protecting groups to obtain a linear peptide complete-protection resin of the 25 amino acids,wherein in three groups of Cyses which form a disulfide bond, the Cyses in the same group are simultaneously linked with one of a Trt, Acm or Mob protecting group, and different groups of the Cyses are linked with different protecting groups; the protecting groups of the three groups of the Cyses are orderly removed; a cyclization reaction is performed to form a disulfide ring bond; a side chain protecting group is removed; and the resin is cut to obtain the ziconotide containing three disulfide rings. The method has the main advantages of few side products, high directive efficiency, simplicity, convenience, high yield of products, and favorability for the purification of the products.

The invention discloses a solid phase synthesis process for a ATL peptide. The process comprises using MBHA resin as a solid phase carrier, combines protective lysyl Boc-Lys(2-Cl-Z) on the resin, completing the synthesis of ATL by stepwise peptide combining method for protecting aminophenol with Boc, modifying N end with acetic anhydride by acetylizing to obtain all-protected peptide resin; depriving the side chain protecting group of the formacyl of Trp with piperidine, and at last, depriving side chain protection group with fluoroform sulfonic acids and incising the resin to obtain ATL coarse peptide; and then purifying the crude product with the highly effective liquid phase of reversed phase C18 filler, separating and purifying with methanol-water gradient eluting process to obtain ATLpeptide refining product. The process of the invention with single-step condensation reaction has high yield and low by-product, and the final crude product has high purity and small impurity content, the operation is simple, and the total yield is high.